38results about How to "Yield does not decrease" patented technology

The invention relates to a method for separating agricus bisporus polysaccharides from agricus bisporus, which comprises the following steps: carrying out vacuum freeze-drying on the agricus bisporus for 24h; extracting crude polysaccharides with hot water: adding distilled water, boiling with big fire, maintaining with slow fire, centrifuging, taking supernatant liquid, precipitating, placing back into a heating device, adding water for continuous heating till boiling, maintaining with the slow fire, re-centrifuging and taking the supernatant liquid; repeating for three times, and using the distilled water for fixing volume of the obtained supernatant liquid for standby; carrying out dual-water phase extraction operation: precisely weighing 2.66g of polyethylene glycol (PEG) 6000 and 4.28g of ammonium sulfate, adding into a 50mL centrifuge tube, adding 20mL of sample diluent, and evenly mixing; simultaneously weighing 2.66g of PEG 6000 and 4.28g of ammonium sulfate, adding into the 50mL centrifuge tube, adding 20mL of distilled water, evenly mixing, and obtaining the agricus bisporus polysaccharides distributed in the PEG phase. The utilization of a dual-water phase system for extracting the agricus bisporus polysaccharides can realize the maximum yield of 80.1%, and the yield can achieve 17.45mg / g.

The invention discloses a calcitonin magnetic particle chemiluminescent detection kit and a preparation method. The kit includes: magnetic particles coupled with calcitonin capture antibody, acridinium ester-labeled calcitonin detection antibody, calcitonin calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and rapidity, short detection time and higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

The invention discloses a magnetic particle chemiluminescence detection kit for placental growth factor and a preparation method. The kit includes: magnetic particles coupled with placental growth factor capture antibody, acridinium ester-labeled placental growth factor detection antibody, placental growth factor calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses magnetic separation chemiluminescence technology as a detection method and combines it with acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and rapidity, short detection time, and higher accuracy and repeatability of detection results. The kit can be applied to various luminescence detection instruments.

The invention discloses a preparation method of (S)-1-(2,6-dichloro-3-fluorophenyl) ethanol. The preparation method comprises the following steps: by taking 2,6-dichloro-3-fluoroacetophenone as a starting raw material, reducing the 2,6-dichloro-3-fluoroacetophenone by use of sodium borohydride and then reacting the 2,6-dichloro-3-fluoroacetophenone with phthalic anhydride to obtain a half-ester, next, carrying out resolution by use of s-methylbenzylamine and then carrying out deprotection by hydrolyzing to remove phthalic acid, thereby obtaining the high-optical purity target product. The preparation method is simpler and more reasonable in synthesis process and convenient and feasible in after-treatment; the intermediate can be directly applied to next step of reaction without being purified, so that the after-treatment process can be reduced; meanwhile, the adopted resolution agent and the phthalic acid both can be recovered; as a result, the whole process is suitable for large-scale industrial production.

The invention discloses a synthetic method of biphenyl compounds which is prepared through adding alkalis, alcohol and halogenated aromatic compounds into water, mixing it to form microemulsion system, then adding Pd / C as catalyst, reacting 2 to 10h at 50 to 120 DEG C, postprocessing the resultant of reaction to obtain the biphenyl compounds. The present invention is characterized in that using alcohol as additive agent, using lower price Pd catalyst, having features of lower cost, short reaction time, high yield, no pollution and in favor of industrialization.

The invention discloses a magnetic particle chemiluminescence detection kit for free beta-hCG and a preparation method of the magnetic particle chemiluminescence detection kit for free beta-hCG. The kit comprises magnetic particles coupled with free beta-hCG capture antibodies, acridinium ester labeled free beta-hCG detection antibodies, free beta-hCG calibrators, chemiluminiscence pre-excited liquid A, chemiluminescence excitation liquid B and cleaning liquid. The kit disclosed by the invention uses magnetic separation chemiluminescence technologies as a detection means, and further combineswith acridiniumester labeling technologies. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and high speed, short detection time, higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

The present invention relates to 2, 2'-biquinoline synthesizing process. Inside water and polyethylene glycol (PEG) solvent system and in the presence of Pd / C catalyst, quinoline material is reacted at 140 deg.c for 4-20 hr to obtain 2, 2'-biquinoline. In the reaction system, the weight ratio between PEG and water is 0.2-2 to 1, the Pd / C catalyst has Pd supporting amount of 1-10 wt%, the PEG has molecular weight of 400-6000, the weight ratio between the Pd / C catalyst and quinoline is 0.1-1 to 1, and the weight ratio between the water / PED solvent system and quinoline is 3-10 to 1. The present invention has low material cost, simple operation, high yield and less environmental pollution, and is suitable for industrial production.

