Application of caffeic acid to prepare anti-HPV-virus-infection medicine
A technology of HPV virus and caffeic acid, which is applied in the field of biomedicine, can solve the problems of research and development failure, inability to treat, and limitations, and achieve the effect of low price and long application history
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[0029] The present invention will be further described below in conjunction with experiments, but the protection scope of the present invention is not limited thereto.
[0030] Experiment 1: Pseudovirus infection model to detect the inhibitory effect of caffeic acid on HPV pseudovirus
[0031] experimental method:
[0032] 1) Gradually dilute caffeic acid, mix and incubate with HPV6 / HPV16 / HPV18 pseudovirus liquid in equal volumes for 30 minutes, then add it to the pre-inoculated cell culture plate, set virus control wells and cell blank control wells;
[0033] 2) Change the fresh medium after 24 hours, and detect the expression of luciferase in the cells with a luciferase detection kit 48 hours after virus infection.
[0034] Experimental results such as figure 1As shown, caffeic acid can effectively inhibit the three subtypes of HPV pseudoviruses from infecting cells in a concentration-dependent manner. IC for HPV6, 16, and 18 viruses 50 They were 12.06±0.40, 12.92±0.54, a...
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