Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Leptin chemiluminescent detection kit and preparation method thereof

A chemiluminescence detection and chemiluminescence technology, which is applied in the direction of biological testing, measuring devices, scientific instruments, etc., can solve the problems of complex reaction kinetics, unstable results, and many influencing factors, and achieve low cost, good reagent stability, Low background glow effect

Inactive Publication Date: 2018-05-18
太原瑞盛生物科技有限公司
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of using horseradish peroxidase is that luminol will also be destroyed by H in the absence of horseradish peroxidase. 2 o 2 Oxidation is self-luminescent, the background is relatively high, which affects the signal-to-noise ratio, the reaction kinetics is complex, there are many influencing factors, the result is not stable enough, and it is not easy to obtain a substrate with high sensitivity and long plateau period

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] The present invention provides a leptin chemiluminescent detection kit and a preparation method. In order to make the purpose, technical solution and effect of the present invention more definite and clear, the present invention will be further described in detail below.

[0024] The present invention provides a leptin chemiluminescence detection kit and a preparation method, wherein the leptin detection kit provided by the present invention uses magnetic particles coupled to a leptin capture antibody, and acridinium ester-labeled leptin Detection antibody. The kit also includes leptin calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution for the above-mentioned acridinium ester action.

[0025] Specifically, the magnetic particle-coupled suspension of the present invention is composed of leptin-capturing antibody-coupled magnetic particles and reagent buffer, wherein the concentration of leptin-capturing ...

Embodiment 1

[0035] Embodiment 1: The formation and preparation method of a kind of chemiluminescent kit for detecting leptin, comprising the following steps:

[0036] 1. The composition of the kit:

[0037] Construct a chemiluminescent kit for the detection of leptin so that it contains the following components:

[0038] Magnetic particle-coupled leptin monoclonal antibody;

[0039] Another leptin monoclonal antibody labeled with acridinium ester;

[0040] Leptin series standard solution;

[0041] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0042] Cleaning fluid.

[0043] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Leptin Monoclonal Antibody

[0044] (1) Take 1 mg of carboxyl magnetic particles in a 0.5 mL centrifuge tube, add 200 μL of MES buffer solution with a concentration of 0.1 mol / L, vortex and mix, place on a magnetic stand, and let it stand for 5 minutes to make the magnetic particles and liquid Separate...

Embodiment 2

[0064] Embodiment 2: The steps of using the leptin chemiluminescence detection kit to detect are:

[0065] (1) Add 50 μL of the sample to be tested into the cuvette, then add 150 μL of magnetic particle coupling suspension, shake and mix, and incubate at 37°C for 8 min.

[0066] (2) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0067] (3) Add 150 μL of acridinium ester marker into the cuvette, shake to mix, and incubate at 37°C for 7 min.

[0068] (4) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0069] (5) Add 100 μL of chemiluminescence pre-excitation solution A, add 100 μL of chemiluminescence excitation solution B after 1 s, and measure its relative luminous intensity. The content of leptin in the sample is proportional to its relative luminous intensity.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Analytical sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a leptin chemiluminescent detection kit and a preparation method thereof. The kit comprises: magnetic particles coupled to a leptin capture antibody, a leptin detection antibody labelled by acridinium ester, a leptin calibration product, a chemiluminescent pre-triggered solution A, a chemiluminescent triggered solution B and a cleaning fluid. According to the kid, magneticseparation chemiluminescent technology is taken as a detection means, and is combined with acridinium ester labelling technology. The established direct chemiluminescent method is high in sensitivity,good in specificity, and short in detection time, and is accurate and fast. The detection result is high in accuracy and repeatability, and the kit is suitable for various luminescent detection apparatuses.

Description

technical field [0001] The invention belongs to the technical field of immunological detection and analysis, in particular to a leptin chemiluminescent detection kit and a preparation method thereof. Background technique [0002] Leptin is a protein with 167 amino acid residues encoded by the ob gene. Leptin secreted into plasma is a protein composed of 146 amino acid residues after removing 21 amino acid residues from the nerve terminal signal peptide, with a molecular weight of about 16kD and strong hydrophilicity. Most of it (about 80%) in plasma is combined with plasma protein, and a small part exists in free form to exert biological effects. Plasma leptin levels were significantly positively correlated with the amount of ob mRNA in adipose tissue, body mass index, sex, and body fat percentage. [0003] Current research shows that leptin has a wide range of biological effects, the most important of which is that it acts on the hypothalamus body weight regulation center...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/6803G01N33/577G01N2333/575
Inventor 常燕胡雪婷曹晶刘丽青杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com