The invention discloses test paper for measuring
high density lipoprotein cholesterol and a preparation method and application thereof. The test paper comprises a
red blood cell separation layer, an auxiliary separation layer and a
reaction layer, wherein the
red blood cell separation layer, the auxiliary separation layer and the
reaction layer are prepared from a separation material, a separationmembrane and a reaction membrane through carrying out
steeping by
red blood cell separation solution, auxiliary separation solution and reaction solution and then carrying out
drying; the red
blood cell separation solution comprises the following components: buffer salt, a
divalent metal ion,
dextran sulfate sodium salt with a molecular weight of 30000-500000,
hemagglutinin or an Anti-RBC
antibody; the auxiliary separation solution comprises the following components: buffer salt, a surfactant I and polyhydric
alcohol; and the reaction solution comprises the following components: buffer salt,a surfactant II,
polysaccharide,
cholesterol esterase,
cholesterol oxidase,
peroxidase,
ascorbic acid oxidase, an oxidizing substance and a color developing agent. The test paper is capable of simplyand rapidly detecting the contents of
high density lipoprotein cholesterol. The invention furthermore provides a preparation method and application of the test paper.