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Establishment method and application of eukaryotic avian leukosis virus capsid protein expression vector with immunosuppressive effect

A technology of eukaryotic expression vector and avian leukosis virus, applied in the biological field of virus-encoded protein expression and functional research

Pending Publication Date: 2017-11-21
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In addition, there are few reports on the biological function of ALV p27
[0006] Generally speaking, there are few reports on the biological function of p27 protein of avian leukosis virus at home and abroad, and there is no report on the immunosuppressive function of p27 protein

Method used

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  • Establishment method and application of eukaryotic avian leukosis virus capsid protein expression vector with immunosuppressive effect
  • Establishment method and application of eukaryotic avian leukosis virus capsid protein expression vector with immunosuppressive effect
  • Establishment method and application of eukaryotic avian leukosis virus capsid protein expression vector with immunosuppressive effect

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with specific embodiments, and the advantages and characteristics of the present invention will become clearer along with the description. However, these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0022] 1. Construction and identification of eukaryotic expression vectors:

[0023] The pGEX-6p-1-p27 / BL21 plasmid was digested with BamH I and Xho I, and connected to the pcDNA3.1(+) empty vector after gel recovery. The positive cloning plasmids identified by enzyme digestion were sent to Shanghai Yingjun Biotechnology Co.,...

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Abstract

The invention discloses an establishment method and application of an eukaryotic avian leukosis virus capsid protein expression vector with an immunosuppressive effect, and relates to the biotechnical field of expression and function research of virus encoding protein. The establishment method comprises the following steps: taking a pGEX-6p-1-p27 / BL21 plasmid as a template, performing PCR amplification by using specific primers, recycling PCR fragments, and linking with a pcDNA3.1 vector to obtain a positive recombinant plasmid, namely the eukaryotic expression vector with the function of suppressing immune response of cells to LPS and Poly (I: C) stimulation. The establishment method is simple and reasonable; after transfected cell expression, the eukaryotic expression vector can be applied to suppression of cellular immune response to the LPS or Poly (I: C) stimulation and provides a target for research and development of an anti-avian leukemia immunosuppressing medicine.

Description

technical field [0001] The invention relates to the field of biological technology of virus coded protein expression and function research. Background technique [0002] Avian leukosis (AL) is one of the important tumor diseases of poultry caused by avian leukosis virus (ALV). In addition to causing direct damage to chickens, virus infection also causes severe immunosuppression, decreased productivity, mixed infection, secondary infection, etc., resulting in huge economic losses and seriously endangering the development of the poultry industry. [0003] The sequence of the structural gene of avian leukosis virus (ALV), that is, gag / pro-pol-env from the 5th end to the 3rd end of the RNA molecule, these genes encode virus group-specific antigenic protein, protease, and RNA-dependent DNA polymerase respectively (reverse transcriptase or RT) and envelope glycoproteins. The terminal gene sequences on both sides of the structural gene have promoter and enhancer activities, formi...

Claims

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Application Information

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IPC IPC(8): C12N15/66C12N15/79
CPCC12N15/66C12N15/79
Inventor 钱琨孙舒秦爱建程晓薇孔正茹顾晨曦武宗仪
Owner YANGZHOU UNIV
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