The invention relates to the field of
molecular genetics, in particular to the application of a
molecular marker method in
pig breeding for
disease resistance, wherein according to the
molecular marker method, the molecule at a
mutation site in a CYP3A88
gene 5'
regulatory region of a pig is marked. The inventor of the method discovers that the CYP3A885'
regulatory region of a large Chinese streaky-head pig and the CYP3A885'
regulatory region of a Duroc long
hybrid pig have a plurality of differences, wherein an A-to-T
mutation exists at the -78 site, through a
luciferase reporter gene system, the fact that
promoter activity of the -78 site is lowered remarkably after an A at the -78 site is mutated into a T in
a site-
directed mutagenesis mode is found, and the
promoter activity of the -78 site is the same as the low level of
messenger ribonucleic acid (mRNA) expression of a CYP3A88
gene of the porcine reproductive and respiratory syndrome (PRRS)-resistant large Chinese streaky-head pig. Therefore, through
genotype detection of the -78 site of the CYP3A88 regulatory region in a pig
genome, the
genotype of the -78 site of the CYP3A88 regulatory region can be used as a modular marker associated with traits of the PPRS, the
molecular marker method not only is simple, convenient and rapid, but also cannot be affected by the environment, and early selection for breeding can be realized.