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High-yield cotton breeding method

A cotton seed and high-yield technology, applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of limited number of cotton varieties and difficulty in showing good traits, and achieve the goal of increasing the mutation rate and the survival rate of high-distortion plants Effect

Inactive Publication Date: 2018-04-13
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of known cotton varieties is limited, coupled with the limitation of parental traits, the traits of the bred cotton varieties are generally improved parental traits, and it is difficult to appear new good traits

Method used

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  • High-yield cotton breeding method

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Effect test

Embodiment 1

[0029] A kind of high-yield cotton breeding method provided by the invention specifically comprises the following steps:

[0030] S1, collecting high-yield Bainong 8602 cotton seeds;

[0031] Cotton seeds are required to be full-bodied and disease-free. Bainong No. 8602 variety is characterized by resistance to Fusarium wilt, Verticillium wilt and cotton bollworm.

[0032] S2, mutagenesis treatment:

[0033] Soak the cotton seeds in the mutagen for 5 minutes, then soak them in a 0.05% EMS solution by volume fraction for 4 hours, take out the seeds and irradiate them to obtain the mutagenic seeds;

[0034] The mutagen is prepared by mixing the following volume percentage substances: 0.1% EMS, 0.1% nitrous acid, and the solvent is 0.1mol / L, pH 3 phosphate buffer;

[0035] The specific steps of the mutagenesis treatment are as follows: soak the cotton seeds in the mutagen for 5 minutes; take out the cotton seeds and blot the water on the seed surface, then soak them in 0.05% EM...

Embodiment 2

[0044] The operation is basically the same as in Example 1, the difference being S2, the mutagenesis treatment steps are as follows:

[0045] Soak the cotton seeds in the mutagen for 30 minutes, then soak them in a 0.1% EMS solution by volume fraction for 3 hours, take out the seeds and irradiate them to obtain the mutagenic seeds;

[0046] The mutagen is prepared by mixing the following substances by volume percentage: 0.5% EMS, 0.3% nitrous acid, and the solvent is 0.1mol / L, pH 3 phosphate buffer;

[0047] The specific steps of the mutagenesis treatment are as follows: soak the cotton seeds in the mutagen for 30 minutes; take out the cotton seeds and blot the water on the surface of the seeds, and then soak them in a 0.1% EMS solution by volume fraction for 3 hours, take out the cotton seeds, and blot the surface of the seeds dry moisture, and put into a ziplock bag; finally use 60 Coγ-ray radiation, the radiation dose rate is 1Gy / min, and the radiation dose is 30Gy, to obt...

Embodiment 3

[0049] The operation is basically the same as in Example 1, the difference being S2, the mutagenesis treatment steps are as follows:

[0050] Soak the cotton seeds in the mutagen for 15 minutes, then soak them in a 0.08% EMS solution by volume fraction for 3.5 hours, and finally take out the seeds and treat them with ray radiation to obtain the mutagenic seeds;

[0051] The mutagen is prepared by mixing the following substances by volume percentage: 0.4% EMS, 0.2% nitrous acid, and the solvent is 0.1mol / L, pH 3 phosphate buffer;

[0052] The specific steps of the mutagenesis treatment are as follows: soak the cotton seeds in the mutagen for 15 minutes; take out the cotton seeds and blot the water on the surface of the seeds, and then soak them in a 0.08% EMS solution by volume fraction for 3.5 hours, take out the cotton seeds, and blot the seeds dry. moisture on the surface, and put into a ziplock bag; finally use 60 Coγ-ray radiation, the radiation dose rate is 1Gy / min, and th...

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Abstract

The invention relates to the technical field of cotton breeding and discloses a high-yield cotton breeding method. According to the high-yield cotton breeding method, a mutant is generated through a mutagenesis method; then the mutant is hybridized with parents with good properties, so that new good properties can be increased in a new variety. According to the embodiment of the invention, the high-yield and disease-resisting new variety is cultured; under the common effect of a single 0.05 to 0.1 percent EMS mutagen, compound type 0.1 to 0.5 percent EMS, a 0.1 to 0.3 percent nitrous acid mutagen and rays, cotton seeds have relatively high distortion rate and distorted plant survival rate; changes in a process from seedlings to plants are observed and distorted plants have a new phenotype,so that the mutagenesis method provided by the invention can be used for effectively improving the mutation rate of the cotton seeds and a foundation is laid for the new phenotype of the variety.

Description

technical field [0001] The invention belongs to the technical field of cotton breeding, and in particular relates to a high-yield cotton breeding method. Background technique [0002] There are three major cotton areas in my country, which are (1) the cotton area in the Yellow River Basin, which includes Shandong, Hebei, Henan and other places; (2) the cotton area in the Yangtze River Basin, which includes Hunan, Hubei, Jiangsu, Anhui and other places; (3) ) Xinjiang cotton area. Cotton is an important economic crop and strategic resource, and the cotton industry occupies an important position in the national economy. Aggregating the achievements of cotton breeding with various excellent traits has a vital scientific and technological contribution to the increase of cotton production and the prosperity of the industry. Yield, disease resistance, and fiber quality have always been the first choice for cotton breeding. As an economical agricultural and sideline product, the y...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H1/02
CPCA01H1/02A01H1/06
Inventor 王青连李成奇张新王园园孙润润付远志董娜张志勇胡根海张金宝晁毛妮薛惠云张晓红秦腾飞谭阳光韩振甫韦春艳董涛
Owner HENAN INST OF SCI & TECH
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