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Denitrification bacterium biological sphere for hairy crab breeding water purification system and preparation method of denitrification bacterium biological sphere

A technology of denitrifying bacteria and water purification system, which is applied in the field of nitrifying bacteria biospheres and its preparation, and can solve problems such as water purification

Inactive Publication Date: 2017-11-24
苏州市阳澄湖蟹王水产有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Technical problem: Provide denitrifying bacteria biospheres used in hairy crab breeding water purification system to solve the water purification problem of artificial hairy crab breeding in the prior art

Method used

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  • Denitrification bacterium biological sphere for hairy crab breeding water purification system and preparation method of denitrification bacterium biological sphere

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the cultivation method of denitrifying bacteria.

[0019] (1) Denitrifying bacteria ATCC 14581 is activated on the slant medium; the formula of the slant medium is as follows: beef extract 5.0g / L, peptone 10.0g / L, NaCl 5.0g / L, adjust pH 7.2~ 7.4.

[0020] (2) The activated denitrifying bacteria ATCC 14581 was inoculated into the organic carbon source denitrification liquid medium for culture, and the culture conditions were: 30-37° C., 170-200 rpm shaking culture for 24-36 hours. The formula of described organic carbon source denitrification liquid medium is as follows: KH 2 PO 4 0.7g / L, MgSO 4 ·7H 2 O 0.5g / L, CaCl 2 2H 2 O 0.5g / L, (NH 4 ) 2 SO 4 0.5g / L, glucose 2.0g / L, NaOH to adjust the pH to 7.2-7.4, and the solvent is water.

Embodiment 2

[0021] Embodiment 2: TiO2 modified by bacterial cellulose is the preparation method of carrier.

[0022] (1) Soak nano-titanium dioxide in 50-100mM, pH 5.0-6.0 phosphate buffer solution, ultrasonically clean, and then vacuum freeze-dry;

[0023] (2) Soak 1-100g of nano-titanium dioxide obtained in step (1) in 100-5000mL of phosphate buffer solution containing 1.0%-10% (w / w) bacterial cellulose, shake at 10-40°C for 1-100h, vacuum After suction filtration, wash with distilled water to obtain bacterial cellulose modified titanium dioxide carrier.

Embodiment 3

[0024] Embodiment 3: the preparation method of denitrifying bacteria bioball.

[0025] The TiO modified by denitrifying bacteria ATCC 14581 is fixed on the bacterial cellulose The method for the surface of the carrier is as follows: the bacterial cellulose modified TiO 2 The carrier is added to the culture medium of denitrifying bacteria ATCC 14581 to obtain shaking culture at 30-37° C. and 170-200 rpm for 1-8 hours to obtain denitrifying bacteria biospheres.

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Abstract

The invention discloses a denitrification bacterium biological sphere for a hairy crab breeding water purification system. The denitrification bacterium biological sphere is characterized in that bacterial cellulose modified TiO2 is adopted as a carrier, a denitrification bacterium is immobilized on the surface, and the denitrification bacterium is ATCC 14581. Purification of hairy crab breeding wastewater is simulated by using the biological sphere, the ammonium-nitrogen removal rate is up to 63% or greater, the CODCr (Chemical Oxygen Demand) removal rate is up to 71%, hairy crab breeding water purification requirements can be met, and a base can be made for large-scale artificial breeding of hairy crabs.

Description

technical field [0001] The invention belongs to the technical field of hairy crab artificial cultivation, and in particular relates to a nitrifying bacteria biosphere used in a water purification system for hairy crab cultivation and a preparation method thereof. Background technique [0002] Seine culture in natural lakes or pond culture is currently the main method of crab culture in China, and it is widely used. However, this breeding method has higher requirements on the water body environment, such as being easily affected by climate disasters and natural enemies. Moreover, it is difficult to track the status of crabs during the breeding process, and it is difficult to supervise, so that the survival rate and the quality of crab products cannot be well guaranteed. At the same time, long-term breeding will also cause serious pollution to natural water bodies, including artificial feed and hormones put in as crab food, residual bait feces of crabs or ammonia nitrogen, ni...

Claims

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Application Information

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IPC IPC(8): C12N11/12C12N11/14C02F3/34C12R1/01C02F103/20
CPCC02F3/34C02F2103/20C12N11/12C12N11/14
Inventor 浦育
Owner 苏州市阳澄湖蟹王水产有限公司
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