Salbutamol magnetic particle chemical light-emitting detection kit and preparation method

Abstract

The invention discloses a salbutamol magnetic particle chemiluminescence detection kit and a preparation method. The kit includes: acridinium ester markers, magnetic particles coupled with antigens or antibodies, albuterol calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and speed, short detection time and higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

Description

Technical field [0001] The invention belongs to the field of food safety detection, in particular to a salbutamol immunomagnetic particle chemiluminescence detection kit and a preparation method. Background technique [0002] Salbutamol (SAL), the Chinese alias is 4-(2-(tert-butylamino)-1-hydroxyethyl)-2-(hydroxymethyl)phenol, the molecular formula is C 13 H 21 NO 3 , Its structure is: [0003] [0004] It is also known as Shuchuanling, Shuchuanning, Chutening, Sorbitor, Chulaning, and oxymetrepinephrine. It is a commonly used antiasthmatic drug in clinical practice and is a selective β 2 Receptor agonists can promote the growth of skeletal muscle (lean meat), reduce fat accumulation, and improve feed conversion rate. They are often illegally used as feed additives in the production of livestock products. Salbutamol is not easy to destroy. If the animal does not pass a certain period of drug retention before slaughter, the residue will accumulate in the animal's edible tissue. Beca...

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Problems solved by technology

However, high-performance liquid chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry are expensive instruments and equipment, complicated sample pretreatment, time-consuming and labor-intensive, and difficult to popularize, and the detection cost is high

CN 103018452 A (April 2013) discloses a colloidal gold test paper card and detection method for detecting salbutamol drugs. The reaction membrane of the kit is coated with a detection line composed of salbutamol-carrier protein conjugates and coated with The quality control line composed of goat anti-mouse anti-antibody, the conjugate release pad is coated with albuterol monoclonal antibody-colloidal gold label, and then the prepared conjugate release pad, reaction membrane and sample absorbent pad, absorbent pad and backing Assemble into a test paper card, and finally use a colloidal gold test paper card for detection. The main disadvantage of this method is that the operation is cumbersome, the process is more, errors are more likely to occur, and the sensitivity is low

Although the enzyme-linked immunosorbent assay is cheap and fast, it is not sensitive enough and is only suitable for the detection and identification of trace substances. CN 103018447 A (April 2013) discloses an enzyme-linked immunosorbent assay kit and method for detecting albuterol , the kit uses a microtiter plate coated with salbutamol drug antigen, horseradish peroxidase labeled salbutamol mouse monoclonal antibody, mix the two and add to the sample, then add the substrate solution for color development, measure the absorbance value to calculate The main disadvantage of this method is the low sensitivity

CN 105315241A (February 2016) discloses a salbutamol hapten and antigen and its special chemiluminescent immunoassay kit. According to the peroxidase-labeled polyclonal antibody of rabbit origin, the sample solution to be tested is added to the microwell plate, and then the primary antibody and enzyme-labeled secondary antibody are added. Competed by the original primary antibody, then further combined with the enzyme-labeled secondary antibody, and then added to the chemiluminescence substrate solution, and calculated the content of salbutamol in the sample by the luminescence intensity value. The main disadvantage of using horseradish peroxidase is: luminol In the absence of horseradish peroxidase, H 2 o 2 Oxidation is self-luminescent, the background is relatively high, which affects the signal-to-noise ratio, the reaction kinetics is complex, there are many influencing factors, the result is not stable enough, and it is not easy to obtain a substrate with high sensitivity and long plateau period

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