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Magnetic particle chemiluminescent detection kit for terbutaline and preparation method

A chemiluminescence detection and terbutaline technology, which is applied in the direction of measuring devices, scientific instruments, instruments, etc., can solve the problems of complex reaction kinetics, unstable results, and many influencing factors, and achieve low cost and good reagent stability , the effect of low background glow

Inactive Publication Date: 2017-11-17
太原瑞盛生物科技有限公司
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Problems solved by technology

CN 101393210 A (March 2009) discloses a chemiluminescent enzyme-linked immunoassay kit for terbutaline. The kit uses an artificial antigen prepared by coupling terbutaline and ovalbumin coated with a microtiter plate , horseradish peroxidase-labeled terbutaline polyclonal antibody, although it has achieved the effect of saving time, labor and cost, but the use of horseradish peroxidase has the following disadvantages: In the presence of the enzyme, it will also be blocked by the H 2 o 2 Oxidation is self-luminescent, the background is relatively high, which affects the signal-to-noise ratio, the reaction kinetics is complex, there are many influencing factors, the result is not stable enough, and it is not easy to obtain a substrate with high sensitivity and long plateau period

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  • Magnetic particle chemiluminescent detection kit for terbutaline and preparation method

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preparation example Construction

[0022] The present invention provides a magnetic particle chemiluminescent detection kit for terbutaline and a preparation method thereof. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below.

[0023] The present invention provides a magnetic particle chemiluminescent detection kit for terbutaline and a preparation method thereof, wherein the magnetic particle chemiluminescent detection kit for terbutaline can be terbutaline monoclonal Antibody-coupled magnetic particles, acridinium ester-labeled terbutaline antigen, or terbutaline antigen-coupled magnetic particles, acridinium-ester-labeled terbutaline monoclonal antibody. The kit also includes terbutaline calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution for the above-mentioned acridinium ester action.

[0024] Specifically, the inner core of ...

Embodiment 1

[0032] Embodiment 1: The set-up and preparation method of a magnetic particle chemiluminescence kit 1 for detecting terbutaline, comprising the following steps:

[0033] 1. The composition of kit 1:

[0034] A magnetic particle chemiluminescence kit for detecting terbutaline was constructed so that it contained the following components:

[0035] Terbutaline monoclonal antibody coupled to carboxyl magnetic particles;

[0036] acridinium ester-labeled terbutaline antigen;

[0037] Terbutaline series standard solution, the concentrations are: 0 μg / L, 0.02 μg / L, 0.1 μg / L, 0.5 μg / L, 2.5 μg / L, 12.5 μg / L, the buffer contains 1- 3% BSA and 0.1-0.3% PC300 in Tris-HCl solution;

[0038] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0039] The cleaning solution is specifically Tris-HCl buffer solution (pH 7.2) with a concentration of 25 mmol / L, which contains NaCl and 0.05% Tween-20 with a concentration of 0.15 mol / L.

[0040] 2. Preparatio...

Embodiment 2

[0058] Embodiment 2: The set-up and preparation method of a magnetic particle chemiluminescence kit 2 for detecting terbutaline, comprising the following steps:

[0059] 1. The formation of kit 2:

[0060] A magnetic particle chemiluminescence kit for detecting terbutaline was constructed so that it contained the following components:

[0061] Terbutaline antigen coupled with carboxyl magnetic particles;

[0062] Acridine ester-labeled terbutaline monoclonal antibody;

[0063] Terbutaline series standard solution, the concentrations are: 0 μg / L, 0.02 μg / L, 0.1 μg / L, 0.5 μg / L, 2.5 μg / L, 12.5 μg / L, the buffer contains 1- 3% BSA and 0.1-0.3% PC300 in Tris-HCl solution;

[0064] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0065] The cleaning solution is specifically Tris-HCl buffer solution (pH 7.2) with a concentration of 25 mmol / L, which contains NaCl and 0.05% Tween-20 with a concentration of 0.15 mol / L.

[0066] 2. Preparatio...

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Abstract

The invention discloses a magnetic particle chemiluminescence detection kit of terbutaline and a preparation method thereof. The kit includes: acridinium ester markers, magnetic particles coupled with antigens or antibodies, terbutaline calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and rapidity, short detection time and higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to a terbutaline immune magnetic particle chemiluminescence detection kit and a preparation method. Background technique [0002] Terbutaline, Chinese alias 5-(1-hydroxy-2-tert-butylaminoethyl) benzene-1,3-diol, molecular formula C 12 h 19 NO 3 , whose structure is: [0003] [0004] Also known as terbutaline and terbutaline, it is often used clinically to treat bronchial asthma, asthmatic bronchitis, emphysema, etc. Its bronchodilator effect is weaker than that of salbutamol, and it is also selective β 2 Receptor agonists can promote the growth of skeletal muscle (lean meat) in animals, reduce fat accumulation, and increase carcass lean meat percentage. They are often illegally used as feed additives in the production of livestock products. Terbutaline is not easy to destroy. If the animal does not pass a certain period of drug residence before slaughter, the residue wil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/577
CPCG01N33/54326G01N33/577
Inventor 常燕胡雪婷曹晶刘丽青杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
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