The invention discloses a tetracycline chemiluminescence detecting kit and a preparation method thereof. The kit comprises an acridinium ester marked tetracycline antigen or antibody, a magnetic particle coupled antigen or antibody, a tetracycline series standard substance solution, a chemiluminescence pre-excited solution A, a chemiluminescence pre-excited solution B and a cleanout fluid. The kit integrates a chemiluminescence technology and immune magnetic particles, and a reaction system approximate to a homogeneous phase is provided. Compared with the prior art, the kit has the advantages of high sensitivity, strong specificity, short reaction time and the like.

The invention discloses a magnetic particle chemiluminiscence detection kit for a vascular endothelial growth factor receptor 1 and a preparation method. The kit comprises vascular endothelial growthfactor receptor 1 capture antibody coupled magnetic particles, an acridinium ester marked vascular endothelial growth factor receptor 1 detecting antibody, a vascular endothelial growth factor receptor 1 calibrator, chemiluminiscence pre-excitation liquid A, chemiluminiscence pre-excitation liquid B and cleaning liquid. The kit adopts a magnetic separation chemiluminiscence technology as a detection means combined with an acridinium ester marking technology. A direct chemiluminiscence method is high in sensitivity and specificity, accurate, quick and short in detection time, detection resultsare high in accuracy and repeatability, and the kit is applicable to various luminescence detection instruments.

The invention discloses a magnetic particle chemiluminescence detection kit for human soluble ST2 and a preparation method. The kit comprises magnetic particles coupled with a soluble ST2 capture antibody, an acridinium ester-labeled soluble ST2 detection antibody, a soluble ST2 calibrator, a chemiluminescent pre-excitation solution A, a chemiluminescent excitation solution B, and a cleaning solution. The kit disclosed by the invention adopts magnetic separation chemiluminescence technology as a detection means, and simultaneously combines acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, high specificity, accuracy and rapidity, and short detection time, the detection result has higher accuracy and repeatability, and the kit can be suitable for various illumination detecting instruments.

The invention provides a reagent for detecting COVID-19 through chemiluminescence immunoassay and a detection method thereof. An indirect process is used, a COVID-19 antigen is coated on a magnetic microsphere, the anti-human IgG / IgM is labeled with acridinium ester, and the acridinium ester is excited by the excitation liquid to emit light so as to detect the COVID-19 antibody, and the detectionmethod combining the acridinium ester or acridinium sulfonamide compound labeling technology and the superparamagnetic nano-microsphere labeling technology is adopted; the superparamagnetic nano-microsphere labeling technology is a technology of coating nano-microspheres with antigens or antibodies, and the superparamagnetic nano-microsphere labeling technology can enlarge the reaction surface area by 10 to 50 times and accelerate the reaction efficiency; the reaction process is suspended in a reaction solution and belongs to homogeneous reaction, so that the reaction speed can be increased; connection belongs to chemical connection of bioactive substances, so that the coating efficiency is higher, and the coating is more stable in a liquid environment. The detection reagent disclosed by the invention is high in specificity, high in sensitivity, simple and convenient to separate, rapid, non-toxic, safe and stable, short in detection time by adopting the reagent, high in efficiency andgood in stability.

The invention discloses a chemiluminescence detection kit of aureomycin and a preparation method thereof. The kit comprises the following components: an acridinium ester labelled aureomycin antigen orantibody, a magnetic particle coupled antibody or antigen, an aureomycin series standard substance solution, chemiluminescence preexcitation liquid A, chemiluminescence preexcitation liquid B, and cleaning fluid. The kit combines chemiluminescence technology and immunomagnetic particles, and provides a reaction system approaching to a homogeneous phase. Compared with the prior art, the kit has the advantages of high sensitivity, good specificity, short reaction time, and the like.

The invention relates to the technical field of microelectronics, and particularly relates to a resistive random access memory and a reset operation method thereof. The resistive random access memory comprises a control circuit, a writing voltage generation circuit and a current detection circuit, wherein the resistive random access memory is subjected to reset operation by adopting continuous ladder pulses. By adopting the resistive random access memory disclosed by the invention, no reduction of reset yield is ensured besides a verifying operation in the reset operation process, the reset operation time is also shortened, and the resistive random access memory has a good application value.

