Hydroponic culture method for rice underground stems
A rhizome and hydroponics technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve problems such as restricting upward growth, unfavorable rhizome test observation, and inability to accept sunlight, etc., to achieve sufficient phenotype, convenient and flexible operation , to facilitate continuous observation of the effect
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Embodiment 1
[0037] A hydroponic method for rice underground stems, comprising the steps of:
[0038] (1) according to the operation in step 1, get the long stamen wild rice ground stem with good growth condition from the field, cut off the blade and root, and strip off the leaf sheath;
[0039] (2) Take a 2L beaker and add 500ml of distilled water first, then add 6-BA or KT stock solution to prepare the treatment solution. The treatment solution contains 6-BA or KT at a final concentration of 0.5-2mg / L. Soak the rice stems of step (1) in a beaker containing 500ml of culture solution, and seal the mouth of the beaker with a plastic film with air holes, place the shaker at 100-200rpm / min at room temperature for 1- 3d; During this period, the treatment solution was changed once a day;
[0040] (3) After the rice stem germinates, take it out from the culture medium, rinse it with clear water, and soak it in clear water for 10-12 hours to remove 6-BA or KT (6-BA or KT in step (2) will inhibi...
Embodiment 2
[0046] A hydroponic method for rice underground stems, comprising the steps of:
[0047] (1) After drying the harvested O. sativa seeds, place them in a blast drying oven at 50 degrees Celsius and dry them for five days to break dormancy;
[0048] (2) Peel off the chaff carefully of the seeds that have broken dormancy (be careful not to hurt the seeds);
[0049] (3) Soak the husk-removed seeds in an aqueous solution containing 1 / 1000 concentration of carbendazim for 2-5 hours, transfer to clean water and soak for 1 day;
[0050] (4) Rinse the soaked seeds with clean water, wrap them with moist filter paper, and place them in an incubator at 37 degrees Celsius for 1-2 days to accelerate germination. During this period, keep the filter paper moist to prevent the seeds from dehydrating;
[0051] (5) Sow the germinated seeds into the paddy soil, and place them at room temperature to grow, and pay attention to timely watering during the period to prevent the seeds from dehydrating...
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