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Preparation method and application of Leersia hexandra swartz endophytic bacteria with hexavalent chromium reducing function

A technology of endophytic bacteria and hexavalent chromium, applied in the field of preparation of Lishihe endophytic bacteria

Active Publication Date: 2018-07-20
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the reduction of heavy metal Cr(VI) by endophytic bacteria of Lystia chinensis.

Method used

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  • Preparation method and application of Leersia hexandra swartz endophytic bacteria with hexavalent chromium reducing function
  • Preparation method and application of Leersia hexandra swartz endophytic bacteria with hexavalent chromium reducing function
  • Preparation method and application of Leersia hexandra swartz endophytic bacteria with hexavalent chromium reducing function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation and screening of Li's Wo chromium-reducing endophytic bacteria

[0023] First, collect Li's gracilis plants from the Phytoremediation Laboratory of the School of Environmental Science and Engineering, Guilin University of Technology, take an appropriate amount of healthy Li's graham roots, rinse them with water, soak them with 70% alcohol for 40s under sterile conditions, and then use 2.5% sodium hypochlorite. Soak for 2 min, and finally rinse with sterile water 6 times to remove the disinfectant adhering to the surface of the material. Under aseptic conditions, the beef extract peptone solid plate medium was applied to the beef extract peptone solid plate medium with the sterile water rinsed for the last time, and no microorganisms grew after culturing, indicating that the surface was thoroughly disinfected. Take an appropriate amount of surface-sterilized root tissue under aseptic conditions, add 1 mL of 0.9% sodium chloride solution to fully grind...

Embodiment 2

[0027] The effect of embodiment 2Cr(VI) concentration on the growth of G04 strain

[0028] After activating the strain stored on the slant of the test tube, pick a ring and inoculate it into a 100 mL conical flask containing 40 mL of liquid medium, and use it as a seed solution after shaking at 37 °C and 120 r / min for 24 h. The seed liquid was inoculated into beef extract peptone liquid medium (100mL / 250mL conical flask) with Cr(VI) concentrations of 50mg / L, 100mg / L and 200mg / L respectively according to 10% inoculation amount, and placed in a constant temperature of 37 °C. Shake culture at 120 r / min on a horizontal shaker. During the incubation period, 5 mL was sampled under sterile conditions at certain intervals, and the optical density OD value at a wavelength of 600 nm was measured. The results are attached figure 1 shown. When the concentration of Cr(VI) was added to the medium at 50 mg / L, 100 mg / L and 200 mg / L, the growth trend of the strain was similar to that of the...

Embodiment 3

[0029] Example 3 Effect of initial pH on reduction of Cr(VI) by G04 strain

[0030] The strain G04 stored on the slant of the test tube was activated by the beef extract peptone solid medium plate, and after culturing in a constant temperature incubator at 37 °C for 24 hours, two loops were picked and inoculated into a 250 mL conical flask containing 100 mL of beef extract peptone liquid medium. 120r / min shaking culture for 24h as seed solution.

[0031] The seed liquid was inoculated into the beef extract peptone liquid medium containing Cr(VI) concentration of 100mg / L (80mL / 250mL conical flask) according to 15% inoculum, and the pH was adjusted with 2M sodium hydroxide and hydrochloric acid solution. After that, the eight layers of gauze were sealed and placed on a constant temperature horizontal shaker at 37°C for 120 r / min shaking culture. After culturing for 48 h, an appropriate amount of culture medium was sampled under aseptic conditions, centrifuged at 10,000 r / min fo...

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Abstract

The invention discloses a preparation method and application of Leersia hexandra swartz endophytic bacteria with a hexavalent chromium reducing function. The strain has the classification naming of Enterobacter cloacae, and is collected in CGMCC (China General Microbiological Culture Collection Center) with the address of NO.3, NO.1 Yard, Beichen West Road, Chaoyang District, Beijing, 100101. Thecollection number is CGMCC NO.14265. The Leersia hexandra swartz endophytic bacteria are separated and screened from the root tissues of the chromium super accumulation plants of Leersia hexandra swartz, are identified as Enterobacter cloacae, and are named as G04. The strain can reduce hexavalent chromium into trivalent chromium; the chromium reducing conditions are cleared; the strain resource and the theoretical basis are provided for hexavalent chromium pollution microbial remediation.

Description

technical field [0001] The present invention relates to a kind of bacterial strain that can be used for environmental pollution control, particularly relate to a kind of preparation method and application of the endophytic bacteria of Rhizoma Rhizoma with reducing hexavalent chromium. Background technique [0002] With the development of modern technology, heavy metal chromium has been widely used in many fields such as pharmaceuticals and electroplating. But at the same time, the pollution of chromium and its compounds in the environment is becoming more and more serious. Long-term exposure to chromium and chromium compounds can easily affect the gastrointestinal system, immune system, liver and kidneys and even induce respiratory cancer. Among the various chromium forms, Cr(VI), mainly in the chromate and dichromate forms, is considered to be the most toxic. Internationally, bioremediation is advocated for chromium-contaminated water and soil, that is, the removal of hea...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34B09C1/10C12R1/01C02F101/22
CPCB09C1/10C02F3/34C02F2101/22C12N1/02C12N1/20
Inventor 李海云袁治豪郑里华陈慧英李培骏李霞
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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