A large-visual-field-angle multilayer conjugate self-adaptive optical focusing and microscopic system and method

A technology of adaptive optics and microscopic systems, applied in optics, microscopes, optical components, etc., can solve the problem of effectively correcting the small field of view, achieve an excellent experimental basis, overcome the small field of view, and improve focusing and imaging quality Effect

Active Publication Date: 2017-09-01
ZHEJIANG UNIV
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Problems solved by technology

This kind of adaptive optics system can only correct the relatively constant aberration introduced by the optical system, and both biological tissue and atmospheric turbulence are generally three-dimensional objects, and the aberrations generated at different positions also vary in space, so it is very difficult to effectively correct the field of view angle. Small

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  • A large-visual-field-angle multilayer conjugate self-adaptive optical focusing and microscopic system and method
  • A large-visual-field-angle multilayer conjugate self-adaptive optical focusing and microscopic system and method
  • A large-visual-field-angle multilayer conjugate self-adaptive optical focusing and microscopic system and method

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[0042] The present invention will be described in detail below in conjunction with the embodiments and accompanying drawings, but the present invention is not limited thereto.

[0043] like figure 2 As shown, the present invention specifically includes a laser 1, a first collimating beam expander lens 2, a second collimating beam expander lens 3, a first converging lens 4, and a first conjugate spatial light modulator arranged in sequence along the same optical axis. 5. First collimating lens 6, second converging lens 7, second conjugate spatial light modulator 8, second collimating lens 9, pupil type spatial light modulator 10, focusing lens 11, first scattering medium 12. The second scattering medium 13 and the focusing imaging system. The light emitted from the laser 1 passes through the first collimating beam expander lens 2 and the second collimating beam expander lens 3 and then enters the first converging lens 4. The outgoing light of the first converging lens 4 passe...

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Abstract

The invention discloses a large-visual-field-angle multilayer conjugate self-adaptive optical focusing and microscopic system and method. The light emitted from a laser is incident on three spatial light modulators; the light beam modulated by the spatial light modulators is focused on a scattering medium and then imaged by an imaging lens; the scattering medium is divided into two layers including two conjugate spatial light modulators and a pupil-type spatial light modulator; the two conjugate spatial light modulators are respectively conjugated to the two layers of the scattering medium; and the pupil-type spatial light modulator is arranged among the two conjugate spatial light modulators and the scattering medium. The system and method of the invention use a plurality of guide stars and a plurality of wave-front correctors to correct the aberrations generated at different thicknesses of biological samples at the same time, overcome the disadvantages that the traditional adaptive optical imaging technology has a small viewing angle, a poor non-target point correction effect and a low correction speed, and can realize large-view-field, multi-zone, high-quality focus and high-resolution microscopic imaging.

Description

technical field [0001] The invention relates to the field of optical microscopic imaging based on an adaptive algorithm, in particular to a large field angle multilayer conjugate adaptive optical focusing and microscopic system and method. Background technique [0002] In biomedical research, aberrations caused by the inhomogeneity of the refractive index of biological tissue samples seriously affect the imaging quality of biological samples, and as the thickness of the observable biological sample increases, ideal focusing becomes more difficult. Applying adaptive optics technology to the field of biomedical imaging, deformable mirrors or spatial light modulators can be used to compensate and correct the distorted wavefront, so as to achieve focusing inside or penetrating biological tissues, and obtain diffraction Images with extreme resolution. [0003] In the traditional adaptive optics technology, a single wavefront corrector is used to correct the distortion generated ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B27/00G02B21/00G02B27/46
CPCG02B21/0072G02B27/0025G02B27/46
Inventor 斯科龚薇赵琪
Owner ZHEJIANG UNIV
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