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Magnetic particle chemiluminescence detection kit for free beta human chorionic gonadotropin (free beta-hCG) and preparation method thereof

A chorionic gonadotropic and chemiluminescence detection technology, which is applied in chemiluminescence/bioluminescence, biological testing, and analysis through chemical reactions of materials, can solve the problems of short validity period of reagents, narrow detection range, and low sensitivity. Achieve the effects of good reagent stability, low background luminescence, and high signal-to-noise ratio

Inactive Publication Date: 2018-03-13
太原瑞盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to provide a free β-hCG chemiluminescent quantitative detection kit and its preparation method, which avoids the short validity period of the reagents for radioimmunoassay, radioactive contamination, and operation It is cumbersome to determine, and solves the defects of low sensitivity, narrow detection range and high cost

Method used

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Examples

Experimental program
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preparation example Construction

[0021] The present invention provides a magnetic particle chemiluminescence detection kit and preparation method for free β-human chorionic gonadotropin. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below.

[0022] The present invention provides a magnetic particle chemiluminescent detection kit for free β-human chorionic gonadotropin and a preparation method thereof, wherein the magnetic particle chemiluminescence method provided by the present invention detects free β-human chorionic gonadotropin, Using magnetic particle coupling free β human chorionic gonadotropin capture antibody, acridinium ester labeled free β human chorionic gonadotropin detection antibody. The kit also includes free β-human chorionic gonadotropin calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution for the above-mentioned a...

Embodiment 1

[0033] Embodiment 1: The formation and preparation method of a magnetic particle chemiluminescence kit for detecting free β-human chorionic gonadotropin includes the following steps:

[0034] 1. The composition of the kit:

[0035] A magnetic particle chemiluminescent kit for detecting free β-human chorionic gonadotropin was constructed so that it contained the following components:

[0036] Monoclonal antibody to free β-human chorionic gonadotropin coupled to magnetic particles;

[0037] Another free β-human chorionic gonadotropin monoclonal antibody labeled with acridinium ester;

[0038] Free β-human chorionic gonadotropin series standard solution;

[0039] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0040] Cleaning fluid.

[0041] 2. Preparation of magnetic particle suspension conjugated with free β-human chorionic gonadotropin monoclonal antibody

[0042] (1) Take 1 mg of carboxyl magnetic particles in a 0.5 mL centrifug...

Embodiment 2

[0062] Example 2: The steps for detection using the magnetic particle chemiluminescence detection kit for free β-human chorionic gonadotropin are:

[0063] (1) Add 50 μL of the sample to be tested into the cuvette, then add 150 μL of magnetic particle coupling suspension, shake and mix, and incubate at 37°C for 8 min.

[0064] (2) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0065] (3) Add 150 μL of acridinium ester marker into the cuvette, shake to mix, and incubate at 37°C for 7 min.

[0066] (4) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0067] (5) Add 100 μL of chemiluminescence pre-excitation solution A, add 100 μL of chemiluminescence excitation solution B after 1 s, and measure its relative luminous intensity. The content of free β-human chorionic gonadotropin in the sample is proportional to its relative luminous intensity relation.

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Abstract

The invention discloses a magnetic particle chemiluminescence detection kit for free beta-hCG and a preparation method of the magnetic particle chemiluminescence detection kit for free beta-hCG. The kit comprises magnetic particles coupled with free beta-hCG capture antibodies, acridinium ester labeled free beta-hCG detection antibodies, free beta-hCG calibrators, chemiluminiscence pre-excited liquid A, chemiluminescence excitation liquid B and cleaning liquid. The kit disclosed by the invention uses magnetic separation chemiluminescence technologies as a detection means, and further combineswith acridiniumester labeling technologies. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and high speed, short detection time, higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

Description

technical field [0001] The invention belongs to the technical field of immunodetection and analysis, in particular to an immunomagnetic particle chemiluminescence detection kit and a preparation method of free beta human chorionic gonadotropin. Background technique [0002] Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted by trophoblast cells of the placenta, which is composed of two subunits α and β in a non-covalent bond. Glycoprotein hormone with a molecular weight of 36700. hCG is produced by the placenta and cytotrophoblast cells, and its α-subunit shares the same amino acid series as the α-subunit of other hormones in the glycoprotein hormone family (eg, LH, FSH, and TSH), while the β-subunit differs. hCG exists in various forms in human body fluids, such as intact form (hCG), free α subunit (hCGα), free β subunit (hCGβn), incomplete hCG (nicked hCG, hCGn), incomplete free hCGβ subunit Unit (hCGβn), and free hCGβ subunit core fragment (Core fragm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/543G01N33/577G01N33/76
CPCG01N33/76G01N21/76G01N33/54326G01N33/577G01N2333/59
Inventor 程月萍李瑶许殊荣杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
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