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A kind of immunopotentiator and its application

An immune enhancer and immune enhancement technology are applied in the field of swine foot-and-mouth disease vaccine compositions to achieve the effects of enhancing the level of cellular immune response, good immune effect and broad application prospects

Active Publication Date: 2020-06-09
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with B cell epitopes and Th cell epitopes of FMD, there are few studies on cytotoxic T cell CTL epitopes. Some studies have confirmed that FMDV-specific CTL epitopes can induce cellular immune responses in the body. Therefore, The development of immune enhancers of CTL epitopes has important application significance for cell-mediated anti-FMDV immunity

Method used

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  • A kind of immunopotentiator and its application
  • A kind of immunopotentiator and its application
  • A kind of immunopotentiator and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1, Screening of CTL epitope polypeptides

[0036] It is predicted that after the polypeptide immunizes the body, the polypeptide antigen presented by MHC class I molecules can stimulate the body to produce memory T cells. Memory T cells induce the production of cytokines when the polypeptide antigen stimulates T cells again. Whether the predicted polypeptide is a CTL epitope polypeptide can be evaluated and analyzed through the detection of cytokine levels and biostatistical analysis.

[0037] 1. Prediction of peptides:

[0038] 10 predicted peptides ZM1501-ZM15010 were predicted and chemically synthesized from the website, and the sequence information is shown in the sequence list. 20mM Tris-HCl (pH 8.0) and 50mM NaCl buffer solutions were used to dissolve the predicted polypeptides respectively, and all 10 predicted polypeptides were prepared into a solution with a concentration of 1 mg / ml. Each predicted polypeptide was mixed with 61 adjuvants at a volum...

Embodiment 2

[0065] Example 2, Application of CTL Epitope-containing Polypeptide Vaccine Composition

[0066] 1. Three functional CTL epitope polypeptides were screened in Example 1: ZM1501, ZM1502 and ZM1503, dissolved in 20 mM Tris-HCl (pH 8.0), 50 mM NaCl buffer solution. The three CTL epitope polypeptides were prepared into a solution with a concentration of 1 mg / ml. in,

[0067] ZM1501 sequence: SADPVTATV (sequence 1 in the sequence listing)

[0068] ZM1501 sequence: ALDNTTNPT (sequence 2 in the sequence listing)

[0069] ZM1501 sequence: LM QTPSHTL (sequence 3 in the sequence listing)

[0070] According to the immunization dose and immunization group, take each CTL epitope polypeptide or prepare a mixed solution and mix it with the O / Mya98 / XJ / 2010 inactivated antigen containing the immunization dose, incubate overnight at 4°C, and mix the obtained mixed solution with Montanide ISA 206 adjuvant The porcine foot-and-mouth disease vaccine composition is prepared.

[0071] As show...

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Abstract

The invention provides an immune enhancer, a porcine foot-and-mouth disease vaccine composition containing the immune enhancer and its application. The immune enhancer includes three porcine foot-and-mouth disease virus CTL epitope polypeptides, which are one of the polypeptide shown in sequence 1 in the sequence listing, the polypeptide shown in sequence 2 in the sequence listing and the polypeptide shown in sequence 3 in the sequence listing Or any combination of two or more. The porcine foot-and-mouth disease vaccine composition comprises an immune enhancer and a porcine foot-and-mouth disease antigen. The immunopotentiator of the present invention can be prepared in large quantities by a solid-phase chemical synthesis method, and mixed with the inactivated antigen at a certain ratio, which is convenient for large-scale production and preparation of a vaccine composition, which can induce the body to produce a humoral immune response level , can also enhance the body's cellular immune response level, and can obtain a better immune effect. The immunopotentiator of the invention is safe and reliable, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products. Specifically, the invention relates to an immune enhancer, a porcine foot-and-mouth disease vaccine composition containing the immune enhancer and its application. Background technique [0002] Foot and Mouth Disease (FMD) is an acute, febrile, highly contagious and rapid long-distance disease that occurs in pigs, cattle, sheep and other cloven-hoofed animals caused by Foot-and-Mouth Disease virus (FMDV). The spread of severe infectious diseases is known for its strong infectivity, rapid disease transmission, and serious harm. The outbreak of the disease has caused huge economic losses to the animal husbandry in the published area (Chang Huiyun, 2017). The World Organization for Animal Health (OIE) lists it as one of the multiple zoonotic infectious diseases that must be notified, and my country lists it as a first-class animal infectious disease. [0003] Foot-and-mouth d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61K39/135A61P31/14A61P37/04
CPCA61K38/08A61K39/12A61K2039/5252A61K2039/5258A61K2039/552A61K2039/572A61K2039/575A61P31/14A61P37/04C07K7/06A61K2300/00
Inventor 肖进王振豹巴利民张欣王楠董春娜张蕾赵洪涛张艳宾
Owner CHINA ANIMAL HUSBANDRY IND
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