Avian influenza virus H7N9 sub type fluorescent RT-PCR primer group, probe group, kit and method
An avian influenza virus, RT-PCR technology, applied in the field of avian influenza virus H7N9 subtype fluorescent RT-PCR primer set, can solve the problems of difficult double amplification specificity, sensitivity control, etc., to achieve good repeatability, impurity little effect
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Embodiment 1
[0059] A kind of avian influenza virus H7N9 subtype fluorescent RT-PCR primer set, comprises following primers:
[0060] H7-F: 5'-TCACAGCAAATACAGGGAAGAG-3';
[0061] H7-R: 5'-CCCGAAGCTAAACCAGAGTATC-3';
[0062] N9-F: 5'-TAGCAATGACACACACTAGTCAAT-3';
[0063] N9-R: 5'-ATTACCTGGATAAGGGTCATTACACT-3';
[0064] HA-F: 5'-CTTCTAACCGAGGTCGAAACGTA-3';
[0065] HA-R: 5'-GGTGACAGGATTGGTCTTGTCTTTA-3'.
Embodiment 2
[0067] A kind of avian influenza virus H7N9 subtype fluorescent RT-PCR probe set, comprises following probe:
[0068] H7-P5: 5'-ROX-TGACCCAGTCAAACTAAGCAGCGG-MGB-3';
[0069] N9-p7: 5'-JOE-AGACAATCCCCGACCGAATGACCC-MGB-3';
[0070] HA-P2: 5'-CY5-TCAGGCCCCCTCAAAGCCGAG-MGB-3'.
Embodiment 3
[0072] A kind of avian influenza virus H7N9 subtype fluorescent RT-PCR kit, comprises the primer group described in embodiment 1 and the probe group described in embodiment 2.
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