Chromosome configuration capture library and its construction method

Abstract

The invention discloses a chromosome configuration capture library and a construction method thereof. The construction method comprises the following steps: the construction method comprises preparing a tissue sample into a single-cell suspension; performing cross-linking and fixing on the single-cell suspension to obtain a DNA-protein cross-linking complex; obtaining a DNA-protein cross-linking complex DNA fragments were used for library construction to obtain a chromosome configuration capture library. By first preparing the tissue sample into a single-cell suspension and then performing cross-linking fixation, the problem that the effect of tissue cross-linking and fixation is seriously affected by the size of the tissue is avoided, and the proportion of small fragments in the library is also avoided due to excessive cross-linking and fixation of some tissues. , a phenomenon in which information about the interaction between DNA and proteins in chromosome configuration decreases. It avoids the problem of inaccurate capture of chromosome conformation caused by changes in cell morphology, improves the stability and universality of libraries constructed from multiple tissues, and greatly saves manpower and reagent costs.

Description

technical field

[0001] The invention relates to the field of high-throughput sequencing library construction, in particular to a chromosome configuration capture library and a construction method thereof. Background technique

[0002] With the development of sequencing technology, human beings have more and more thorough understanding of biological species information, but resequencing is usually based on the reference genome for variation information detection, and chromosomes are carriers of genetic material, and their activity and function are determined by structure and spatial conformation Co-determined, the three-dimensional conformation of chromosomes has also become a representative technology for genome research. Hi-C technology is derived from the Chromosome conformation capture (3C) technology, which takes the entire nucleus as the research object, uses high-throughput sequencing technology, and combines biological The information analysis method studies the relat...

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