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Screening method and application of mitochondria-nucleus DNA methylation joint sites

A methylation and mitochondrial technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as lack of breast cancer risk prediction, and achieve the effect of accurate screening criteria

Active Publication Date: 2021-02-02
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at this stage, breast cancer risk prediction lacks a convenient, quick, non-invasive, and reliable method, and there are no related technologies and products on the market that use changes in the methylation level of specific sites to achieve early breast cancer risk prediction and screening

Method used

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  • Screening method and application of mitochondria-nucleus DNA methylation joint sites
  • Screening method and application of mitochondria-nucleus DNA methylation joint sites
  • Screening method and application of mitochondria-nucleus DNA methylation joint sites

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Embodiment approach

[0023] According to a typical embodiment of the present invention, a method for screening mitochondrial-nuclear DNA methylation joint sites is provided. The screening method comprises the following steps: a) obtaining patient samples and healthy samples; b) extracting mitochondrial DNA and nuclear DNA from patient samples and healthy samples; c) determining the mitochondrial DNA and nuclear DNA methylation levels of patient samples and healthy samples d) analyzing the methylation information obtained in step c), and screening methylation sites with significant differences between patient samples and healthy samples; and e) joint analysis of mitochondrial DNA and nuclear DNA methylation, screening to obtain risk prediction sites point.

[0024] Among them, the combined analysis can be carried out by conventional analysis methods in the field, for example, by randomly combining the methylation sites of mitochondrial DNA and nuclear DNA, comparing the degree of differentiation be...

Embodiment 1

[0037] In this example, by comparing the blood samples of breast cancer patients and their healthy family members in five families (15 people in total, each family contains three generations of maternity, and one person is diagnosed as a breast cancer patient), the family specificity is found. It was found that mitochondrial DNA methylation follows certain maternal inheritance rules; at the same time, specific differential methylation sites were found, that is, methylation sites with significant differences between breast cancer patients and healthy members , and take the average methylation level of these sites in breast cancer patients as the verification standard, compare the methylation level of this site of all the subjects to be tested, and observe whether there is any abnormality, so as to judge whether the person to be tested has breast cancer cancer risk.

[0038] Specifically, the methylation sites with significant differences in mitochondrial DNA and nuclear DNA wer...

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Abstract

The invention discloses a screening method and application of mitochondria-nucleus DNA methylation joint sites. Wherein, the screening method comprises the following steps: a) obtaining patient samples and healthy samples; b) extracting mitochondrial DNA and nuclear DNA from patient samples and healthy samples; c) determining the mitochondrial DNA and nuclear DNA methyl groups of patient samples and healthy samples d) analyze the methylation information obtained in step c), and screen the methylation sites with significant differences between patient samples and healthy samples; and e) joint analysis of mitochondrial DNA and nuclear DNA methylation, screen out the risk predicted location. Applying the technical solution of the present invention, it is possible to effectively screen cancer risk prediction markers, provide more accurate screening criteria, expand the approach to clinical tumor detection, and the screened specific sites can be effectively used for cancer risk predict.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a screening method and application of mitochondria-nucleus DNA methylation joint sites. Background technique [0002] DNA methylation is a common epigenetic modification. Under the catalysis of DNA methyltransferase, the methyl group provided by S-adenosylmethionine is used to form the fifth carbon atom of cytosine. Cytosine converts cytosine into 5-methylcytosine, which plays a vital role in the regulation of gene expression. [0003] Tumor is a malignant disease caused by the abnormality of the mechanism of controlling cell growth and proliferation. Under normal circumstances, the growth and proliferation of cells are tightly regulated by some genes that regulate growth and development, including oncogenes and tumor suppressor genes, and maintain a normal physiological state; under various factors such as radiation, chemical pollution, virus infection, and endocrine imbalance Und...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
Inventor 孙英丽韩潇
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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