Sexual attractant for grape moth and its preparation method and application

Abstract

The invention relates to the technical field of preparation of insect sex pheromone, in particular to a lobesia botrana sex attractant as well as a preparation method and an application thereof. The lobesia botrana sex attractant comprises (E,Z)-7,9-dodecenyl acetate (E7,Z9-12:Ac) and (Z)-9-dodecenyl acetate (Z9-12:Ac). The provided lobesia botrana sex attractant is simple to prepare, has diversified dose forms, is simple to use, has high trapping capability, good stability and high specificity, does not produce resistance, has no harm to enemies, is non-toxic to human and animals, environmentally friendly and long in lasting period and has the control effect maintained for 30 d or longer. The lobesia botrana sex attractant can be applied to pest quarantine, insect pest situation monitoring, mass trapping and mating disruption of lobesia botrana and has great significance in comprehensive control of the lobesia botrana.

Description

technical field

[0001] The invention relates to the technical field of preparation of insect sex pheromones, in particular to a sex attractant for the grape moth, a preparation method and application thereof. Background technique

[0002] Lobesia botrana (Denis et Schiffermuller), belongs to Lepidoptera (Lepidoptera), Tortricidae (Tortricidae), flower wing small tortrina (Lobesia), is a serious damage to a variety of economic plants flowers and It is a worldwide pest of fruit and is listed as an important entry plant quarantine pest in many countries in the world, including my country. The grape moth mainly feeds on the flowers and berries of grapes with its larvae. If mature grape bunches are attacked by moths, the berries will be infected with botrytis and further rot. In addition to grapes, the moth also affects olives, blackberries, cherries, nectarines, persimmons and pomegranates. Originating in Italy, the grape moth has spread throughout Europe, northern and western ...

Examples

Embodiment 1

[0017] Example 1 Preparation of Sexual Attractant (Rubber Lure Core) for Grape Toricula Moth

[0018] Table 1 shows the formulations of different sexual attractants (rubber lure cores) for grape moths. (E,Z)-7,9-dodecadien-1-ol acetate (E7,Z9-12:Ac) and (Z)-9-dodecene acetate (Z9-12 :Ac) were mixed in different proportions (the total amount was 1000 μg), dissolved in 500 μL of n-hexane, dropped into a 500 μL anti-mouth rubber tip, and air-dried to obtain the lure core of the sexual attractant of the grape moth.

[0019] Table 1 Formulas of different sexual attractants (rubber lures with anti mouth)

[0020]

Embodiment 2

[0021] Example 2 Preparation of Sexual Attractant (Polyethylene Bottle Lure)

[0022] The formulations of different grape moth sex attractants (polyethylene bottle lures) are shown in Table 2. (E,Z)-7,9-dodecadien-1-ol acetate (E7,Z9-12:Ac) and (Z)-9-dodecene acetate (Z9-12 :Ac) were mixed in different proportions (the total amount was 1000 μg), dissolved with 500 μL of chlorinated paraffin, added to a 1000 μL polyethylene bottle, and sealed to obtain the lure core of the sexual attractant for the grape moth.

[0023] Table 2 Formulas of different sexual attractants (polyethylene bottle lures) for the grape moth

[0024]

Embodiment 3

[0025] Example 3 Field test of sexual attractant (anti-mouth rubber lure core) for grape moth

[0026] Select grape variety, growing way, fertilizer and water management condition to be consistent, and the contiguous vineyards with an area of ​​more than 10 mu are used as test fields, and 8 are evenly set to contain the grape moth sex attractant (anti-mouth rubber lure core) in embodiment 1. Sticky insect plate trap (traps purchased from Zhangzhou Yinger Agricultural Science and Technology Co., Ltd.), set up sex attractant control (SP) and blank control (CK) at the same time, wherein neutral attractant contrast (SP) is to contain the following mass ratio Compounds of {(E,Z)-7,9-dodecadien-1-ol acetate (E7,Z9-12:Ac), (E,Z)-7,9-dodecadiene -1-alcohol (E7, Z9-12:OH) and (Z)-9-dodecene acetate (Z9-12:Ac) ratio of 100:5:1)} anti-mouth rubber with a content of 1000μg Sticky board traps with lure cores; blank control (CK) is sticky board traps without lure cores.

[0027] Three rep...

technical field

[0001] The invention relates to the technical field of preparation of insect sex pheromones, in particular to a sex attractant for the grape moth, a preparation method and application thereof. Background technique

[0002] Lobesia botrana (Denis et Schiffermuller), belongs to Lepidoptera (Lepidoptera), Tortricidae (Tortricidae), flower wing small tortrina (Lobesia), is a serious damage to a variety of economic plants flowers and It is a worldwide pest of fruit and is listed as an important entry plant quarantine pest in many countries in the world, including my country. The grape moth mainly feeds on the flowers and berries of grapes with its larvae. If mature grape bunches are attacked by moths, the berries will be infected with botrytis and further rot. In addition to grapes, the moth also affects olives, blackberries, cherries, nectarines, persimmons and pomegranates. Originating in Italy, the grape moth has spread throughout Europe, northern and western ...

Examples

Embodiment 1

[0017] Example 1 Preparation of Sexual Attractant (Rubber Lure Core) for Grape Toricula Moth

[0018] Table 1 shows the formulations of different sexual attractants (rubber lure cores) for grape moths. (E,Z)-7,9-dodecadien-1-ol acetate (E7,Z9-12:Ac) and (Z)-9-dodecene acetate (Z9-12 :Ac) were mixed in different proportions (the total amount was 1000 μg), dissolved in 500 μL of n-hexane, dropped into a 500 μL anti-mouth rubber tip, and air-dried to obtain the lure core of the sexual attractant of the grape moth.

[0019] Table 1 Formulas of different sexual attractants (rubber lures with anti mouth)

[0020]

Embodiment 2

[0021] Example 2 Preparation of Sexual Attractant (Polyethylene Bottle Lure)

[0022] The formulations of different grape moth sex attractants (polyethylene bottle lures) are shown in Table 2. (E,Z)-7,9-dodecadien-1-ol acetate (E7,Z9-12:Ac) and (Z)-9-dodecene acetate (Z9-12 :Ac) were mixed in different proportions (the total amount was 1000 μg), dissolved with 500 μL of chlorinated paraffin, added to a 1000 μL polyethylene bottle, and sealed to obtain the lure core of the sexual attractant for the grape moth.

[0023] Table 2 Formulas of different sexual attractants (polyethylene bottle lures) for the grape moth

[0024]

Embodiment 3

[0025] Example 3 Field test of sexual attractant (anti-mouth rubber lure core) for grape moth

[0026] Select grape variety, growing way, fertilizer and water management condition to be consistent, and the contiguous vineyards with an area of ​​more than 10 mu are used as test fields, and 8 are evenly set to contain the grape moth sex attractant (anti-mouth rubber lure core) in embodiment 1. Sticky insect plate trap (traps purchased from Zhangzhou Yinger Agricultural Science and Technology Co., Ltd.), set up sex attractant control (SP) and blank control (CK) at the same time, wherein neutral attractant contrast (SP) is to contain the following mass ratio Compounds of {(E,Z)-7,9-dodecadien-1-ol acetate (E7,Z9-12:Ac), (E,Z)-7,9-dodecadiene -1-alcohol (E7, Z9-12:OH) and (Z)-9-dodecene acetate (Z9-12:Ac) ratio of 100:5:1)} anti-mouth rubber with a content of 1000μg Sticky board traps with lure cores; blank control (CK) is sticky board traps without lure cores.

[0027] Three rep...

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