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A screening method for essential oils with refreshing effect or products thereof

A screening method and technology for essential oils, applied in the field of daily chemicals, can solve problems such as inability to cure, quantify biological efficacy, and lack thereof, and achieve the effects of easy operation, repeatable data, and safe application.

Active Publication Date: 2018-11-09
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technical means for evaluating the biological efficacy of aromatic essential oils lack the support of objective standards at the biological level, and cannot solidify and quantify the relevant biological efficacy of the refreshing effect of aromatic essential oils, resulting in the selection of aromatic essential oil raw materials with refreshing effects in industrial applications. , formula application and efficacy publicity, etc., are subject to great limitations and uncertainties

Method used

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  • A screening method for essential oils with refreshing effect or products thereof
  • A screening method for essential oils with refreshing effect or products thereof
  • A screening method for essential oils with refreshing effect or products thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 The cultivation of brain neuron cells

[0049] 1. Culture primary mouse brain neurons

[0050] Select newborn 1-day-old mice, separate and extract neuronal cells in the mouse brain, disperse them in Neurobasal Medium (Thermo Fisher, Cat.#21103-049) culture medium containing horse serum, and then place them in a 37°C cell culture incubator Culture, the culture condition is air: carbon dioxide is 95:5. Change the brain neuron cell culture medium every other day. The growth status of brain neuron cells on the 10th day of culture is as follows: figure 1 shown.

[0051] 2. Culture primary human brain neurons

[0052] Human brain neuron cells were purchased through commercial channels, and the cells were placed in Neurobasal Medium (Thermo Fisher, Cat. #21103-049) culture medium containing horse serum, and then placed in a 37°C cell incubator for culture. The culture conditions were as follows: Air: carbon dioxide is 95:5. Change the brain neuron cell culture...

Embodiment 2

[0053] Example 2 Effects of Peppermint Essential Oil on the Release Levels of 3 Neurotransmitters in Brain Neuron Cells

[0054] Mint Peppermint Yakima Oil affects time on neurotransmitter release levels in brain neurons. Add peppermint essential oil to the brain neuron cell culture system on the 10th day of cultivation, and make the final concentration of peppermint essential oil in the brain neuron cell culture system be 0.0005%, 0.0050% and 0.0500%, measure the peppermint essential oil placed in the brain neuron cell The levels of dopamine (DOPAMINE), γ-aminobutyric acid (GABA) and melatonin (MELATONIN) released by neuronal cells in the culture medium after 0-60 minutes. The neurotransmitter sample collection time in this embodiment is the 5th minute, the 10th minute, the 15th minute, the 20th minute, the 30th minute, the 40th minute, the 50th minute and the 60th minute. The result is as Figure 2-4 shown.

[0055] The test results show that the release time of peppermin...

Embodiment 3

[0056] Example 3 Effects of Peppermint Essential Oil on the Release Levels of Various Neurotransmitters in Brain Neuron Cells

[0057] At different concentrations, the effect of peppermint essential oil (Mint Peppermint Yakima Oil) on the release levels of different neurotransmitters in the neuron cell culture system at the 30th minute of the brain neuron cells is shown in Table 1 below. The neurotransmitters involved in this embodiment include dopamine (DOPAMINE), serotonin (5HYDROXY-TRYPTAMINE), γ-aminobutyric acid (GAMMA AMINOBUTYRIC ACID), acetylcholine (ACETYLCHOLINE), norepinephrine (NORADRENALINE), glycine (GLYCINE) , glutamate (GLUTAMINE) and melatonin (MELATONIN).

[0058] Table 1 Effects of peppermint essential oil on the release levels of different neurotransmitters in brain neurons

[0059]

[0060] The above test results show that at a certain concentration, peppermint essential oil can promote the release of melanin, dopamine, and norepinephrine, while inhibi...

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Abstract

The invention relates to the technical field of daily chemicals, in particular to a screening method for essential oils with refreshing effect or products thereof. The screening method includes: using essential oils or their products to treat brain neuron cells, detecting the concentration of serotonin, etc., to obtain the concentration of neurotransmitters in the treatment group; detecting the changes in the concentration of neurotransmitters in the treatment group compared with the concentration of neurotransmitters in the blank control group rate Vc; get the refreshment score Xi of each neurotransmitter according to Vc; detect the total refreshment score X of the essential oil or its product according to the summation formula; when X≥40, the essential oil or its product is an essential oil with refreshing effect or its products. The screening method of the present invention is aimed at the biological in vitro evaluation of the refreshing effect of the aromatic essential oil and its products, and has the characteristics of fast and accurate, repeatable data, curable method, objective standard and safe application.

Description

technical field [0001] The invention relates to the technical field of daily chemicals, in particular to a screening method for essential oils with refreshing effect or products thereof. Background technique [0002] With the development of society, the pace of modern life is accelerating, the competition for survival is becoming increasingly fierce, and the pressure of work and study is increasing. Especially business people, white-collar workers and students often feel tired and dizzy. Some people are prone to sleepiness in spring, hot summer, and autumn, especially drivers, who need to refresh their minds when they are tired and sleepy to avoid danger. As a result, consumers have increased demand for products with refreshing effects. [0003] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots or fruits of plants through steam distillation, extrusion, cold soaking or solvent extraction. Aroma essential oils can have an effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50
CPCG01N33/5058A61P25/00
Inventor 肖蕾黄金莲陈宇
Owner INFINITUS (CHINA) CO LTD
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