Method for cryopreservation of donkey semen

A technology for cryopreservation and semen, applied in the biological field, can solve problems such as inconvenience in production and tight operation time, and achieve the effects of improving utilization efficiency, prolonging storage time, and prolonging storage time.

Inactive Publication Date: 2017-09-22
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
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Problems solved by technology

[0003] Donkeys belong to the genus Equus, and they are long-day seasonal estrus animals. For large-scale donkey farms, the estrus of female donkeys usually occurs in a concentrated manner. Artificial insemination after dilution, the operation time is relatively tight, which brings great inconvenience to production

Method used

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Embodiment Construction

[0024] The implementation of the present invention is described in further detail below in conjunction with examples, and the following examples are only descriptive, not limiting, and cannot limit the protection scope of the present invention with this.

[0025] A method for cryopreservation of donkey semen, the steps of the method are as follows:

[0026] (1) Configuration and processing of semen diluent

[0027] ① Configuration of the base solution: Accurately weigh 3.04g of Tris, 1.70g of citric acid, 1.50g of glucose, 1.50g of fructose, 0.06g of penicillin, and 0.10g of streptomycin with an electronic balance, and add 100mL of double distilled water to constant volume to prepare the base solution .

[0028] ②Preparation of egg yolk liquid: Eggs come from chicken farms free of disease. Make sure that the eggs are fresh (1-day-old eggs) and the eggshells are clean and pollution-free. Before taking the yolk, carefully disinfect the surface of the eggshells with 75% alcohol,...

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Abstract

The invention relates to a method for cryopreservation of donkey semen. The method comprises (1) preparing a base solution from 3.04 parts by mass of Tris, 1.70 parts by mass of citric acid, 1.50 parts by mass of glucose, 1.50 parts by mass of fructose, 0.06 parts by mass of penicillin, 0.10 parts by mass of streptomycin and 100 parts by mass of double distilled water and preparing a semen diluent from 85 parts by mass of the base solution and 15 parts by mass of an egg yolk solution, (2) using a rutting mare as a platform ass and collecting semen of a stud ass through a false vaginal method, wherein a motility rate is greater than 0.70, (3) taking the semen diluent and semen at 28 DEG C and carrying out dilution according to a ratio of 2: 1, (4) putting the semen diluent into a centrifuge tube, putting the centrifuge tube in a water bath at 28 DEG C and transferring the centrifuge tube into a refrigerator at 4 DEG C, and (5) in cryopreservation, carrying out uniform mixing every 24 hours and simultaneously detecting sperm viability through a microscope. The method obviously prolongs semen storage time, solves the problem of a large amount of artificial insemination in mare concentrated rutting in a large-scale donkey field, and effectively improves the utilization efficiency of the stud ass.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for low-temperature preservation of donkey semen. Background technique [0002] Livestock semen preservation combined with artificial insemination technology can greatly improve the reproductive utilization efficiency of breeding males. Generally, the preservation of mammalian semen can be divided into normal temperature preservation (15-25°C), low temperature preservation (4°C) and cryopreservation (-196°C or -79°C). Room temperature storage was the first to develop, and the method is relatively simple, but the room temperature storage time is short, and it is easily contaminated by microorganisms, which significantly reduces the sperm viability and affects the conception rate. Cryopreservation can achieve the purpose of long-term preservation of sperm, but compared with room temperature and low temperature storage, the method of cryopreservation of semen is more complicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0231A01N1/0284
Inventor 刘新峰郭宏丁向彬
Owner TIANJIN AGRICULTURE COLLEGE
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