Fungicidal composition containing Bacillus amyloliquefaciens and preparation method and use thereof

A bactericide composition and technology of Bacillus amyloliquefaciens, which are applied in the directions of botanical equipment and methods, bactericides, biocides, etc., can solve the problems of tobacco production loss, mass occurrence of tobacco diseases, etc., reduce the cost of medication and achieve good disinfection effect. , the effect of promoting growth and development

Inactive Publication Date: 2018-04-17
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These diseases can lead to a large number of tobacco diseases at the seedling and field stages, causing serious losses to tobacco production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of a bactericide composition containing bacillus amyloliquefaciens, comprising the following steps:

[0020] (1) Mix prochloraz and azoxystrobin with methanol to make 1×10 3 µg / ml stock solution, stored at 4°C for later use;

[0021] (2) Bacillus amyloliquefaciens X60 ( Bacillus amyloliquefaciens X60), preservation number: CCTCC M2013375, in NB culture medium (preparation method: 3g beef extract, 10g peptone, add deionized water to 1L, pH 6.8-7.2, sterilized at 120°C for 20 minutes) 30°C 180rpm shaking culture 36-48 hours, then centrifuge at 6000rpm for 10 minutes, take X60 wet bacteria and bacteria preservation solution (containing 0.01 mol / L phosphate buffer, 0.001 mol / L Tween 40 non-ionic surfactant), according to mass volume The ratio is 1:50 to prepare the bacterial agent, and the concentration of the finished bacterial agent is 1×10 10 -1×10 12 CFU / ml;

[0022] (3) Prepare the final test concentration containing prochloraz 10µg / ml, azoxy...

Embodiment 2

[0024] A preparation method of a bactericide composition containing bacillus amyloliquefaciens, comprising the following steps:

[0025] (1) Mix prochloraz and azoxystrobin with methanol to make 1×10 3 µg / ml stock solution, stored at 4°C for later use;

[0026] (2) Bacillus amyloliquefaciens X60 ( Bacillus amyloliquefaciens X60), preservation number: CCTCC M2013375, in NB culture medium (preparation method: 3g beef extract, 10g peptone, add deionized water to 1L, pH 6.8-7.2, sterilized at 120°C for 20 minutes) 30°C 180rpm shaking culture 36-48 hours, then centrifuge at 6000rpm for 10 minutes, take X60 wet bacteria and bacteria preservation solution (containing 0.01 mol / L phosphate buffer, 0.001 mol / L Tween 40 non-ionic surfactant), according to mass volume The ratio is 1:50 to prepare the bacterial agent, and the concentration of the finished bacterial agent is 1×10 10 -1×10 12 CFU / ml;

[0027] (3) Prepare the final test concentration containing prochloraz 8 µg / ml, azoxy...

Embodiment 3

[0029] A preparation method of a bactericide composition containing bacillus amyloliquefaciens, comprising the following steps:

[0030] (1) Mix prochloraz and azoxystrobin with methanol to make 1×10 3 µg / ml stock solution, stored at 4°C for later use;

[0031] (2) Bacillus amyloliquefaciens X60 ( Bacillus amyloliquefaciensX60), preservation number: CCTCC M2013375, in NB culture medium (preparation method: 3g beef extract, 10g peptone, add deionized water to 1L, pH 6.8-7.2, sterilized at 120°C for 20 minutes) 30°C 180rpm shaking culture 36-48 hours, then centrifuge at 6000rpm for 10 minutes, take X60 wet bacteria and bacteria preservation solution (containing 0.01 mol / L phosphate buffer, 0.001 mol / L Tween 40 non-ionic surfactant), according to mass volume The ratio is 1:50 to prepare the bacterial agent, and the concentration of the finished bacterial agent is 1×10 10 -1×10 12 CFU / ml;

[0032] (3) Prepare the final test concentration containing prochloraz 5 µg / ml, azoxys...

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PUM

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Abstract

The invention discloses a fungicidal composition containing Bacillus amyloliquefaciens and a preparation method and a use thereof. Prochloraz and azoxystrobin are respectively prepared into mother liquor of 1*103mug / ml with methanol and preserved at low temperature of 4 DEG C for later use; a Bacillus amyloliquefaciens strain X60 is subjected to shaking culture in an NB culture medium at 30 DEG Cand 180rpm for 36-48 hours, then wet cells of the Bacillus amyloliquefaciens strain X60 are collected after centrifugation at 6000-10000rpm for 10 minutes, the wet cells of the X60 and cell storage liquid are taken and prepared into a fungicide according to the mass / volume ratio of 1: 50, and the living cell concentration in the fungicide finished product is 1*1010-1*1012CFU / ml; and the prochloraz, the azoxystrobin and the wet cells of the Bacillus amyloliquefaciens are mixed, and the prepared composition contains 10-40Mmug / ml of the prochloraz, 5 to 10Mmug / ml of the azoxystrobin and 1*104-1*1010 CFU / ml of the Bacillus amyloliquefaciens. The fungicidal composition disclosed by the invention can process pathogenic fungi carried by tobacco seeds and has the advantages of relatively good disease prevention effect, environment friendliness and capability of significantly promoting the growth of tobacco seedlings.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a bactericide composition containing Bacillus amyloliquefaciens, and to the preparation of the bactericide composition containing Bacillus amyloliquefaciens method, and the use of the fungicide composition containing Bacillus amyloliquefaciens in the disinfection of tobacco seeds. Background technique [0002] Tobacco seeds are one of the most important ways to spread tobacco pathogens. Tobacco seed-borne pathogens usually exist on the surface or inside of seeds, or directly or indirectly mix among seeds with various diseased residues. These pathogenic bacteria are often the source of primary infection of tobacco diseases. The reported pathogenic bacteria carried by tobacco seeds are Alternaria spp. ( Alternaria sp . ), Phomopsis ( Phomopsis sp . ), Acremonium ( Acremonium sp . ), Podospora ( Zopfiella sp . ), Aspergillus ( Aspergillus sp . ), Fusarium ( F...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N43/54A01N47/38A01P1/00A01P3/00
CPCA01N43/54A01N47/38A01N63/00
Inventor 汪汉成李文红李丽翠陈兴江王进蔡刘体陆宁涂永高丁福章
Owner GUIZHOU TOBACCO SCI RES INST
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