Culture medium apparatus provided with anaerobic device, and culturing method

Abstract

The invention relates to the technical field of microbial culture, and concretely relates to a culture medium apparatus provided with an anaerobic device, and a culturing method. The problems of provision of an effective anaerobic environment in the anaerobic culture process, and complicated sterilization and subpackaging extraction during culture experiments of a large amount of microbes are solved in the invention, and the problems of cross pollution between / among different culture media and the bacterial pollution risk brought in the culture medium packaging process are also prevented. The apparatus is disclosed against the problems during the storage of a liquid culture medium and can conveniently provide the anaerobic environment. The culture medium apparatus provided with the anaerobic device is a closed container, the closed container comprises a culture medium storage space and an anaerobic bag storage space; the culture medium storage space mutually communicates with the anaerobic bag storage space; and the closed container is provided with a seal membrane structure.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a culture medium device with an anaerobic device and a cultivation method. Background technique [0002] At present, there are many types of culture media used for microbial culture. For the cultivation of some anaerobic samples, the existing methods have their defects. The existing methods are mainly mechanical ventilation methods, including the following steps 1. Vacuum calibration 2. Replace nitrogen (99.99%) in the operating room for the first time, 3. Replace the operating room for the second time, and repeat the nitrogen filling process. Catalyze deoxygenation, and observe its discoloration after one hour with a melanin indicator. If the discoloration does not change, the anaerobic environment is reached in the operation room; 5. The third replacement of the mixed gas in the operation room (hydrogen 10%, nitrogen 80-85%, CO2 5 -10%); 6. Turn on the ultrav...

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Problems solved by technology

[0009] In order to overcome the problem of providing an effective anaerobic environment during the anaerobic culture process, as well as the sterilization of the medium and the troublesome problems of subpackaging and extraction during the large-scale microbial culture experiment, as well as the prevention of cross-contamination between different culture media, and due to the culture The risk of bacterial contamination brought during the base packing process is aimed at these problems in the storage of liquid medium, and the provision of anaerobic environment is easy to operate; the invention provides a liquid medium storage device, including the following content: a A culture medium device with an anaerobic device, the culture medium device is a closed container, and the closed space includes a culture medium storage space and an anaerobic bag storage space;

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