Application of asiaticoside in the treatment of abnormal bone metabolism

A technology of asiaticoside and osteoclasts, which is applied in the field of biomedicine to achieve the effect of low dosage

Active Publication Date: 2021-01-26
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no application of asiaticoside in the treatment of bone metabolism disorders

Method used

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  • Application of asiaticoside in the treatment of abnormal bone metabolism
  • Application of asiaticoside in the treatment of abnormal bone metabolism
  • Application of asiaticoside in the treatment of abnormal bone metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] 1 Asiaticoside inhibits RANKL-induced osteoclastogenesis

[0019] The structure and properties of asiaticoside such as figure 1 As shown in A.

[0020] For osteoclast culture, freshly isolated bone marrow macrophages (BMM) were isolated from the bones of C57BL / 6 mice, and the cells were flushed from the marrow cavity of the femur and tibia in a-MEM containing M-CSF. Convert the original BMM to 10 3 cells / well were inoculated into a 96-well plate with a-MEM and M-CSF (25ng / mL), and kept at 37°C in 5% CO 2 overnight. BMMs were stimulated with BSAKL (50 ng / mL) and different concentrations of asiaticoside (0.5, 1, 2.5, 5, 10, 20 μmol) every 2 days until osteoclasts formed. Fix with 4% paraformaldehyde in phosphate buffered saline (PBS) for 10 min at room temperature and wash 4 times with PBS. TRAcP activity was detected using the number of TRAcP-positive multinucleated cells. TRAcP-positive multinucleated cells with three or more nuclei were scored as osteoclast-like ...

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Abstract

The invention discloses asiaticoside, and a function of a pharmaceutically acceptable derivative thereof in bone metabolic abnormality. The asiaticoside has an adjustment function on simultaneously inhibiting osteoclast formation under the situation of bone metabolic abnormality, is low in effective dosage, and has a better effect on adjusting bone metabolic abnormality. The invention defines an intervention way of an asiaticoside related drug. The invention clarifies that a molecular mechanism in a bone microenvironment and a potential pharmacology intervention way are crucial to identifyinga novel treatment compound.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of asiaticoside in the treatment of abnormal bone metabolism. Background technique [0002] Abnormal bone metabolism (Osteoporosis) is a dynamic pathological condition caused by an imbalance between osteoblast bone formation and osteoclast bone resorption. Maintaining the link between osteoclasts and osteoblasts is critical for maintaining bone mass. Once osteoclast-induced bone resorption exceeds osteoblast-induced bone formation, it will progress to osteoporosis. Osteoporosis is primarily characterized by decreased bone mineral and structural disturbances in the skeletal system. Osteoclast and osteoblast differentiation and formation are regulated by several signaling pathways. For the osteoclast system, the receptor activator of NF-κB ligand (RANKL) is a member of the tumor necrosis factor family (TNF) mainly expressed by chondrocytes and osteocytes. RANKL is imp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7024A61P19/08
CPCA61K31/7024
Inventor 洪郭驹魏秋实韩晓蕊徐家科何伟陈镇秋
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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