Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Magnetic particle chemiluminescence detection kit for human soluble ST2 and preparation method

A chemiluminescence detection, chemiluminescence technology, applied in chemiluminescence/bioluminescence, biological testing, analysis by chemical reaction of materials, etc., can solve the problems of high detection cost, long time, high substrate cost, and achieve background Low luminescence, rapid reaction and good reagent stability

Inactive Publication Date: 2018-06-12
太原瑞盛生物科技有限公司
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN106199002 A (December 2016) discloses a chemiluminescent immunoassay kit for detecting ST2, its preparation method and application. The kit uses a microwell plate to coat the ST2 antibody, and alkaline phosphatase to label the ST2 antibody. The main disadvantage of using alkaline phosphatase to realize the ST2 index of chemiluminescent immunoassay by the double-antibody sandwich method is: the time for the substrate to reach the plateau is long, and the cost of the substrate is high, resulting in high detection cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] The present invention provides a human-soluble ST2 magnetic particle chemiluminescence detection kit and a preparation method. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention will be further described in detail below.

[0023] The present invention provides a magnetic particle chemiluminescence detection kit for human soluble ST2 and a preparation method thereof, wherein the magnetic particle chemiluminescent detection kit for human soluble ST2 uses magnetic particles coupled with soluble ST2 capture antibodies, Acridinium ester labeled soluble ST2 detection antibody. The kit also includes soluble ST2 calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution for the above-mentioned acridinium ester action.

[0024] Specifically, the magnetic particle-coupled suspension of the present invention is composed of magnetic particles coup...

Embodiment 1

[0034] Embodiment 1: The formation and preparation method of the magnetic particle chemiluminescence kit for detecting human soluble ST2 includes the following steps:

[0035] 1. The composition of the kit:

[0036] A magnetic particle chemiluminescence kit for detecting human soluble ST2 is assembled so that it contains the following components:

[0037] Soluble ST2 monoclonal antibody coupled to magnetic particles;

[0038] Another soluble ST2 monoclonal antibody labeled with acridinium ester;

[0039] Soluble ST2 series standard solution;

[0040] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0041] Cleaning fluid.

[0042] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Soluble ST2 Monoclonal Antibody

[0043] (1) Take 1 mg of carboxyl magnetic particles in a 0.5 mL centrifuge tube, add 200 μL of MES buffer solution with a concentration of 0.1 mol / L, vortex and mix, place on a magnetic stand, and let it ...

Embodiment 2

[0063] Embodiment 2: The steps for detecting using the magnetic particle chemiluminescence detection kit of human soluble ST2 are as follows:

[0064] (1) Add 50 μL of the sample to be tested into the cuvette, then add 150 μL of magnetic particle coupling suspension, shake and mix, and incubate at 37°C for 8 min.

[0065] (2) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0066] (3) Add 150 μL of acridinium ester marker into the cuvette, shake to mix, and incubate at 37°C for 7 min.

[0067] (4) Separation, washing 3 times, fully shaking the washed reaction vessel to disperse the magnetic particles.

[0068] (5) Add 100 μL of chemiluminescence pre-excitation solution A, add 100 μL of chemiluminescence excitation solution B after 1 s, and measure its relative luminous intensity. The content of soluble ST2 in the sample is proportional to its relative luminous intensity.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Analytical sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a magnetic particle chemiluminescence detection kit for human soluble ST2 and a preparation method. The kit comprises magnetic particles coupled with a soluble ST2 capture antibody, an acridinium ester-labeled soluble ST2 detection antibody, a soluble ST2 calibrator, a chemiluminescent pre-excitation solution A, a chemiluminescent excitation solution B, and a cleaning solution. The kit disclosed by the invention adopts magnetic separation chemiluminescence technology as a detection means, and simultaneously combines acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, high specificity, accuracy and rapidity, and short detection time, the detection result has higher accuracy and repeatability, and the kit can be suitable for various illumination detecting instruments.

Description

technical field [0001] The invention belongs to the technical field of immunodetection and analysis, in particular to a human soluble ST2 immunomagnetic particle chemiluminescent detection kit and a preparation method. Background technique [0002] Heart failure is a complex clinical syndrome characterized by ventricular dysfunction, neuroendocrine activation, and abnormal distribution of peripheral blood flow. It is the terminal stage of various cardiovascular diseases, and its morbidity and mortality are high. Clinically, the symptoms and signs of heart failure are poorly specific, and auxiliary examinations such as echocardiography, X-ray, and invasive hemodynamics also have limitations and are limited by objective conditions. Therefore, it is still necessary to seek an objective, reliable, simple, cheap, easy-to-follow-up and suitable method for dynamic observation as a supplementary method to improve the sensitivity and specificity of diagnosis and help judge the sever...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/53G01N33/543G01N21/76
CPCG01N33/53G01N21/76G01N33/54326
Inventor 常燕胡雪婷曹晶刘丽青杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com