The invention discloses a magnetic particle chemiluminescent detection kit for pregnancy-related plasma protein A and a preparation method thereof. The kit includes: magnetic particles coupled with pregnancy-associated plasma protein A capture antibody, acridinium ester-labeled pregnancy-associated plasma protein A detection antibody, pregnancy-associated plasma protein A calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and rapidity, short detection time and higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

The invention discloses a salbutamol magnetic particle chemiluminescence detection kit and a preparation method. The kit includes: acridinium ester markers, magnetic particles coupled with antigens or antibodies, albuterol calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and speed, short detection time and higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

The invention relates to a preparation method for a gold nanometer catalyst. The preparation method comprises the following steps: dispersing a gold-source compound and a nanometer template in a solvent; adding alkali into a reaction product obtained in the previous step to adjust the pH value of the reaction product and carrying out heating so as to allow the reaction product to react with the alkali; and subjecting a product obtained in the previous step to filtering and subjecting the obtained filter cake to vacuum drying and then to calcining so as to obtain the gold nanometer catalyst. The invention also relates to a synthetic method for a quinazoline compound. The quinazoline compound is prepared by subjecting a hydrogen acceptor, a hydrogen donor, a nitrogen source and the gold nanometer catalyst to a reaction in an inert atmosphere, e.g., a nitrogen atmosphere. The gold nanometer catalyst provided by the invention has high efficiency, is repeatedly usable and can be used for catalytic preparation of the quinazoline compound.

The invention discloses a leptin chemiluminescent detection kit and a preparation method thereof. The kit comprises: magnetic particles coupled to a leptin capture antibody, a leptin detection antibody labelled by acridinium ester, a leptin calibration product, a chemiluminescent pre-triggered solution A, a chemiluminescent triggered solution B and a cleaning fluid. According to the kid, magneticseparation chemiluminescent technology is taken as a detection means, and is combined with acridinium ester labelling technology. The established direct chemiluminescent method is high in sensitivity,good in specificity, and short in detection time, and is accurate and fast. The detection result is high in accuracy and repeatability, and the kit is suitable for various luminescent detection apparatuses.

The invention discloses a neutral granulocyte elastase chemiluminescence detection kit and a preparation method thereof. The kit comprises a magnetic particle coupled neutral granulocyte elastase (NE)capture antibody, acridinium ester-labelled neutral granulocyte elastase (NE) detection antibody, a neutral granulocyte elastase calibration product, a chemiluminescence pre-excitation solution A, achemiluminescence pre-excitation solution B, and a cleaning solution. The kit combines a chemiluminescence technology and immunization magnetic particles, and provides a reaction system which approaches to a homogeneous phase. Compared with the prior art, the established direct chemiluminescence method has the advantages of high sensitivity, strong specificity, accuracy and rapidity, and short detection time, the detection result has higher accuracy and repetition performance, and the kit can be used for various luminescence detection apparatuses.

The invention discloses a method for removing ginkgo toxins in a ginkgo leaf extract. The method comprises the following steps that S1, a ginkgo leaf extracting solution is taken, and concentrating is carried out to obtain a column loading solution; the column loading liquid is fed on a macroporous adsorption resin column, water washing, impurity removal elution, primary elution and secondary elution treatment are sequentially carried out, and a primary eluant and a secondary eluant are collected; S2, the primary eluent is fed to a hydrogen type cation exchange resin column, and effluent is collected; and S3, the secondary eluent in the S1 and the effluent in the S2 are combined, concentrating is carried out, and drying is carried out to obtain the ginkgo leaf extract. According to the method for removing the ginkgo toxins in the ginkgo leaf extract, the ginkgo toxins in the ginkgo biloba extract are effectively removed, meanwhile, effective components can be recovered and retained, and the yield and quality of the ginkgo biloba extract are maintained.

The invention discloses a ractopamine magnetic particle chemiluminescent detection kit and a preparation method. The kit includes: acridinium ester markers, magnetic particles coupled with antigens or antibodies, ractopamine calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The magnetic particle chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and speed, short detection time, and higher accuracy and repeatability of detection results. The kit can be applied to various luminescent detection instruments.

The invention discloses a magnetic particle chemiluminiscence detection kit for human adrenocorticotrophic hormone and a preparation method. The kit comprises magnetic particles coupled with an adrenocorticotrophic hormone capture antibody, an adrenocorticotrophic hormone detection antibody marked by acridinium ester, an adrenocorticotrophic hormone calibrator, chemiluminiscence pre-excitation solution A, chemiluminiscence excitation solution B and cleaning solution. The kit provided by the invention employs the magnetic separation chemiluminescence technology as a detection means, and is combined with the acridinium ester labeling technology at the same time. The direct chemiluminiscence method established by the invention has the advantages of high sensitivity, strong specificity, accuracy, rapidness, short detection time and higher accuracy and repeatability of detection results, and the kit can be suitable for various luminescence detection instruments.

The invention discloses a oxytetracycline chemiluminescence detection kit and a preparation method thereof. The kit includes: acridinium ester-labeled oxytetracycline antigen or antibody, magnetic particle-coupled antibody or antigen, oxytetracycline series standard solution, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B, and cleaning solution. The kit of the invention combines the chemiluminescent technology with the immunomagnetic particle, and provides a nearly homogeneous reaction system. Compared with the prior art, the kit of the invention has the advantages of high sensitivity, strong specificity, short reaction time and the like.

