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Construction and application of medicine screening model on the basis of PPAR (Peroxisome proliferator-activated receptors) gamma signal channel

A technology of selection and base, applied in the field of biomedicine, can solve the problems of complicated operation and few clinical applications.

Inactive Publication Date: 2018-03-09
上海米络凯生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Antagonists of PPARγ can theoretically inhibit the generation of adipocytes and prevent obesity, but there are few clinical applications at present
[0004] So far, domestic high-throughput drug screening methods for PPARγ agonists and antagonists are mostly limited to small molecule compound screening at the protein level, such as SPR technology, which is cumbersome to operate

Method used

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  • Construction and application of medicine screening model on the basis of PPAR (Peroxisome proliferator-activated receptors) gamma signal channel
  • Construction and application of medicine screening model on the basis of PPAR (Peroxisome proliferator-activated receptors) gamma signal channel
  • Construction and application of medicine screening model on the basis of PPAR (Peroxisome proliferator-activated receptors) gamma signal channel

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Embodiment Construction

[0044] The purpose of the present invention is to construct a drug screening cell model based on PPARγ signaling pathway.

[0045] In the construction of the cell model, the present invention uses a polynucleotide sequence, which contains the PPARγ-specific binding sequence AGGTCA. Preferably, the polynucleotide sequence consists of a plurality of AGGTCAs, a linker sequence and optionally cohesive ends. Specifically, the number of repeats of the binding sequence can be 2-100, and the linker sequence between every two binding sequences can be a polynucleotide sequence with a length of 1-25 bases, and the linker sequences can be the same or different. The invention also includes the complements of said polynucleotide sequences.

[0046] Preferably, the number of repeats of the binding sequence is 2-50, more preferably 5-40, more preferably 10-35. The cohesive end can be set according to the endonuclease used, and its length and composition vary depending on the endonuclease used...

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Abstract

The invention relates to construction and application of a medicine screening model on the basis of a PPAR (Peroxisome proliferator-activated receptors) gamma signal channel. Specifically, the invention provides a polynucleotide sequence which is selected from N1-(AGGTCA-N-AGGTCA)m-N2 and the complementary sequence thereof, wherein N1, N, N2 and m are disclosed in the invention. The invention alsoprovides a reporter vector which contains the polynucleotide sequence and a luciferase reporter gene, a kit which contains the reporter vector, a cell in which the reporter vector is transferred, a preparation method of the cell and the application of the polynucleotide, the kit and the cell. The screening system is stable, is convenient in operation and is more suitable for screening high-flux medicines, and a screening period is shortened.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the construction and application of a drug screening model based on PPARγ signaling pathway. Background technique [0002] In recent years, with the improvement of living standards, people's dietary structure and habits are constantly changing, and the resulting hypertension, hyperlipidemia, diabetes, atherosclerosis, coronary heart disease, obesity and cancer are also plagued by more and more people. More and more people, at the same time with the continuous deepening of scientific research, also found that the correlation of the above diseases is very close. PPARs (Peroxisome proliferator-activated receptors) are members of the nuclear receptor transcription factor superfamily that regulates the expression of target genes (Zhu, Kan et al., 2000). According to different structures, PPARs can be divided into three subtypes, PPARα, β, and γ (Berger and Moller, 2002). It has been found th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/867C12N5/10C12Q1/02
CPCC07K14/4702C12N5/067C12N15/86C12N2503/02C12N2510/00C12N2740/15043C12N2800/107G01N33/5041G01N2500/10
Inventor 杨金波
Owner 上海米络凯生物科技有限公司
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