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Refreshing essential oil or product screening method

A screening method and essential oil technology, applied in the field of daily chemicals, can solve problems such as uncurable, lack, and quantitative biological efficacy, and achieve the effects of easy operation, repeatable data, and accelerated screening and evaluation progress

Active Publication Date: 2017-09-08
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technical means for evaluating the biological efficacy of aromatic essential oils lack the support of objective standards at the biological level, and cannot solidify and quantify the relevant biological efficacy of the refreshing effect of aromatic essential oils, resulting in the selection of aromatic essential oil raw materials with refreshing effects in industrial applications. , formula application and efficacy publicity, etc., are subject to great limitations and uncertainties

Method used

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  • Refreshing essential oil or product screening method
  • Refreshing essential oil or product screening method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Cultivation of brain neuron cells

[0049] 1. Culturing primary mouse brain neuron cells

[0050] Select 1 day newborn mice, isolate and extract neuron cells from mouse brains, disperse them in Neurobasal Medium (Thermo Fisher, Cat.#21103-049) culture medium containing horse serum, and then place them in a 37℃ cell incubator. Cultivation, the culture condition is air: carbon dioxide 95:5. Change the brain neuron cell culture medium every other day. The growth status of brain neuron cells on the 10th day of culture is as figure 1 Shown.

[0051] 2. Culturing primary human brain neuron cells

[0052] Purchase human brain neuron cells through commercial channels, and place the cells in NeurobasalMedium (Thermo Fisher, Cat.#21103-049) culture medium containing horse serum, and then place them in a 37°C cell incubator for culture under the following conditions: Air: carbon dioxide is 95:5. Change the brain neuron cell culture medium every other day. Growth status of b...

Embodiment 2

[0053] Example 2 Effect of peppermint essential oil on the release level of three neurotransmitters in brain neurons

[0054] Mint Peppermint Yakima Oil affects the time of neurotransmitter release from brain neurons. Add peppermint essential oil to the brain neuron cell culture system on the 10th day of culture, and make the final concentration of peppermint essential oil in the brain neuron cell culture system 0.0005%, 0.0050% and 0.0500%, and measure the peppermint essential oil to place the brain neuron cells The levels of dopamine (DOPAMINE), γ-aminobutyric acid (GABA) and melatonin (MELATONIN) released by neuronal cells in the culture medium after 0-60 minutes. The neurotransmitter sample collection time in this embodiment is the 5th minute, the 10th minute, the 15th minute, the 20th minute, the 30th minute, the 40th minute, the 50th minute, and the 60th minute. The result is Figure 2-4 Shown.

[0055] The test results show that the time for peppermint essential oil to rel...

Embodiment 3

[0056] Example 3 Effect of peppermint essential oil on the release level of various neurotransmitters in brain neurons

[0057] At different concentrations, the effects of Mint Peppermint Yakima Oil on the release levels of different neurotransmitters in the neuronal cell culture system at the 30th minute of brain neuron cells are shown in Table 1 below. The neurotransmitters involved in this embodiment include dopamine (DOPAMINE), serotonin (5HYDROXY-TRYPTAMINE), gamma-aminobutyric acid (GAMMA AMINOBUTYRIC ACID), acetylcholine (ACETYLCHOLINE), norepinephrine (NORADRENALINE), glycine (GLYCINE) , Glutamate (GLUTAMINE) and Melatonin (MELATONIN).

[0058] Table 1 Effects of peppermint essential oil on the release levels of different neurotransmitters in brain neurons

[0059]

[0060] The above test results show that at a certain concentration, peppermint essential oil can promote the release of melanin, dopamine, and norepinephrine, while inhibiting the release of serotonin, γ-aminobu...

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Abstract

The invention relates to the technical field of household chemicals, particularly to a refreshing essential oil or product screening method. The refreshing essential oil or product screening method comprises processing cerebral nerve cells through essential oil or products, detecting concentration of materials such as 5-hydroxytryptamine to obtain the neurotransmitter concentration of a treated group; detecting the change rate Vc by comparing the neurotransmitter concentration of the treated group with that of a blank control group; according to Vc, separately acquiring the refreshing score Xi of every neurotransmitter; according to a summation formula, detecting the total refreshing score X of the essential oil or product; and when X is greater than or equal to 40, determining that the essential oil or product is an essential oil or product with refreshing effects. The refreshing essential oil or product screening method is applied to performing refreshing effect in-vitro biological evaluation on aroma essential oils or products and has the advantages of being rapid, accurate, repeatable in data, solidifiable in method, objective in standard and safe in application.

Description

Technical field [0001] The invention relates to the technical field of daily chemicals, in particular to a method for screening essential oils or their products with refreshing effects. Background technique [0002] With the development of society, the pace of modern life is accelerating, the competition for survival is becoming increasingly fierce, and the pressure of work and study is increasing, especially for business people, white-collar workers and students, who often feel tired and dizzy. Some people are prone to get sleepy in spring, hot summer, and autumn. Drivers in particular need to be refreshed when tired and sleepy to avoid danger. Therefore, consumer demand for products with refreshing effects has increased. [0003] Essential oils are volatile aromatic substances extracted from plant flowers, leaves, stems, roots or fruits through steam distillation, extrusion, cold soaking or solvent extraction. Aromatic essential oils can have an effect on the human central nerv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5058A61P25/00
Inventor 肖蕾黄金莲陈宇
Owner INFINITUS (CHINA) CO LTD
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