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Magnetic particle chemiluminescence detection kit of clenbuterol and preparation method thereof

A chemiluminescence detection and chemiluminescence technology, applied in measurement devices, scientific instruments, instruments, etc., can solve the problems of expensive equipment, low sensitivity, and many influencing factors, and achieve good reagent stability, low background luminescence, and interference factors. less effect

Inactive Publication Date: 2017-11-17
太原瑞盛生物科技有限公司
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  • Application Information

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Problems solved by technology

However, high-performance liquid chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry are expensive instruments and equipment, complicated sample pretreatment, time-consuming and labor-intensive, and difficult to popularize, and the detection cost is high
The main disadvantages of colloidal gold immunochromatography are cumbersome operation, more processes, more prone to errors, and low sensitivity
Although the enzyme-linked immunoassay is cheap and fast, its sensitivity is not enough, and it is only suitable for the detection and identification of trace substances. CN 101290315A (October 2008) discloses a clenbuterol enzyme-linked immunoassay method and reagents Kit and its preparation method. In the kit, the clenbuterol antigen is coated on a solid phase carrier, the sample to be tested is added, and the clenbuterol bifunctional genetic engineering antibody is added. After the reaction, a substrate solution is added for color development. Determine the percent absorbance to calculate the concentration of clenbuterol in the sample. The main disadvantage of this method is low sensitivity
CN 103728450 A (April 2014) discloses a chemiluminescence detection kit for detecting clenbuterol hydrochloride. Antigen, horseradish peroxidase-labeled goat anti-rabbit clenbuterol hydrochloride polyclonal antibody, the main disadvantage of using horseradish peroxidase is that luminol will also Oxidized by H2O2 to emit light by itself, the background is relatively high, which affects the signal-to-noise ratio, the reaction kinetics is complex, there are many influencing factors, the result is not stable enough, and it is not easy to obtain a substrate with high sensitivity and long plateau period
CN 203929780 U (November 2014) discloses a magnetic particle chemiluminescence immunoassay kit for clenbuterol, which uses magnetic microspheres coupled to clenbuterol antigen, and alkaline phosphatase to label clenbuterol For Luo antibodies, the main disadvantages of using alkaline phosphatase are: the time for the substrate to reach the plateau is long, and the cost of the substrate is high, resulting in high detection costs

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  • Magnetic particle chemiluminescence detection kit of clenbuterol and preparation method thereof

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[0022] The present invention provides a clenbuterol magnetic particle chemiluminescence detection kit and a preparation method. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below.

[0023] The invention provides a clenbuterol magnetic particle chemiluminescence detection kit and a preparation method, wherein the magnetic particle chemiluminescence method detection kit for clenbuterol can adopt clenbuterol monoclonal Antibody-coupled magnetic particles, acridinium ester-labeled clenbuterol antigen, clenbuterol antigen-coupled magnetic particles, acridinium ester-labeled clenbuterol monoclonal antibody can also be used. The kit also includes clenbuterol calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution for the above-mentioned acridinium ester action.

[0024] Specifically, the inner core of the m...

Embodiment 1

[0032] Embodiment 1: The set-up and preparation method of the magnetic particle chemiluminescence kit 1 for detecting clenbuterol includes the following steps:

[0033] 1. The composition of kit 1:

[0034] Construct a magnetic particle chemiluminescence kit for the detection of clenbuterol so that it contains the following components:

[0035] Clenbuterol monoclonal antibody coupled to carboxyl magnetic particles;

[0036] Acridinium ester-labeled clenbuterol antigen;

[0037] Clenbuterol series standard solution, the concentrations are: 0 μg / L, 0.01 μg / L, 0.06 μg / L, 0.36 μg / L, 2.16 μg / L, 21.6 μg / L, the buffer contains 1- 3% BSA and 0.1-0.3% PC300 in Tris-HCl solution;

[0038] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0039] The cleaning solution is specifically Tris-HCl buffer solution (pH 7.2) with a concentration of 25 mmol / L, which contains NaCl and 0.05% Tween-20 with a concentration of 0.15 mol / L.

[0040] 2. Prepar...

Embodiment 2

[0058] Embodiment 2: The establishment and preparation method of a magnetic particle chemiluminescence kit 2 for detecting clenbuterol, comprising the following steps:

[0059] 1. The formation of kit 2:

[0060] Construct a magnetic particle chemiluminescence kit for the detection of clenbuterol so that it contains the following components:

[0061] Clenbuterol antigen coupled to carboxyl magnetic particles;

[0062] Acridinium ester-labeled clenbuterol monoclonal antibody;

[0063] Clenbuterol series standard solution, the concentrations are: 0 μg / L, 0.01 μg / L, 0.06 μg / L, 0.36 μg / L, 2.16 μg / L, 21.6 μg / L, the buffer contains 1- 3% BSA and 0.1-0.3% PC300 in Tris-HCl solution;

[0064] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0065] The cleaning solution is specifically Tris-HCl buffer solution (pH 7.2) with a concentration of 25 mmol / L, which contains NaCl and 0.05% Tween-20 with a concentration of 0.15 mol / L.

[0066] 2. ...

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Abstract

The invention discloses a magnetic particle chemiluminescence detection kit of clenbuterol and a preparation method thereof. The kit comprises acridinium ester markers, magnetic particles coupled with antigens or antibodies, a clenbuterol calibration product, a chemiluminescence pre-excitation solution A, a chemiluminescence excitation solution B and a washing solution B. The kit takes a magnetic separation chemiluminescence technique as a detection manner and simultaneously combines an acridinium ester marking technique; the established direct chemiluminescence method is high in sensitivity, high in specificity and short in detection time, and is accurate and rapid; the detection result has higher accuracy and repeatability and the kit can be applied to various luminescence detection instruments.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to a clenbuterol immune magnetic particle chemiluminescent detection kit and a preparation method. Background technique [0002] Clenbuterol (ractopamine, RAC), the scientific name is clenbuterol hydrochloride, also known as clenbuterol, ammonium, ambiturate, ammonium dichloride, and its molecular formula is C 12 h 18 C l2 N 2 O, whose structure is: [0003] [0004] In China, what is commonly referred to as "Clenbuterol" refers to Clenbuterol. Clenbuterol is a potent selective beta 2 Receptor agonist, well absorbed in domestic animals and humans, and compared with other β-agonists, it has high bioavailability. In the early 1980s, a company in the United States began to add it to feed to increase lean meat rate, but if it is used as a feed additive, the dosage is more than 10 times that of human medicine, in order to achieve the effect of increasing the lean meat rate. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/577
CPCG01N33/54326G01N33/577
Inventor 常燕胡雪婷曹晶刘丽青杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
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