Novel visualization quantitative detection method for dual heavy metal ions

Abstract

The invention provides a novel dual colorimetric sensing method based on a dual super-fast PCR. The method is used for Hg2+ and Ag+ visualization ultra-sensitive dual detection. The novel method comprises the steps that according to mispairing of mercury ion thymine, and mispairing of silver ion cytosine, a dual amplification primer and template of a super-fast polymerase chain reaction (PCR) aredesigned, meanwhile, the primer can be combined with an enzyme united nucleic acid self-assembly developing module, the novel dual target function nucleic acid detecting method and a biosensor based on mispaired mercury ions and silver ions are integrated and built. The built detection method and biosensor are faster and more sensitive, have the advantages of being high in specificity and sensitivity, reliable in detection result and the like compared with a traditional method, the analysis detection steps can be simplified, the analysis time can be shortened, more importantly, online real-time detection becomes possible, carrying and field operation are facilitated, and the method has the good application prospect in the field of rapid detection of heavy metals.

Description

Technical field [0001] The invention belongs to the technical field of biological detection, and specifically relates to a double metal detection method and a biosensor. Background technique [0002] Mercury in nature mainly exists in three forms, including: elemental mercury (Hg), organic mercury (alkyl mercury, phenyl mercury) and inorganic mercury (Hg + And Hg 2+ Salt and its complexes), and various substances can undergo biological and chemical conversion. It is the only metal that exists in liquid form at room temperature. Its physical shape is silver-white, melting point -38.87°C, boiling point 356.6°C, density 13.59 g / cm3, and the element symbol is Hg. Silver has a simple substance state, but generally exists in silver ore in a chemical state. It presents a white shiny form, with a melting point of 961.78°C, a boiling point of 2212°C, a density of 10.49 g / cm3, and the element symbol is Ag. [0003] Traditional Hg 2+ And Ag + The detection methods mainly include atomic abso...

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Problems solved by technology

[0003] Traditional Hg 2+ and Ag + The detection methods mainly include atomic absorption spectrometry (AAS), atomic fluorescence spectrometry (AFS), inductively coupled plasma mass spectrometry, electrochemical analysis methods, atomic absorption spectrophotometry, etc. These detection methods have high sensitivity, wide detection range, It is suitable for the analysis of various samples; however, due to the use of large instruments, high maintenance costs and professional operation are required to increase the detection cost; at the same time, the sample pretreatment process is complicated and some require the use of corrosive reagents; the detection cycle is long; due to the above shortcomings , so that the traditional method is not suitable for on-site analysis lacking precision instruments and other shortcomings, it is difficult to meet the requirements of actual analysis, etc.

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