Application of extract of root of Hainan devilpepper in treating of ulcerative colitis in mice
A technology of extract and Rauvolfia, applied in the field of the role and mechanism of dextran sodium sulfate in mice colitis, can solve problems such as unclear
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[0014] Example 1 The mechanism of anti-inflammatory immune effect of Hainan Rauwolfia extract pectin polysaccharide in treating UC
[0015] 1. Materials
[0016]1. Animals: 80 female BALB / C mice aged 6-8 weeks, weighing 17-22g per mouse, purchased from the Experimental Animal Center of West China Medical Center, Sichuan University, raised in the Human Disease-Related Peptide Laboratory, and raised in rack cages , keep constant temperature (23 ± 1 ℃), constant humidity (humidity 50-60%), light and darkness alternately for 12 hours, eat sterilized standard feed, and use sterilized filtered water.
[0017] 2. Drugs and reagents: Hainan Rauvolwolfia, collected from Mount Diaoluo, Hainan. Sodium dextran sulfate (dextran sulfatesodium, DSS molecular weight 36000-50000), produced by Sigma. IL-4, IL-13 kits, produced by Gibco. Rabbit anti-human IκB-α polyclonal antibody (Cayman Chemical Company, USA), and the above reagents were provided by Chengdu Genna Biotechnology Co., Ltd.
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