Magnetic particle chemiluminescence detection kit for pregnancy-associated plasma protein A, and preparation method of detection kit

Abstract

The invention discloses a magnetic particle chemiluminescent detection kit for pregnancy-related plasma protein A and a preparation method thereof. The kit includes: magnetic particles coupled with pregnancy-associated plasma protein A capture antibody, acridinium ester-labeled pregnancy-associated plasma protein A detection antibody, pregnancy-associated plasma protein A calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and rapidity, short detection time and higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

Description

technical field [0001] The invention belongs to the technical field of immunodetection and analysis, in particular to an immunomagnetic particle chemiluminescence detection kit and a preparation method of pregnancy-related plasma protein A. Background technique [0002] Pregnancy-Associated Plasma Protein A (Pregnancy-Associated Plasma Protein A, PAPP-A) is a macromolecular glycoprotein compound isolated from serum of pregnant women in 1974, produced by placental syncytiotrophoblast and decidual cells. Its structure is a heteropolymer composed of two PAPP-A molecules and two eosinphilmajor basic protein precursors (proform of eosinphilmajor basic protein, proMBP), with a relative molecular mass of 474,000. The content of PAPP-A in the peripheral blood of pregnant women with miscarriage, threatened miscarriage, ectopic pregnancy and malformed fetuses is lower than that of normal pregnant women at the same gestational age. Low levels of PAPP-A in the early pregnancy stage can ...

AI Technical Summary

Problems solved by technology

Although this method is cheap and fast, the enzyme-labeled method itself is restricted by solid-phase reactions, and the stability of the enzyme-labeled color is also easily affected by changes in temperature and pH. Most of the substrates used are toxic substances or carcinogens. And its sensitivity is not enough, it is only suitable for the detection and identification of trace substances, and it can no longer meet the requirements of rapid quantitative detection in large hospitals

CN1210267A (March 1999) discloses a kit for the quantitative detection of pregnancy-related plasma protein A by enzyme-linked assay. However, during the whole pregnancy process, the level of serum proMBP greatly exceeds that of PAPP-A, so that the measurement results of PAPP-A cannot truly reflect its level in the blood, and the detection is accurate decreased sex

The time-resolved fluorescence immunoassay has high accuracy and specificity, but is slow, and the equipment is expensive, and the cost is relatively high, so it is not easy to promote and use in primary medical institutions.