The invention discloses a magnetic particle chemiluminescence detection kit of terbutaline and a preparation method thereof. The kit includes: acridinium ester markers, magnetic particles coupled with antigens or antibodies, terbutaline calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and rapidity, short detection time and higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

The invention discloses a chemiluminescence detection kit for big endothelin-1 and a preparation method thereof. The kit comprises a magnetic particle coupled big endothelin-1 (ET-1) capture antibody,an acridinium ester marked big endothelin-1 (ET-1) detection antibody, a big endothelin-1 calibration product , a chemiluminescence pre-excitation liquid A, a chemiluminescence excitation liquid B and a cleaning fluid. The kit provided by the invention combines a chemiluminescence technology and immune magnetic particles, and provides a reaction system approximate to a homogeneous phase. Comparedwith the prior art, the direct chemiluminescence method built by the invention is high in sensitivity, high in specificity, accurate, fast, short in detection time, and highly accurate and repetitivein detection result; the kit can be applicable to various luminescence detection instruments.

The invention discloses a magnetic particle chemiluminescence detection kit of clenbuterol and a preparation method thereof. The kit comprises acridinium ester markers, magnetic particles coupled with antigens or antibodies, a clenbuterol calibration product, a chemiluminescence pre-excitation solution A, a chemiluminescence excitation solution B and a washing solution B. The kit takes a magnetic separation chemiluminescence technique as a detection manner and simultaneously combines an acridinium ester marking technique; the established direct chemiluminescence method is high in sensitivity, high in specificity and short in detection time, and is accurate and rapid; the detection result has higher accuracy and repeatability and the kit can be applied to various luminescence detection instruments.

The invention relates to a detection system of a dry-type chemiluminescence method, which effectively solves the technical problems of long reaction time, easy cross infection and inaccurate detection in the prior art, and comprises a reagent card, the reagent card comprises a dry-type chromatography test strip and detection card shells, the detection card shells are buckled above and below the dry-type chromatography test strip, and the dry-type chromatography test strip and the detection card shells are connected with the reagent card. The dry-type luminescence detection system not only retains the advantages of high sensitivity, good accuracy and strong anti-interference capability of chemiluminescence detection, but also utilizes a chromatography technology for separation and avoids complex operation steps such as washing, is simple and convenient, has a fast detection speed, is suitable for large-scale industrial production, and is suitable for large-scale industrial production. In the whole operation process, the luminous starting liquid can be sucked away by the absorbent paper in the test paper card, no liquid is discharged, and the operation is convenient.

The present invention relates to 2, 2'-biquinoline synthesizing process. Inside water and polyethylene glycol (PEG) solvent system and in the presence of Pd / C catalyst, quinoline material is reacted at 140 deg.c for 4-20 hr to obtain 2, 2'-biquinoline. In the reaction system, the weight ratio between PEG and water is 0.2-2 to 1, the Pd / C catalyst has Pd supporting amount of 1-10 wt%, the PEG has molecular weight of 400-6000, the weight ratio between the Pd / C catalyst and quinoline is 0.1-1 to 1, and the weight ratio between the water / PED solvent system and quinoline is 3-10 to 1. The present invention has low material cost, simple operation, high yield and less environmental pollution, and is suitable for industrial production.

The invention relates to molecular biology and particularly discloses a gene sequence capable of preventing gradual inactivation of a promoter in subculture and applications of the gene sequence. The nucleotide sequence of the gene sequence is shown as SEQ ID No.1. The gene sequence is used for constructing a carrier and a CHO cell line, can prevent gradual inactivation of the promoter in subculture of CHO cells and can be used for monoclonal antibody production through the CHO cell line to increase the monoclonal antibody yield.

The invention discloses a complex solvent for separating benzene from sulfolane by means of extractive distillation. The complex solvent comprises 95-80 wt% of sulfolane and 5-20 wt% of co-solvent. The co-solvent is mixed xylene. The invention further relates to a method for applying the complex solvent. The complex solvent and the method have the advantages that the co-solvent comes from convenient and speedy sources, adding processes are simple and convenient, original co-solvents can be replaced, high-purity benzene products can be recycled from raw materials with high benzene contents, consumption of co-solvents can be reduced, accordingly, the production costs can be lowered, and the production and operation flexibility can be improved.

The invention relates to an extraction method of thuricin. The extraction method comprises the following process routes of: activation of thuricin bacillus; cultivation; two aqueous phase extraction to obtain liquor containing thuricin. The extraction method provided by the invention is a production process of obtaining thuricin, which is simple and convenient to operate, low in production cost, free from environmental pollution in the extraction process and free from organic solvent residues.