Cell enriching system and method

Abstract

The invention discloses a cell enriching system and a cell enriching method. The cell enriching system comprises a sample bottle, a loading mechanism, a uniformly mixing mechanism, a concentrating anduniformly mixing mechanism and a controller, wherein the loading mechanism can convey the sample bottle; the uniformly mixing mechanism can perform a first uniformly mixing operation on the sample bottle; the concentrating and uniformly mixing mechanism can pierce the bottom of the bottle, and perform sucking and reverse blowing operations on the sample bottle; the controller controls the loadingmechanism to convey the sample bottle to a uniformly mixing position, and then the uniformly mixing mechanism performs the first uniformly mixing operation; after the controller controls the loadingmechanism to convey the sample bottle to a concentrating position, the controller controls the concentrating and uniformly mixing mechanism to pierce the bottom of the bottle to enter a second cavityand repeatedly perform the sucking and reverse blowing operations. The cell enriching system provided by the invention achieves cell concentration by performing the sucking and reverse blowing operations, so that a filter device of the sample bottle and a concentrating needle can be effectively prevented from being blocked, cell superposition can be effectively reduced while cell concentration isaccelerated, and thus the cell enriching quality is improved.

Description

technical field [0001] The invention relates to the technical field of medical bioassays, and more specifically relates to a cell enrichment system and method. Background technique [0002] In medical testing, it is often necessary to enrich a specific cell in the specimen, and then identify the cells. In order to ensure the accuracy of the test and the number of cells required for the test, it is usually necessary Enrichment is carried out under certain conditions, which can prevent infection and pollution, and ensure the safety of operators and the surrounding environment. [0003] At present, liquid-based products on the market adopt two methods for cell enrichment: one is to enrich cells by centrifugation after shaking and mixing. The operation process is as follows: manually shake the sample in the vortex shaker, and then manually place the mixed sample on the sample transfer machine to transfer the sample to the centrifuge tube, and then put the centrifuge tube into t...

AI Technical Summary

Problems solved by technology

Because the cells are directly adsorbed on the membrane by negative pressure, the cells will be unevenly distributed on the surface of the membrane. When the cells on the membrane are directly pressed on the glass slide, there is a risk of cell overlap in the preparation of the slide.

Although this method saves a lot of manual operations, the overlap and loss of cells is uncontrollable

Images