Method for culturing spirulina containing rich arginine

A cultivation method and technology of spirulina, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of affecting the output of arginine in spirulina, difficult industrialized production, and failure to achieve health care, etc. The effect of improving the level of high value-added industrial chain, low training cost and improving product output

Active Publication Date: 2018-06-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Spirulina cells contain arginine, but under normal culture conditions, the content of arginine in spirulina is very small, less than 3% of the dry weight of cells, which cannot achieve the function of health care, and also affects the production of arginine in spirulina , which increases the cost of subsequent processing and extraction, making it difficult for industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for culturing spirulina containing rich arginine
  • Method for culturing spirulina containing rich arginine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] To investigate the accumulation of arginine in Spirulina under sulfur-limited conditions.

[0022] The algae cultivation and arginine accumulation processes were carried out in a 500mL cylindrical bubbling photobioreactor (glass material, diameter 50mm, height 400mm).

[0023] 1) Cultivation of algal species

[0024] Spirulina cells are cultured in a nutrient-rich medium (Zarrouk) prepared with deionized water containing: NaHCO per liter of water 3 16.8g, K 2 HPO 4 0.5g, NaNO 3 2.5g, K 2 SO 41g, NaCl 1g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.04g, FeSO 4 ·7H 2 O 0.01g, H 3 BO 3 2.86mg, MnCl 2 4H 2 O 1.86mg, ZnSO 4 ·7H 2 O0.22mg, Na 2 MoO 4 2H 2 O 0.39mg, CuSO 4 ·5H 2 O 0.08mg. Initial inoculation density OD 560 The temperature is 0.5, the culture temperature is 30±2°C, and the light is continuously illuminated. The light is irradiated from one side of the columnar bubble reactor, and the light receiving area is 0.013m 2 , light intensity 150μm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to culture of spirulina for arginine accumulation, in particular to culture of spirulina by controlling addition of nutritive salt to allow the spirulina to accumulate arginine.Spirulina cells cultured to an exponential growth phase are transferred into a sulfur-limited culture medium, subjected to natural lighting or artificial lighting and cultured to a stable phase, spirulina cells are harvested, and the content and the yield of arginine of the spirulina reach 5%-8% of the dry weight of spirulina and 65 mg / L-140 mg / L and are 2-3 times and 20%-40% higher than those ofspirulina under a rich nutrient condition respectively. The spirulina containing rich arginine is produced quickly and efficiently through photoautotrophic carbon fixation. The related culture methodhas the advantages that the nutritive salt consumption is low, the production cost is low, the yield and the content of the obtained arginine are high, and the method is coupled with the carbon emission reduction process and the like and has good economic and environmental benefits.

Description

technical field [0001] The invention relates to accumulating arginine by cultivating spirulina, in particular to cultivating spirulina by controlling the addition of nutrient salts and adopting appropriate inoculation density, light, temperature and pH value to accumulate arginine. It can be used in the cultivation and production of arginine-rich spirulina, as well as in related fields such as food, health care products, medicine, and fine chemical manufacturing using arginine as raw material. Background technique [0002] Arginine is a semi-essential amino acid that plays important physiological, metabolic and nutritional roles in the human body. The specific functions are as follows: arginine is a component in the ornithine cycle, it can increase the activity of arginase in the liver, which is beneficial to hyperammonemia, liver dysfunction, etc.; arginine is a component of sperm protein The main ingredient has the effect of promoting the quality of sperm and improving th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C12R1/01
CPCC12N1/20C12N1/38
Inventor 薛松姚长洪刘瑞国曹旭鹏陆洪斌姜君鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products