Micro-fluidic chip system capable of regulating emulsion formation

Abstract

The invention provides a micro-fluidic chip system capable of regulating emulsion formation. The micro-fluidic chip system comprises a micro-fluidic chip and a control mechanism arranged outside the chip, wherein a plurality of material loading holes and a plurality of liquid drop collecting holes are formed in the micro-fluidic chip; the material loading holes consist of oil holes and sample holes, the oil holes and the sample holes communicate with the liquid drop collecting holes through corresponding pipelines respectively, and pressure regulating devices are arranged at the inlet end of the oil hole and the inlet end of the sample hole respectively. The system has the beneficial effects that liquid entering the oil holes and the sample holes can be independently controlled, so that the sizes of formed liquid drops are regulated and controlled; independent group control is carried out on the pipeline in pairs, selective opening and closing of passages are carried out, and the wasteof energy sources and consumed materials cannot be caused; and the internal blockage of chip pipelines is avoided by adding a filtering mechanism. The proportion of oil to water can be controlled tobe (1 to 6):1 by adopting the system, and the oil consumption is greatly saved under the condition that the same number of liquid drops needs to be produced.

Description

technical field [0001] The invention belongs to a high-throughput analysis system, in particular to a microfluidic chip system capable of regulating emulsion formation. Background technique [0002] The oil-water two-phase microemulsion has been used in high-throughput analysis systems, and its application range has a tendency to expand. For example, the emulsion is formed for the combination of polymerase chain reaction (PCR), and a single copy of the nucleic acid target molecule is amplified in the droplet, so as to realize the detection of the existence of the single molecule of the target and the specific gene sequence. 10X Genomics, an American company, uses the emulsion PCR method combined with GemCode technology to generate long-chain DNA, thereby realizing long-chain DNA sequence sequencing or single-cell sequencing. It has been recognized by the industry as one of the best technologies for long-chain DNA sequencing and single-cell sequencing. one. And the emulsion...

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Problems solved by technology

However, the above system is limited by the resistance of the microfluidic pipeline, the ratio of the oil phase reagent to the sample is basically fixed, and the size of the generated droplets is also basically fixed.

At the same time, Biorad's emulsion generation microfluidic chip has 8 channels (emulsion preparation of 8 samples can be carried out at the same time), which also leads to the use of the chip system must do 8 channels at the same time, when the user has less than 8 samples In some cases, the remaining channels must be filled with oil or buffer, resulting in a waste of resources in the use of channels

In addition, if Biorad's microfluidic chip uses unfiltered samples, it is easy to cause blockage of the pipeline, which increases the operation steps and indirectly increases the processing cost

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