Construction method and application of animal model suffering from fetal-origin adult osteoporosis

An osteoporosis and animal model technology, applied in medical preparations containing active ingredients, organic active ingredients, pharmaceutical formulations, etc., can solve the problems of slow progress in laboratory research and lack of model establishment of fetal-derived osteoporosis. , to achieve the effect of improving the quality of the population

Inactive Publication Date: 2018-06-01
WUHAN UNIV
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Problems solved by technology

However, the current research on fetal osteoporosis is limited to epidemiological research, and the progress of laboratory research is slow. The reason may be related to the lack of a standardized and stable fetal osteoporosis model.

Method used

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  • Construction method and application of animal model suffering from fetal-origin adult osteoporosis
  • Construction method and application of animal model suffering from fetal-origin adult osteoporosis
  • Construction method and application of animal model suffering from fetal-origin adult osteoporosis

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Embodiment Construction

[0029] The features and advantages of the present invention can be further understood through the following detailed description in conjunction with the accompanying drawings. The examples provided are only illustrative of the method of the present invention and do not limit the rest of the present disclosure in any way.

[0030] [Example 1] Occurrence of osteoporosis in offspring rats as adults caused by caffeine exposure during pregnancy

[0031] 1. Experimental animals

[0032] SPF grade healthy Wistar rats were purchased from Hubei Provincial Center for Disease Control and Prevention, animal license number: SCXK (E) 2008-2010. This study was approved by the Ethics Committee of Wuhan University Medical Center, and was carried out in strict accordance with the relevant treatment guidelines of international laboratory animal protection certification and evaluation institutions.

[0033] The experimental animals were kept in a barrier environment with a temperature of 22-25°...

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Abstract

The invention discloses a construction method and application of an animal model suffering from fetal-origin adult osteoporosis. The construction method comprises: performing intragastric administration with 30-120 mg/kg/d of caffeine and 4-4.6 g/kg/d of ethanol on a rodent which has been pregnant for 9-20 days, performing subcutaneous injection with 1-2 mg/kg/d of nicotine or 0.2-1.2 mg/kg/d of dexamethasone, and allowing the mother rat to naturally give birth to offspring. After the offspring is naturally cultured to an adult, the phenomenon of bone mass peak reduction and osteoporosis in the elder period, which meets the clinical diagnosis standard, shows up, thereby obtaining a stable animal model suffering from fetal-origin adult osteoporosis. The animal model has symptom and pathologic change similar to that of human adult osteoporosis, and has important value for screening of explosion in multiple adverse intrauterine environments and research of pathogenesis and control strategy of fetal-origin adult osteoporosi.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing an animal model of fetal-derived adult osteoporosis and its application. Background technique [0002] Osteoporosis is a pathological change in bone metabolism, characterized by decreased bone formation and increased bone resorption, which can lead to loss of bone mass, increased fragility of bone tissue, and easy fractures throughout the body [1]. Previous studies have found that low birth weight infants have an increased risk of developing diseases such as osteoporosis as adults, and the reason for this is closely related to the adverse environment of the mother during pregnancy [2]. It is suggested that osteoporosis has an intrauterine origin. However, the current research on fetal osteoporosis is limited to epidemiological research, and the progress of laboratory research is slow. The reason may be related to the lack of a standard...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/522A61K31/045A61K31/465A61K31/573A61K49/00
Inventor 陈廖斌汪晖文印宪潘正启上官杨帆肖浩
Owner WUHAN UNIV
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