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Oxytetracycline chemiluminescence detection kit and preparation method thereof

A chemiluminescence detection and chemiluminescence technology, which is applied in the direction of chemiluminescence/bioluminescence, analysis by causing chemical reactions of materials, and measurement devices, can solve the problems of large error in results, low sensitivity, and high detection cost, and achieve photon production. The effect of no reduction in efficiency, good reagent stability, and low background luminescence

Inactive Publication Date: 2017-11-24
太原瑞盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbiological method has poor specificity, cumbersome operation, long detection cycle and large error in results
CN 104374907A (February 2015) discloses a kit for detecting oxytetracycline by enzyme-linked immunosorbent assay. The disadvantage of using enzyme-linked immunosorbent assay for detection is that horseradish peroxidase or alkaline phosphate markers are easily inactivated, which is obvious. The chromatic substrate is easy to decompose when exposed to light, has low sensitivity, and has a certain cross-reaction to compounds with similar structures, resulting in inaccurate test results, and is only suitable for the detection and identification of trace substances
CN 103823005 A (May 2014) discloses a method for detecting oxytetracycline by high-performance liquid chromatography. The disadvantage of using high-performance liquid chromatography is that the sample pretreatment is complicated, laborious and time-consuming, and the detection cost is high
CN 1818658 A (August 2006) discloses a kit for colloidal gold detection of oxytetracycline. The disadvantage of using the colloidal gold method for detection is that the final detection result is characterized by a color visible to the naked eye, and the error is relatively large, and the operation It is cumbersome, has many processes, is more prone to errors, and has low sensitivity

Method used

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preparation example Construction

[0022] The invention provides a oxytetracycline chemiluminescent detection kit and a preparation method, wherein the oxytetracycline detection kit provided by the present invention can use oxytetracycline antibody-coupled magnetic particles, acridinium ester To mark oxytetracycline, oxytetracycline antigen-coupled magnetic particles can also be used, and acridinium ester-labeled oxytetracycline antibody can be used. The kit also includes oxytetracycline calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution.

[0023] Specifically, in the process of preparing magnetic particle coupling in the present invention, the buffer solution for coupling antigen is a buffer solution with a pH value of 5.0 and a concentration of 0.1 mol / L MES; the buffer solution for coupling antibody is a buffer solution with a pH value of 5.0 6.0. Buffer solution with a concentration of 0.1 mol / L MES.

[0024] Specifically, in the process o...

Embodiment 1

[0030] Example 1: The composition and preparation method of a oxytetracycline chemiluminescence detection kit 1

[0031] 1. Assembly of Kit 1

[0032] Oxytetracycline antigen labeled with acridinium ester;

[0033] Oxytetracycline antibody coupled to magnetic particles;

[0034] Oxytetracycline series calibrator solutions, concentrations are: 0 μg / L, 0.4 μg / L, 2 μg / L, 10 μg / L, 50 μg / L, 250 μg / L;

[0035] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0036] Cleaning solution, including the following components: Tris-HCl with a pH of 7.2 and a concentration of 25 mmol / L, which contains NaCl and 0.05% Tween-20 with a concentration of 0.15 mmol / L.

[0037] 2. Preparation of Magnetic Microparticle Suspensions Conjugated to Oxytetracycline Antibody

[0038](1) Take 1 mg of carboxyl magnetic particles in a 0.5 mL centrifuge tube, add a certain amount of 0.1 mol / L MES buffer, vortex and mix, place on a magnetic stand, and let it stand f...

Embodiment 2

[0055] Embodiment 2: The composition and preparation method of a oxytetracycline chemiluminescent detection kit 2

[0056] 1. Assembly of Kit 2

[0057] Oxytetracycline antibody labeled with acridinium ester;

[0058] Oxytetracycline antigen coupled to magnetic particles;

[0059] Oxytetracycline series calibrator solutions, concentrations are: 0 μg / L, 0.4 μg / L, 2 μg / L, 10 μg / L, 50 μg / L, 250 μg / L;

[0060] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0061] Cleaning solution, including the following components: Tris-HCl with a pH of 7.2 and a concentration of 25 mmol / L, which contains NaCl and 0.05% Tween-20 with a concentration of 0.15 mmol / L.

[0062] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Oxytetracycline Antigen

[0063] (1) Take 1 mg of carboxyl magnetic particles in a 0.5 mL centrifuge tube, add a certain amount of 0.1 mol / L MES buffer, vortex and mix, place on a magnetic stand, and let it stan...

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Abstract

The invention discloses a oxytetracycline chemiluminescence detection kit and a preparation method thereof. The kit includes: acridinium ester-labeled oxytetracycline antigen or antibody, magnetic particle-coupled antibody or antigen, oxytetracycline series standard solution, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B, and cleaning solution. The kit of the invention combines the chemiluminescent technology with the immunomagnetic particle, and provides a nearly homogeneous reaction system. Compared with the prior art, the kit of the invention has the advantages of high sensitivity, strong specificity, short reaction time and the like.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to an immunochemiluminescent detection kit for oxytetracycline and a preparation method thereof. Background technique [0002] Oxytetracycline, alias: oxytetracycline dihydrate or oxytetracycline. It has a broad-spectrum anti-pathogenic microorganism effect, is a rapid bacteriostatic agent, and has a bactericidal effect on some bacteria at high concentrations. It also has a strong inhibitory effect on Rickettsia, Mycoplasma, Chlamydia, Actinomycetes and Amoeba. However, oxytetracycline can enter the fetus through the blood-placental barrier and deposit in the calcium areas of teeth and bones, causing fetal tooth discoloration, poor enamel regeneration, and inhibition of fetal bone growth. In addition, taking oxytetracycline can also cause imbalance of normal flora in the body, cause vitamin deficiency, fungal reproduction, dry mouth, sore throat, angular cheilitis, glossitis, ...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/543
CPCG01N21/76G01N33/54326
Inventor 曹晶刘丽青胡雪婷常燕杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
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