Method for culturing spirulina containing rich arginine

Abstract

The invention relates to culture of spirulina for arginine accumulation, in particular to culture of spirulina by controlling addition of nutritive salt to allow the spirulina to accumulate arginine.Spirulina cells cultured to an exponential growth phase are transferred into a sulfur-limited culture medium, subjected to natural lighting or artificial lighting and cultured to a stable phase, spirulina cells are harvested, and the content and the yield of arginine of the spirulina reach 5%-8% of the dry weight of spirulina and 65 mg / L-140 mg / L and are 2-3 times and 20%-40% higher than those ofspirulina under a rich nutrient condition respectively. The spirulina containing rich arginine is produced quickly and efficiently through photoautotrophic carbon fixation. The related culture methodhas the advantages that the nutritive salt consumption is low, the production cost is low, the yield and the content of the obtained arginine are high, and the method is coupled with the carbon emission reduction process and the like and has good economic and environmental benefits.

Description

technical field [0001] The invention relates to accumulating arginine by cultivating spirulina, in particular to cultivating spirulina by controlling the addition of nutrient salts and adopting appropriate inoculation density, light, temperature and pH value to accumulate arginine. It can be used in the cultivation and production of arginine-rich spirulina, as well as in related fields such as food, health care products, medicine, and fine chemical manufacturing using arginine as raw material. Background technique [0002] Arginine is a semi-essential amino acid that plays important physiological, metabolic and nutritional roles in the human body. The specific functions are as follows: arginine is a component in the ornithine cycle, it can increase the activity of arginase in the liver, which is beneficial to hyperammonemia, liver dysfunction, etc.; arginine is a component of sperm protein The main ingredient has the effect of promoting the quality of sperm and improving th...

AI Technical Summary

Problems solved by technology

Spirulina cells contain arginine, but under normal culture conditions, the content of arginine in spirulina is very small, less than 3% of the dry weight of cells, which cannot achieve the function of health care, and also affects the production of arginine in spirulina , which increases the cost of subsequent processing and extraction, making it difficult for industrial production

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