Leavening agents
A technology of liquid fermentation agent and composition, which is applied in the direction of microorganisms, biochemical equipment and methods, fungi, etc., and can solve the problems of poor aftertaste and poor aroma
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Embodiment 1
[0145] Embodiment 1: leavening agent
[0146] Strains: Saccharomyces bouidin Kazakhstan (MUCL 54530), Saccharomyces parvum (ATCC 22034), Saccharomyces thermotolerant (MUCL 55817) and two strains of Saccharomyces cerevisiae (regular baker's yeast and organic regular baker's yeast)
[0147] Inoculum: Yeast strains were kept at -70°C and maintained on solid YPD medium (20 g / l peptone; 10 g / l yeast extract; 20 g / l agar). Isolated colonies of each strain were inoculated into the first flask containing 25 mL of liquid YPD medium (20 g / l peptone; 10 g / l yeast extract) and maintained at 100 rpm and 30° C. for 24 hours. 5ml of this culture was inoculated into 200mL of liquid medium (bacto-peptone 3.5g / l; yeast extract 3g / l; KH 2 PO 4 2g / l; MgSO 4 ·7H 2 O 1.83g / l; (NH 4 ) 2 SO 4 1 g / l; D-glucose 20 g / l; sucrose 55 g / l; penicillin 0.0004 g / l; pH=4.8) in two flasks and kept stirring at room temperature for 48 hours.
[0148] The liquid leavening agent was obtained after 3 consec...
Embodiment 2
[0162] Embodiment 2: baking test
[0163] Country bread was made using different liquid leavening agents obtained by the method of Example 1. The properties of the leavening agents are shown in Table 2 and the composition of the bread dough is shown in Table 3.
[0164] Table 2: Composition of liquid leavening agent
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[0167] Table 3: Dough Composition
[0168]
[0169] The ingredients were mixed in a dough mixer (Diosna SP24) for 5 minutes on low speed and 6 minutes on high speed. The temperature in the bread oven is about 25°C and the humidity is about 55%. The temperature of the water was about 12°C and the temperature of the dough was about 27°C. After 20 minutes of overall fermentation at room temperature, the dough was divided into 800 g pieces, shaped into round shapes and subjected to the final proofing step in a Koma fermentation room (70min, 30°C, 90% relative humidity) and then in a Miwe Condo oven Bake with steam at 230°C for 35min....
Embodiment 3
[0180] Example 3: Characterization of Aroma
[0181] A series of liquid leavening agents were prepared according to the method of Example 1 using different yeast strains and different carbon sources. Table 4 summarizes their composition and properties.
[0182] Table 4: Composition of liquid leavening agent
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[0185] Bread was prepared as in Example 2 using these leavening agents.
[0186] Flavor comparison by MS nasal technique
[0187] Analysis of liquid leavening agents and aroma of bread by MS nasal technique. The MS nasal apparatus is equipped with an MPS-2W autosampler The gas chromatograph mass spectrometer HP 7890A / 5975C (Agilent )constitute. Will Software (G1701 BA, version 01.00, Agilent )with Multivariate analysis software (9.8, Oslo, Norway) loaded into the GC-MS system.
[0188] Sample weights of 1 g of each leavening agent and each bread were weighed in 20 ml glass vials, closed with magnetic caps with silicone / PTFE sep...
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