Ractopamine magnetic particle chemiluminiscence detection kit and preparation method thereof

Abstract

The invention discloses a ractopamine magnetic particle chemiluminescent detection kit and a preparation method. The kit includes: acridinium ester markers, magnetic particles coupled with antigens or antibodies, ractopamine calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The magnetic particle chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and speed, short detection time, and higher accuracy and repeatability of detection results. The kit can be applied to various luminescent detection instruments.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to a ractopamine immunomagnetic particle chemiluminescence detection kit and a preparation method. Background technique [0002] Ractopamine (RAC), Chinese alias 4-[3-[2-hydroxy-2-(4-hydroxyphenyl)-ethyl]aminobutyl]phenol, molecular formula is C 18 h 23 NO 3 , whose structure is: [0003] [0004] It is a synthetic β-adrenoreceptor agonist of Clonbacamine, which is a β-stimulant compound and belongs to the second generation of clenbuterol. β-agonists, also known as β-agonists, are a class of phenethylamines with chemical structures and physiological functions similar to epinephrine and norepinephrine. Studies in the 1980s showed that β-stimulants affected the flow and redistribution of nutrients in animals, enhanced fat catabolism and protein synthesis in the body, and significantly increased lean meat percentage. Residues of β-stimulants will accumulate in the edible ti...

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Problems solved by technology

Although enzyme-linked immunoassay is cheap and fast, it is not sensitive enough and is only suitable for the detection and identification of trace substances. CN 101315379 (December 2008) discloses a kit for detecting ractopamine and its application. The kit uses ractopamine-coated ELISA plates, and horseradish peroxidase-labeled ractopamine antibodies. The disadvantage of this method is low sensitivity

CN 105759042 A (July 2016) discloses a chemiluminescent enzyme-linked immunoassay kit for detecting ractopamine. The kit uses ractopamine monoclonal antibody-coated polystyrene chemiluminescence plate, horseradish peroxidase The artificial antigen made by coupling labeled ractopamine and bovine serum albumin, the main disadvantage of using horseradish peroxidase is: luminol will also be absorbed by H in the absence of horseradish peroxidase 2 o 2 Oxidation is self-luminescent, the background is relatively high, which affects the signal-to-noise ratio, the reaction kinetics is complex, there are many influencing factors, the result is not stable enough, and it is not easy to obtain a substrate with high sensitivity and long plateau period

CN 106568943 A (April 2017) discloses a detection kit for ractopamine in food, the kit uses amino magnetic beads to coat ractopamine, alkaline phosphatase labels ractopamine-binding protein, and uses alkaline phosphatase to The main disadvantages are: the time for the substrate to reach the plateau is long, and the cost of the substrate is high, resulting in high detection cost

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