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Method for removing ginkgo toxins in ginkgo leaf extract

A technology of ginkgo biloba and extract, which is applied in the field of compound purification, can solve the problems of the content of active ingredients and the reduction of yield, and achieve the effects of no reduction in yield, reduction of production cost, and convenient operation

Active Publication Date: 2021-10-12
JIANGSU HUIKANG BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Ginkgo toxin can be removed by adding dilute ethanol elution steps, but while dilute ethanol elution removes ginkgo toxin, part of the active ingredients are also removed, although the ginkgo toxin content in the prepared ginkgo leaf extract< 5ppm, but the active ingredient content and yield decrease

Method used

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  • Method for removing ginkgo toxins in ginkgo leaf extract

Examples

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Effect test

Embodiment 1

[0039] A method for removing ginkgotoxin in ginkgo leaf extract, comprising the steps of:

[0040] S1, get 1000g of ginkgo leaves, pulverize, heat and reflux with dilute ethanol to extract 2 times, combine the extracts to obtain ginkgo leaf extracts; concentrate the ginkgo leaf extracts to obtain 3000ml of upper column liquid;

[0041] Add the upper column solution to the pretreated macroporous adsorption resin column with a column volume of 1000ml, wash with 500ml water successively, remove impurities and elute with 2000ml of ethanol aqueous solution with a volume fraction of 5%, and elute with 3000ml of ethanol aqueous solution with a volume fraction of 17%. First elution, 3000ml volume fraction of 50% ethanol aqueous solution for second elution, collect the first eluate and the second eluate;

[0042] S2, the pretreated column volume on the primary eluent is 100ml of 001 × 7 hydrogen-type cation exchange resin columns, and the effluent is collected; then the hydrogen-type c...

Embodiment 2

[0045] A method for removing ginkgotoxin in ginkgo leaf extract, comprising the steps of:

[0046] S1, get 1000g of ginkgo leaves, pulverize, heat and reflux with dilute ethanol to extract 2 times, combine the extracts to obtain ginkgo leaf extracts; concentrate the ginkgo leaf extracts to obtain 3000ml of upper column liquid;

[0047] Add the upper column solution to the pretreated macroporous adsorption resin column with a column volume of 1000ml, wash with 1000ml water successively, 1500ml volume fraction of 10% ethanol aqueous solution for impurity removal, 2000ml volume fraction of 25% ethanol aqueous solution First elution, 2500ml volume fraction of 65% ethanol aqueous solution for second elution, collect the first eluate and the second eluate;

[0048] S2, the 732 hydrogen-type cation exchange resin column of 100ml that the column volume after the pretreatment on the primary eluent is used to collect the effluent; then the hydrogen-type cation-exchange resin column is s...

Embodiment 3

[0051] A method for removing ginkgotoxin in ginkgo leaf extract, comprising the steps of:

[0052] S1, get 1000g of ginkgo leaves, pulverize, heat and reflux with dilute ethanol to extract 2 times, combine the extracts to obtain ginkgo leaf extracts; concentrate the ginkgo leaf extracts to obtain 3000ml of upper column liquid;

[0053] Add the upper column solution to the pretreated macroporous adsorption resin column with a column volume of 1000ml, wash with 2000ml water successively, remove impurities and elute with 1000ml of ethanol aqueous solution with a volume fraction of 15%, and remove impurities with 1000ml of ethanol aqueous solution with a volume fraction of 30%. First elution, 2000ml volume fraction of 80% ethanol aqueous solution for second elution, collect the first eluate and the second eluate;

[0054] S2, the pretreated column volume on the primary eluent is a 732 hydrogen-type cation exchange resin column of 100ml, and the effluent is collected; then the hydr...

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Abstract

The invention discloses a method for removing ginkgo toxins in a ginkgo leaf extract. The method comprises the following steps that S1, a ginkgo leaf extracting solution is taken, and concentrating is carried out to obtain a column loading solution; the column loading liquid is fed on a macroporous adsorption resin column, water washing, impurity removal elution, primary elution and secondary elution treatment are sequentially carried out, and a primary eluant and a secondary eluant are collected; S2, the primary eluent is fed to a hydrogen type cation exchange resin column, and effluent is collected; and S3, the secondary eluent in the S1 and the effluent in the S2 are combined, concentrating is carried out, and drying is carried out to obtain the ginkgo leaf extract. According to the method for removing the ginkgo toxins in the ginkgo leaf extract, the ginkgo toxins in the ginkgo biloba extract are effectively removed, meanwhile, effective components can be recovered and retained, and the yield and quality of the ginkgo biloba extract are maintained.

Description

technical field [0001] The invention relates to the technical field of compound purification, in particular to a method for removing ginkgotoxin from ginkgo biloba extract. Background technique [0002] Ginkgo biloba is one of the oldest species in the world. It grows slowly and has an extremely long life span. It is known as a living fossil. Its strong vitality attracts people to explore more about its application prospects in health, food, and nutritional supplements. The part of ginkgo with great medicinal value is the leaf, which has unique medicinal value and application value. For decades, people have used Ginkgo biloba extract as medicine because it contains flavonoids, terpene lactones and other related active ingredients used to treat dementia, cerebral thrombosis and other diseases. However, with the continuous deepening of research, people found that there are active ingredients such as organic acids, flavonol glycosides, and terpene lactones in Ginkgo biloba ex...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61K127/00
CPCA61K36/16A61K2236/55A61K2236/51Y02A50/30
Inventor 陈金平张超夏天缘
Owner JIANGSU HUIKANG BIOLOGICAL
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