Preparation method of stable lactobacillus microecological preparation
A technology of micro-ecological preparations and lactic acid bacteria, applied in the direction of bacteria, etc., can solve the problems of high cost of fermentation tanks, unstable products, and high transportation costs, and achieve the effects of rich metabolites, stable storage time, simple cultivation equipment and operation.
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Embodiment 1
[0026]A preparation method of a stable lactic acid bacteria microecological preparation, using cultured animals, cultured water and bottom mud as bacteria sources, isolating lactic acid bacteria strains by coating solid MRS medium after enriching in MRS liquid medium; and then separating the The lactic acid bacteria strains were purified and selected to produce three lactic acid bacteria strains with large calcium-dissolving circles, fast growth and strong vitality; then the lactic acid bacteria strains selected after purification and breeding were expanded and cultivated to prepare composite seed liquid; finally, the composite seed liquid Composite lactic acid bacteria probiotics can be obtained by inoculation and fermentation:
[0027] (1) With cultured animals, cultured water and sediment as the source of bacteria, lactic acid bacteria strains are isolated by coating solid MRS medium after enrichment in MRS liquid medium;
[0028] (2) Purify and select three lactic acid bac...
Embodiment 2
[0039] A preparation method of a stable lactic acid bacteria microecological preparation, using cultured animals, cultured water and bottom mud as bacteria sources, isolating lactic acid bacteria strains by coating solid MRS medium after enriching in MRS liquid medium; and then separating the Purify and select four lactic acid bacteria strains with large calcium-dissolving circles, fast growth and strong vitality; then expand the culture of the selected lactic acid bacteria strains after purification and breeding to prepare a composite seed liquid; finally, the composite seed liquid Composite lactic acid bacteria probiotics can be obtained by inoculation and fermentation:
[0040] (1) With cultured animals, cultured water and sediment as the source of bacteria, lactic acid bacteria strains are isolated by coating solid MRS medium after enrichment in MRS liquid medium;
[0041] (2) Purify and select four lactic acid bacteria strains with large calcium-dissolving circle, fast gr...
Embodiment 3
[0052] A preparation method of a stable lactic acid bacteria microecological preparation, using cultured animals, cultured water and bottom mud as bacteria sources, isolating lactic acid bacteria strains by coating solid MRS medium after enriching in MRS liquid medium; and then separating the The lactic acid bacteria strains were purified and selected to produce five lactic acid bacteria strains with large calcium-dissolving circles, fast growth and strong vitality; then the lactic acid bacteria strains selected after purification and breeding were expanded and cultivated to prepare composite seed liquid; finally, the composite seed liquid Composite lactic acid bacteria probiotics can be obtained by inoculation and fermentation:
[0053] (1) With cultured animals, cultured water and sediment as the source of bacteria, lactic acid bacteria strains are isolated by coating solid MRS medium after enrichment in MRS liquid medium;
[0054] (2) The isolated lactic acid bacteria strai...
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