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Magnetic particle chemiluminiscence detection kit for vascular endothelial growth factor receptor 1 and preparation method

A technology for detection of growth factor receptors and chemiluminescence, which can be used in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, and measurement devices, etc. It can solve the problems of insufficient sensitivity and achieve low background luminescence and signal-to-noise ratio High and low cost effect

Inactive Publication Date: 2018-03-16
太原瑞盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method is cheap and fast, its sensitivity is not enough, so it is only suitable for the detection and identification of trace substances.

Method used

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Examples

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Effect test

preparation example Construction

[0022] The present invention provides a magnetic particle chemiluminescent detection kit for vascular epidermal growth factor receptor 1 and a preparation method. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below.

[0023] The present invention provides a magnetic particle chemiluminescence detection kit for intravascular epidermal growth factor receptor 1 and a preparation method thereof, wherein the magnetic particle chemiluminescence method provided by the present invention detects intravascular epidermal growth factor receptor 1 kit, The magnetic particle-coupled intravascular epidermal growth factor receptor 1 capture antibody was used, and the acridinium ester-labeled intravascular epidermal growth factor receptor 1 detection antibody was used. The kit also includes intravascular epidermal growth factor receptor 1 calibrator, chemiluminescence pre-excitati...

Embodiment 1

[0034] Embodiment 1: The establishment and preparation method of a magnetic particle chemiluminescence kit for detecting intravascular epidermal growth factor receptor 1 includes the following steps:

[0035] 1. The composition of the kit:

[0036] A magnetic particle chemiluminescence kit for detecting intravascular epidermal growth factor receptor 1 was constructed so that it contained the following components:

[0037] Intravascular epidermal growth factor receptor 1 monoclonal antibody coupled to magnetic particles;

[0038] Another intravascular epidermal growth factor receptor 1 monoclonal antibody labeled with acridinium ester;

[0039] Intravascular epidermal growth factor receptor 1 series standard solution;

[0040] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0041] Cleaning fluid.

[0042] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Intravascular Epidermal Growth Factor Receptor 1 Monoclonal ...

Embodiment 2

[0063] Embodiment 2: The steps for detecting using the magnetic particle chemiluminescent detection kit for intravascular epidermal growth factor receptor 1 are as follows:

[0064] (1) Add 50 μL of the sample to be tested into the cuvette, then add 150 μL of magnetic particle coupling suspension, shake and mix, and incubate at 37°C for 8 min.

[0065] (2) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0066] (3) Add 150 μL of acridinium ester marker into the cuvette, shake to mix, and incubate at 37°C for 7 min.

[0067] (4) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0068] (5) Add 100 μL of chemiluminescence pre-excitation solution A, add 100 μL of chemiluminescence excitation solution B after 1 s, and measure its relative luminous intensity. The content of vascular epidermal growth factor receptor 1 in the sample is proportional to its relative lumi...

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Abstract

The invention discloses a magnetic particle chemiluminiscence detection kit for a vascular endothelial growth factor receptor 1 and a preparation method. The kit comprises vascular endothelial growthfactor receptor 1 capture antibody coupled magnetic particles, an acridinium ester marked vascular endothelial growth factor receptor 1 detecting antibody, a vascular endothelial growth factor receptor 1 calibrator, chemiluminiscence pre-excitation liquid A, chemiluminiscence pre-excitation liquid B and cleaning liquid. The kit adopts a magnetic separation chemiluminiscence technology as a detection means combined with an acridinium ester marking technology. A direct chemiluminiscence method is high in sensitivity and specificity, accurate, quick and short in detection time, detection resultsare high in accuracy and repeatability, and the kit is applicable to various luminescence detection instruments.

Description

technical field [0001] The invention belongs to the technical field of immunodetection and analysis, in particular to an immunomagnetic microparticle chemiluminescent detection kit for intravascular epidermal growth factor receptor 1 and a preparation method. Background technique [0002] Soluble vascular endothelial growth factor receptor-1 (sVEGFR-1), also known as soluble fms-like tyrosine kinase-1 (sFlt-1), sVEGFR-1 lacks the seventh VEGFR-1 Immunoglobulin-like domain, lacking intracellular tyrosine kinase domain, so it has no cell signal transduction function, but it still has the ability to bind with high affinity to vascular endothelial growth factor (VEGF) and placental growth factor (PIGF) . sVEGFR-1 mainly plays an anti-VEGF biological function in vivo, which is mainly manifested in: (1) anti-angiogenesis, inhibiting the VEGF-VEGFR-2 signal that promotes angiogenesis; (2) through VEGR-1 or VEGFR-2 Interfering with VEGF-mediated vascular permeability; (3) producin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N21/76
CPCG01N33/577G01N21/76
Inventor 许殊荣程月萍李瑶杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
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