Hydroponic culture method for rice underground stems

A rhizome and hydroponics technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve problems such as restricting upward growth, unfavorable rhizome test observation, and inability to accept sunlight, etc., to achieve sufficient phenotype, convenient and flexible operation , to facilitate continuous observation of the effect

Active Publication Date: 2018-07-06
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except for a small number of openings, the traditional hydroponic fixed boards are all closed and solid, which limits the upward growth of underground stems after they come out of the water. The aboveground stems develo

Method used

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  • Hydroponic culture method for rice underground stems
  • Hydroponic culture method for rice underground stems
  • Hydroponic culture method for rice underground stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A hydroponic method for rice underground stems, comprising the steps of:

[0038] (1) according to the operation in step 1, get the long stamen wild rice ground stem with good growth condition from the field, cut off the blade and root, and strip off the leaf sheath;

[0039] (2) Take a 2L beaker and add 500ml of distilled water first, then add 6-BA or KT stock solution to prepare the treatment solution. The treatment solution contains 6-BA or KT at a final concentration of 0.5-2mg / L. Soak the rice stems of step (1) in a beaker containing 500ml of culture solution, and seal the mouth of the beaker with a plastic film with air holes, place the shaker at 100-200rpm / min at room temperature for 1- 3d; During this period, the treatment solution was changed once a day;

[0040] (3) After the rice stem germinates, take it out from the culture medium, rinse it with clear water, and soak it in clear water for 10-12 hours to remove 6-BA or KT (6-BA or KT in step (2) will inhibi...

Embodiment 2

[0046] A hydroponic method for rice underground stems, comprising the steps of:

[0047] (1) After drying the harvested O. sativa seeds, place them in a blast drying oven at 50 degrees Celsius and dry them for five days to break dormancy;

[0048] (2) Peel off the chaff carefully of the seeds that have broken dormancy (be careful not to hurt the seeds);

[0049] (3) Soak the husk-removed seeds in an aqueous solution containing 1 / 1000 concentration of carbendazim for 2-5 hours, transfer to clean water and soak for 1 day;

[0050] (4) Rinse the soaked seeds with clean water, wrap them with moist filter paper, and place them in an incubator at 37 degrees Celsius for 1-2 days to accelerate germination. During this period, keep the filter paper moist to prevent the seeds from dehydrating;

[0051] (5) Sow the germinated seeds into the paddy soil, and place them at room temperature to grow, and pay attention to timely watering during the period to prevent the seeds from dehydrating...

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Abstract

The invention belongs to the technical field of rice cultivation and particularly relates to a hydroponic culture method for rice underground stems. The method mainly includes seedling acquisition andgrid hydroponic culture. Seedlings are obtained by germination of seeds or induced germination of stem-node dormant buds. The induced germination of the stem-node dormant buds is achieved by means ofthe method, wherein the stems (overground stems or underground stems) with nodes are placed in a treatment liquid containing 0.5-2 mg/L 6-BA (6-benzyl adenine) or KT (kinetin) and then placed in a shaker for shaking culture at 100-200 rpm/min for 1-3 days, the treatment liquid is replaced once a day, and the treatment is stopped after germination of the stem-node buds; after the seedlings grow toa 3-5 leaf stage, hydroponic culture is started, and a metal mesh and a sponge block are adopted as a hydroponic fixing device; the seedlings are clamped by using the sponge block and then loaded into grids of the metal mesh to be perpendicular to the liquid surface; a 0.5* nutrient solution of the international rice research institute is adopted for recovering culture for 5-10 days and then replaced with a 1* nutrient solution for continuous culture. Through a grid fixing method, the advantages of convenient observation and easy control over experimental conditions of the hydroponic cultureare fully utilized, and a research is conducted on the development of the rice underground stems.

Description

technical field [0001] The invention belongs to the technical field of rice cultivation, in particular to a hydroponic method for rice underground stems. Background technique [0002] The rhizome of rice is a very important and complex trait, and the research on its growth and development is not yet in-depth. Under natural conditions, the underground stems of rice are buried in the soil, and it is much more difficult to study their growth and development than the aboveground stems. Nowadays, the observation of rice underground stems is generally done by digging out the whole rice plant or only taking the underground stem parts for observation and research. Excavation will Damage to rice changes the original growth state of rice, and the development process of rice rhizome cannot be continuously observed. [0003] The hydroponic (liquid culture) method is not only convenient to observe the development dynamics of rhizomes, but also can precisely control the culture condition...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/00
CPCA01G7/00A01G31/00
Inventor 杨江义王凯范优荣范智权
Owner GUANGXI UNIV
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