A kind of preparation method and application of diphenylmethane compound
A technology of diphenylmethane and compounds, which is applied in the field of screening out active ingredients of anti-breast cancer cells, can solve problems such as undiscovered pharmacological activity research reports, and achieve enhanced anti-breast cancer cell activity, simple operation, and high yield Effect
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Embodiment 1
[0032] Embodiment 1: the method for preparing monomer compound from Euphorbia chamaejasma
[0033] 30kg of dried Euphorbia chamaejasma, crushed, soaked in 80% (ethanol: water 80:20) ethanol reflux extraction twice, each time for 2 hours, the amount of solvent was 180L and 150L respectively. Combine the extracts, filter while hot, combine and concentrate until no ethanol remains, and concentrate the volume to 8L. Take 2L of the concentrated solution and add 1L of water to dilute it, then extract 3 times with petroleum ether, ethyl acetate and n-butanol in sequence, each time the extraction solvent is 3L, and recover the solvent under reduced pressure to obtain 60g of petroleum ether and 160g of ethyl acetate , 20g of n-butanol.
[0034] The ethyl acetate part was passed through a normal phase silica gel column and eluted with petroleum ether: ethyl acetate 100:1, 50:1, 20:1, 15:1, 10:1, 5:1, 1:1 to obtain Fr. 1-Fr. 7.
[0035] Take 12g of Fr.2 to pass through the normal phas...
Embodiment 2
[0057] Embodiment 2: Anti-breast cancer cell experiment in vitro
[0058]MTT method was used to evaluate cytotoxicity, and 6 types of breast cancer cells were selected for research, namely triple negative breast cancer MDA-MB-231, Sum149, LuminalA type MCF-7, LuminalB type ZR-75-1, Her 2 positive type SKBr3, BT474. Paclitaxel was used as the positive control drug. Adjust the above six kinds of breast cancer cell density to 5 × 10 4 cells / ml, inoculate into 96-well culture plate, inoculate 100 μL cell suspension in each well, place at 37°C, 5% CO 2 Cultivate overnight in an incubator, observe that the cells adhere to the wall and grow well, and then set up a positive control group, a tumor cell control group and an experimental group with different concentrations of drugs. The experimental group added 8 sample solutions with gradient concentrations. The concentration of compound 1 and 2 mother solutions was 5mM, and the concentration of paclitaxel mother solution was 3.5mM. ...
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