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Preparation method of stable lactobacillus microecological preparation

A technology of micro-ecological preparations and lactic acid bacteria, applied in the direction of bacteria, etc., can solve the problems of high cost of fermentation tanks, unstable products, and high transportation costs, and achieve the effects of rich metabolites, stable storage time, simple cultivation equipment and operation.

Inactive Publication Date: 2018-05-18
郑州神草生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, most of the products coated with lactic acid bacteria probiotics are expensive, and the cost of use is very high; while the products of lactic acid bacteria probiotics are relatively cheap, but most of the products are very unstable and the number of viable bacteria is low. The storage period is very short, and the transportation cost is high; currently, most of the solid and liquid lactic acid bacteria probiotics are fermented by fermentation tanks. On the one hand, the production process is strict and requires professional technicians to operate; higher cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026]A preparation method of a stable lactic acid bacteria microecological preparation, using cultured animals, cultured water and bottom mud as bacteria sources, isolating lactic acid bacteria strains by coating solid MRS medium after enriching in MRS liquid medium; and then separating the The lactic acid bacteria strains were purified and selected to produce three lactic acid bacteria strains with large calcium-dissolving circles, fast growth and strong vitality; then the lactic acid bacteria strains selected after purification and breeding were expanded and cultivated to prepare composite seed liquid; finally, the composite seed liquid Composite lactic acid bacteria probiotics can be obtained by inoculation and fermentation:

[0027] (1) With cultured animals, cultured water and sediment as the source of bacteria, lactic acid bacteria strains are isolated by coating solid MRS medium after enrichment in MRS liquid medium;

[0028] (2) Purify and select three lactic acid bac...

Embodiment 2

[0039] A preparation method of a stable lactic acid bacteria microecological preparation, using cultured animals, cultured water and bottom mud as bacteria sources, isolating lactic acid bacteria strains by coating solid MRS medium after enriching in MRS liquid medium; and then separating the Purify and select four lactic acid bacteria strains with large calcium-dissolving circles, fast growth and strong vitality; then expand the culture of the selected lactic acid bacteria strains after purification and breeding to prepare a composite seed liquid; finally, the composite seed liquid Composite lactic acid bacteria probiotics can be obtained by inoculation and fermentation:

[0040] (1) With cultured animals, cultured water and sediment as the source of bacteria, lactic acid bacteria strains are isolated by coating solid MRS medium after enrichment in MRS liquid medium;

[0041] (2) Purify and select four lactic acid bacteria strains with large calcium-dissolving circle, fast gr...

Embodiment 3

[0052] A preparation method of a stable lactic acid bacteria microecological preparation, using cultured animals, cultured water and bottom mud as bacteria sources, isolating lactic acid bacteria strains by coating solid MRS medium after enriching in MRS liquid medium; and then separating the The lactic acid bacteria strains were purified and selected to produce five lactic acid bacteria strains with large calcium-dissolving circles, fast growth and strong vitality; then the lactic acid bacteria strains selected after purification and breeding were expanded and cultivated to prepare composite seed liquid; finally, the composite seed liquid Composite lactic acid bacteria probiotics can be obtained by inoculation and fermentation:

[0053] (1) With cultured animals, cultured water and sediment as the source of bacteria, lactic acid bacteria strains are isolated by coating solid MRS medium after enrichment in MRS liquid medium;

[0054] (2) The isolated lactic acid bacteria strai...

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PUM

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Abstract

The invention provides a preparation method of a stable lactobacillus microecological preparation and belongs to the field of lactobacillus fermentation. The preparation method includes: using breeding animal, breeding water body and bottom mud as bacteria sources to separate out lactobacillus strains; purifying the lactobacillus strains to breed 2-5 lactobacillus strains with large calcium dissolving circle, high growing speed and high vitality; performing enlarging culture on the lactobacillus strains which are bred to prepare composite seed liquid; inoculating the composite seed liquid forfermentation to obtain a composite lactobacillus microecological preparation. Compared with the prior art, the preparation method has the advantages that the lactobacillus microecological preparationis quite simple in both culture equipment and operation and quite stable in storage time when compared with that of single-strain fermentation water agents.

Description

technical field [0001] The invention relates to the field of lactic acid bacteria fermentation, in particular to a preparation method of a stable lactic acid bacteria microecological preparation. Background technique [0002] With the rapid development of the aquaculture industry, lactic acid bacteria preparations have attracted more and more attention because of their natural, non-toxic, no side effects, no pollution, no residue, safe and reliable advantages. As a beneficial "bioremediation agent" for purifying water, the application of lactic acid bacteria preparations in aquaculture industry has attracted more and more attention. Splash on bodies of water. Among them, the research on mixing and feeding and fermented feed focuses on the inhabitation of lactic acid bacteria in the intestines and stomach of aquatic animals, and the regulation of animal intestinal flora structure and changes in feed structure. Sprinkling to regulate water quality is closely related to the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 不公告发明人
Owner 郑州神草生物技术有限公司
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