Tissue shearing method and device, application of shearing method

Abstract

Disclosed is a tissue shearing method. The tissue shearing method comprises the steps that firstly, tissue to be sheared is placed into a container, then a fixed knife and a movable knife are placed in the container, and then the container is closed; and acting force is applied on one end of the movable knife, so that the movable knife rotates around a pivot of the fixed knife, a cutting part of the movable knife and a cutting part of the fixed knife do relative movement of opening and closing, and the tissue is cut into fragments with the sizes being 1 mm<3>-2 mm<3>. Compared with the mode ofrealizing smashing by hand-holding a surgical scissors for conducting repeated shearing on the tissue, the tissue shearing method can realize tissue shearing in an automatic or semi-automatic way andis suitable for tissue shearing of the preparation process of placenta and umbilical cord mesenchymal stem cells to prepare primary cells. In the process of cutting into pieces, the tissue temperature is increased by within 2 DEG C, and cell activity can be maintained.

Description

technical field [0001] The present invention relates to a crushing method, in particular to a method for cutting tissue, and a device for cutting tissue, especially tissue such as umbilical cord and placenta, so that collagenase can be used for digestion . Background technique [0002] As of September 2017, the number of global mesenchymal stem cell clinical studies registered on the website www.clinicaltrials.gov in the United States has reached 759, and perinatal mesenchymal stem cells account for a large proportion of them. The extensive research and application of mesenchymal stem cells has also led to the construction and development of mesenchymal stem cell banks. Taking the preparation of placental mesenchymal stem cells as an example, the cutting process mainly includes the following steps: separate and wash the placental chorionic tissue, and cut it into 1mm 3 ~2mm 3 Size fragments, collagenase digestion and centrifugation, cell count and seed culture. Among the...

AI Technical Summary

Problems solved by technology

The preparation of a sample often requires continuous shearing for 5 to 10 minutes to reach the tissue size requirement for digestion, which is the most laborious link in the entire preparation process

In addition, currently known commonly used automated tissue crushing equipment, such as: electric hand-held tissue homogenizer, and a homogenizer driven by a motor (CN205576112U), its advantage is that it can mash the tissue quickly and labor-savingly, and the disadvantage is that the homogenizer High-speed operation causes the temperature of the tissue center to rise rapidly, and the particle size of the tissue cannot be controlled. The tissue mashed by the homogenizer is usually in a homogenized state, which will destroy the integrity and activity of primary cells or stem cells, so it is not suitable for preparation Fragile and precious primary or stem cells

In addition, the tissue shearing devices reported in Chinese utility model patents CN205817926U and CN206154357U all continue the traditional way of inserting fingers into rings for cutting, which cannot fundamentally change the soreness of fingers or wrists caused by long-term work by technicians. status

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