Euphorbia esula total flavonoid extract as well as preparation method and antitumor activity application thereof

A technology of total flavonoids and extracts, which is applied in the field of anti-tumor and anti-tumor auxiliary new drugs, extracts of total flavonoids of milk sorrel and its preparation, to achieve the effect of inhibiting tumor growth and improving competitiveness

Inactive Publication Date: 2017-12-08
JILIN ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are very few literature reports on the analysis and research of the components of this medicinal material. Some scholars have isolated 3 diterpene compounds from Euphorbia japonicus [1] ; Quercetin-3-O-α-L-rhamnoside, kaempferol-3-O-β-D-glucopyranoside, quercetin-7- O-β-D-glucopyranoside three flavonoid monomer compounds [2]

Method used

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  • Euphorbia esula total flavonoid extract as well as preparation method and antitumor activity application thereof
  • Euphorbia esula total flavonoid extract as well as preparation method and antitumor activity application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] (1) Take 3kg of milky herbal medicine (dry part of Euphorbia euphorbia above the ground), chop it into pieces, add 24kg of water to decoct and extract twice, each time for 2 hours.

[0022] (2) Combine the filtrates, filter while hot, concentrate to a volume of 1L, add to the treated D 101 A macroporous resin column with a column bed volume of 1 L was first eluted with 8 L of water and then 8 L of 95% ethanol. The eluate was concentrated and dried after recovering the ethanol to obtain 117 g of the crude extract of total flavonoids of Milkwort.

[0023] (3) Take the crude extract, stir it with 936ml of 40% ethanol for 30 minutes, filter, concentrate the filtrate in a water bath to a thick paste, dry it under reduced pressure, and make 73g of total flavonoids extract of Milkwort. The total flavonoid content of the prepared milk sorrel is 86%.

Embodiment 2

[0025] (1) Take 3kg of milky herbal medicine (dry part of Euphorbia euphorbia above the ground), chop it into pieces, add 18kg of water to decoct and extract 3 times, 1 hour each time.

[0026] (2) Combine the filtrates, filter while hot, concentrate to a volume of 1L, add to the treated AB-8 macroporous resin column, the volume of the column bed is 1L, first wash with 6L of water, and then wash with 6L of 30% ethanol Ethanol was recovered from the eluent, concentrated and dried to obtain 127 g of the crude extract of total flavonoids of Latex sorrel.

[0027] (3) Take the crude extract, stir it with 762ml of 75% ethanol for 20 minutes, filter, concentrate the filtrate in a water bath to a thick paste, dry under reduced pressure, and make 76g of total flavonoids extract of Milkwort. The total flavonoid content of the prepared milk sorrel is 52%.

Embodiment 3

[0029] (1) Take 3kg of milky herbal medicine (dried part of Euphorbia euphorbia above the ground), chop it into pieces, add 27kg of water to decoct and extract twice, 1.5 hours each time.

[0030] (2) Combine the filtrates, filter while hot, concentrate to a volume of 1L, add to the treated D 21 A macroporous resin column with a column bed volume of 1L, first eluted with 10L of water, and then eluted with 10L of 70% ethanol, and the eluate was concentrated and dried after recovering the ethanol to obtain 144g of the crude extract of total flavonoids of Milk Sorrel.

[0031] (3) Take the crude extract, stir it with 1440ml of 50% ethanol for 10 minutes, filter, concentrate the filtrate in a water bath to a thick paste, dry under reduced pressure, and make 52g of total flavonoids extract of Milkwort. The total flavonoid content of the prepared milk sorrel is 67%.

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Abstract

The invention relates to a euphorbia esula total flavonoid extract as well as a preparation method and an antitumor activity application thereof and belongs to the technical field of pharmacy. New anti-tumor drugs and new anti-tumor auxiliary drugs which are prepared by taking the euphorbia esula total flavonoid extract as a raw material can effectively inhibit tumor growth, and an application of treating a tumor is achieved. The euphorbia esula total flavonoid extract provided by the invention has the beneficial effects that a gap in preparation of the euphorbia esula total flavonoid extract is filled up, application range of the euphorbia esula total flavonoid extract in the field of preparation of the anti-tumor drugs and the anti-tumor auxiliary drugs is also expanded, so that competitiveness of the preparation in the international market is improved.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and relates to an extract of total flavonoids of milk sorrel and a preparation method thereof, and the application of anti-tumor and anti-tumor auxiliary new drugs prepared from the extract, which can effectively inhibit tumor growth and achieve the purpose of treating tumors. Background technique [0002] Cancer is a common and frequently-occurring disease that seriously threatens human life and health. It is a worldwide intractable disease. The death rate of cancer ranks first among all causes of death in many countries. Clinically, traditional chemotherapy drugs are cytotoxic drugs. While killing tumor cells, cytotoxic drugs also destroy normal cells in the human body. Their selectivity is low and their toxicity is high. Long-term drug use can cause serious late complications, that is, secondary tumors. Occurrence, especially severe toxic side effects such as acute non-lymphocytic leukemia an...

Claims

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Application Information

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IPC IPC(8): A61K36/47A61P35/00
CPCA61K36/47A61K2236/331A61K2236/39A61K2236/51A61K2236/55
Inventor王隶书程东岩赵宏宇陈昕程东红高军刘晓杰高佳王超楠
OwnerJILIN ACAD OF TRADITIONAL CHINESE MEDICINE