Pentacyclic triterpene saponin compounds with anti-breast cancer activity in spina gleditsiae and extraction method thereof

A pentacyclic triterpenoid saponin, extraction method technology, applied in the directions of steroids, sexual diseases, antitumor drugs, etc., can solve the problem of inability to know the cytotoxic activity of cancer cells, and achieve the effect of significant anti-breast cancer activity

Active Publication Date: 2017-11-24
SHANDONG ANALYSIS & TEST CENT
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  • Abstract
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Problems solved by technology

However, since there is no research on the ingredients in the saponin thorn, it is im...

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  • Pentacyclic triterpene saponin compounds with anti-breast cancer activity in spina gleditsiae and extraction method thereof
  • Pentacyclic triterpene saponin compounds with anti-breast cancer activity in spina gleditsiae and extraction method thereof
  • Pentacyclic triterpene saponin compounds with anti-breast cancer activity in spina gleditsiae and extraction method thereof

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[0045] It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0046] It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinations thereof.

[0047] The preparation method of described new compound-saponin A, the steps are as follows:

[0048] (1) G...

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Abstract

The invention discloses pentacyclic triterpene saponin compounds with anti-breast cancer activity in spina gleditsiae and an extraction method thereof. The extraction method comprises the following steps: (1) grinding spina gleditsiae, and using an organic extracting agent to carry out heating reflux extraction and concentration to obtain a crude extract of spina gleditsiae; (2) dispersing the crude extract obtained in the step (1) into water, extracting the crude extract by petroleum ether and ethyl acetate in sequence to obtain a water phase, and concentrating the water phase to obtain a water phase part; (3) diluting obtained water phase part by water, making diluted water phase part go through an adsorbent resin chromatographic column to carry out adsorption, then eluting the adsorbent resin chromatographic column by an ethanol-water system in a gradient elution mode, and concentrating each elution part until no water is left; (4) making the 60% ethanol-water elution part go through a silica gel chromatographic column to carry out adsorption, and eluting the silica gel chromatographic column by a CH2Cl2-MeOH system in a gradient elution mode; and (5) making the elution part with a volume ratio of CH2Cl2 to MeOH of 1:1 go through a RP-C18 medium pressure chromatographic column, eluting the RP-C18 medium pressure chromatographic column by a methanol-water system in a gradient elution mode, and saving the 80% methanol-water elution part to prepare spina gleditsiae saponin A by using CH3CN/H2O (26:74 v/v).

Description

technical field [0001] The invention belongs to the technical field of saponins and preparation methods thereof, in particular to a pentacyclic triterpene saponins with anti-breast cancer activity in saponins and an extraction method thereof. Background technique [0002] Saponins are widely distributed in plants such as ginseng, Ophiopogon japonicus, Tianqi, Bupleurum, licorice and other marine organisms such as sea cucumbers and soft corals. According to the structure of aglycones, they can be divided into triterpenoid saponins and steroidal saponins. Among them, triterpene saponins are the most widely distributed, and can be divided into tetracyclic triterpene saponins and pentacyclic triterpene saponins, the latter being the most common. Triterpene saponins have good anti-tumor effects in vivo and in vitro, and their anti-tumor mechanisms are diverse. They can not only directly inhibit the growth of tumor cells, but also enhance the body's immunity, inhibit tumor invasio...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61P35/00A61P15/14
CPCC07J63/008
Inventor 于金倩王晓段文娟王岱杰耿岩玲
Owner SHANDONG ANALYSIS & TEST CENT
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