Magnetic particle chemiluminescence detection kit for calcitonin and preparation method thereof

A chemiluminescence detection and chemiluminescence technology, which can be used in chemiluminescence/bioluminescence, biological testing, and analysis by chemical reaction of materials. It can solve the problems of high substrate cost, high detection cost, and many influencing factors. Achieve the effects of good reagent stability, low background luminescence, and few interference factors

Inactive Publication Date: 2018-01-05
太原瑞盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of using horseradish peroxidase is that luminol will also be destroyed by H in the absence of horseradish peroxidase. 2 o 2 Oxidation is self-luminescent, the background is relatively high, which affects the signal-to-noise ratio, the reaction kinetics is complex, there are many influencing factors, the result is not stable enough, it is not easy to obtain a substrate with high sensitivity and long plateau period; the main disadvantage of using alkaline phosphatase For: the time for the substrate to reach the plateau is long, and the cost of the substrate is high, resulting in high detection cost

Method used

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preparation example Construction

[0022] The present invention provides a calcitonin magnetic particle chemiluminescent detection kit and a preparation method. In order to make the purpose, technical scheme and effect of the present invention more definite and clear, the present invention will be further described in detail below.

[0023] The present invention provides a magnetic particle chemiluminescence detection kit for calcitonin and a preparation method thereof, wherein the magnetic particle chemiluminescence method detection kit for calcitonin provided by the present invention uses magnetic particle-coupled calcitonin capture antibody , an acridinium ester-labeled calcitonin detection antibody. The kit also includes calcitonin calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution for the above-mentioned acridinium ester action.

[0024] Specifically, the magnetic particle-coupled suspension of the present invention consists of magnetic pa...

Embodiment 1

[0034] Embodiment 1: The formation and preparation method of a magnetic particle chemiluminescence kit for detecting calcitonin includes the following steps:

[0035] 1. The composition of the kit:

[0036] A magnetic particle chemiluminescence kit for detecting calcitonin is assembled so that it contains the following components:

[0037] Calcitonin monoclonal antibody coupled to magnetic particles;

[0038] Another calcitonin monoclonal antibody labeled with acridinium ester;

[0039] Calcitonin series standard solution;

[0040] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0041] Cleaning fluid.

[0042] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Calcitonin Monoclonal Antibody

[0043] (1) Take 1 mg of carboxyl magnetic particles in a 0.5 mL centrifuge tube, add 200 μL of MES buffer solution with a concentration of 0.1 mol / L, vortex and mix, place on a magnetic stand, and let it stand for 5 minutes ...

Embodiment 2

[0063] Embodiment 2: The steps for detecting using the magnetic particle chemiluminescence detection kit of calcitonin are:

[0064] (1) Add 50 μL of the sample to be tested into the cuvette, then add 150 μL of magnetic particle coupling suspension, shake and mix, and incubate at 37°C for 8 min.

[0065] (2) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0066] (3) Add 150 μL of acridinium ester marker into the cuvette, shake to mix, and incubate at 37°C for 7 min.

[0067] (4) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0068] (5) Add 100 μL of chemiluminescence pre-excitation solution A, add 100 μL of chemiluminescence excitation solution B after 1 s, and measure its relative luminous intensity. The content of calcitonin in the sample is proportional to its relative luminous intensity.

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Abstract

The invention discloses a calcitonin magnetic particle chemiluminescent detection kit and a preparation method. The kit includes: magnetic particles coupled with calcitonin capture antibody, acridinium ester-labeled calcitonin detection antibody, calcitonin calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and rapidity, short detection time and higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

Description

technical field [0001] The invention belongs to the technical field of immune detection and analysis, in particular to a calcitonin immune magnetic particle chemiluminescent detection kit and a preparation method. Background technique [0002] Calcitonin (CT) is a single peptide chain hormone, which is synthesized and secreted by thyroid parafollicular cells and mainly metabolized by the kidneys. It can reduce the concentration of calcium and phosphorus in plasma, inhibit the absorption of calcium and phosphorus, and is an antagonist of parathyroid hormone. The main target organs of its biological activity are bone and kidney. Usually the increase and decrease of calcium level marks the secretion of calcitonin, and the level of calcium in circulation increases rapidly, and the level of calcitonin also rises rapidly. Calcitonin has a half-life of 25-30 hours in the human body, good stability, and its content in normal human serum is extremely low. The clinical significance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N21/76
Inventor 常燕胡雪婷曹晶刘丽青杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
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