A kind of bone marrow mesenchymal stem cell storage solution

A technology for stem cell storage and bone marrow mesenchyme, applied in the field of bone marrow mesenchymal stem cell storage solution, to avoid damage, enhance maintenance effect, and simple formula

Active Publication Date: 2018-08-24
恩大细胞基因工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Ginsenoside RZ has not been seen so far 1 or RG 5 Reports used to preserve BMSCs to maximize their viability

Method used

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  • A kind of bone marrow mesenchymal stem cell storage solution
  • A kind of bone marrow mesenchymal stem cell storage solution
  • A kind of bone marrow mesenchymal stem cell storage solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Effects on the Survival Rate of Bone Marrow Mesenchymal Stem Cells

[0021] 1. Experimental method

[0022] 1. Preparation of stem cell storage solution

[0023] Prepare 0.9% normal saline (control group), ginsenoside RZ 1 Group (0.9% normal saline+30mM ginsenoside RZ 1 ), Ginsenoside RK 1 Group (0.9% normal saline+30mM ginsenoside RK 1 ), Ginsenoside RG 5 Group (0.9% normal saline+30mM ginsenoside RG 5 ), Ginsenoside RZ 1 +RG 5 Group (0.9% normal saline+30mM ginsenoside RZ 1 +10mM Ginsenoside RG 5 ), Ginsenoside RZ 1 、RK 1 , RG 5 Dissolve directly in 0.9% normal saline and shake well.

[0024] 2. Isolation and culture of BMSCs

[0025] C57BL / 6 mice at 6-8 weeks were sacrificed by cervical dislocation, and femurs and tibias were taken, the bone marrow cavity was washed with PBS, blown into a single cell suspension, filtered, centrifuged (1500r / min, 5min), and the supernatant was removed. Add 5ml of erythrocyte lysate, lyse for 5-6min, add an ...

Embodiment 2

[0033] Example 2: The effect of BMSCs stored for 12h on the regeneration of myelin in rats with spinal cord injury

[0034] 1. Experimental method

[0035] 1. Preparation of spinal cord injury rat model

[0036] After adult rats (body weight 250-280 g) were anesthetized, the spinous processes and lamina of T9-T11 were exposed and removed to fully expose the spinal cord at the end of T9-T11. Be careful not to damage the dura mater. The spinal cord was hit with 10.0g, 0.45mm / s, and the spasmodic swing of the rat tail and paralysis of both lower limbs indicated that the model was successfully prepared. Penicillin (10 mg / kg) was given within 7 days after operation to prevent infection. Artificial voiding was performed twice daily until spontaneous voiding resumed.

[0037] 2. Orthotopic transplantation of bone marrow mesenchymal stem cells

[0038] The BMSCs stored in different storage solutions for 12 hours in Example 1 were orthotopically transplanted into model rats with...

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Abstract

The invention relates to storage liquid for mesenchymal stem cells. The storage liquid is characterized in that a solution is 0.9% normal saline and the solution further contains effective-concentration ginsenoside RZ1. The storage liquid has the advantages that the survival rate of the mesenchymal stem cells can be maintained to the maximum degree, the survival rate lower percentage after 12 hours of storage can be controlled to be within 10%, and the storage liquid evidently better than the 0.9% normal saline which does not contain the ginsenoside RZ1; in addition, if effective-concentration ginsenoside RG5 is also added, the survival rate lower percentage after 12 hours of storage can be controlled to be within 5%; accordingly, the ginsenoside RZ1 can be used for preparing the storage liquid for the mesenchymal stem cells, and the ginsenoside RG5 can further enhance the maintaining effect of the ginsenoside RZ1 on the survival rate of the mesenchymal stem cells.

Description

technical field [0001] The invention relates to stem cell storage, in particular to a bone marrow mesenchymal stem cell storage solution. Background technique [0002] Bone marrow mesenchymal stem cells (BMSCs) transplantation can be used for the treatment of spinal cord injury and other diseases. After transplantation of bone marrow mesenchymal stem cells to the injured spinal cord, it was found that BMSCs can promote the regeneration of axons and the recovery of motor function at the injured site. [0003] Before transplantation, BMSCs need to be collected from the expanded culture flask, and then use non-medium solution to make cell suspension for transplantation. When conducting animal model experiments, we will transplant the resuspended stem cells in the shortest possible time. However, it is not feasible to transplant the resuspended cells immediately in clinical treatment. Cell collection, surgery, and long-distance transport of stem cells can all increase the wait...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 张丹丹杨勇胡志香
Owner 恩大细胞基因工程有限公司
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