Rapid nucleic acid amplification system

Abstract

The invention provides a rapid nucleic acid amplification system. Transfer of a nucleic acid amplification reaction solution in a reaction consumable among different temperature zones is performed bya rotating mode and rapid amplification of nucleic acid is realized; the technical problem that an existing nucleic acid amplification system is long in consumed time or the reaction consumable is difficult to realize is solved. The system provided by the invention comprises a disk-shaped or sector reaction consumable, a temperature control module and a rotary supporting assembly. A PCR (Polymerase Chain Reaction) reaction area close to the edge is arranged on the reaction consumable; the temperature control module comprises two or more groups of circumferentially-distributed PCR temperature zone control units and is used for controlling the temperature of the PCR reaction area on the reaction consumable. The reaction consumable is arranged on a supporting component of the rotary supporting assembly; the PCR reaction area is controlled by a rotary motor to transfer between different temperature zone control units and the rapid amplification of the nucleic acid is realized.

Description

technical field

[0001] The invention relates to the fields of molecular biology and molecular diagnosis, in particular to a system for rapidly realizing nucleic acid amplification. Background technique

[0002] Nucleic acid detection is a basic experimental method in molecular biology. Molecular diagnosis is a technology that uses molecular biology methods to detect exogenous or self-genetic material "nucleic acid" and its expression level in patients' bodies or body fluids to make a diagnosis. It is one of the important frontier areas of contemporary medical development. The basis of molecular diagnosis is the detection and analysis of target nucleic acids. Molecular diagnostic technologies mainly include real-time fluorescence quantitative PCR, fluorescence in situ hybridization (FISH), gene sequencing and gene chips, etc. Molecular diagnostic techniques basically include three steps: nucleic acid extraction and purification, nucleic acid amplification and detection. Nuc...

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