Detection reagent for procalcitonin colloidal gold by immunoturbidimetry
An immunoturbidimetric and detection reagent technology, applied in the field of medicine, can solve problems such as pollution of the environment, and achieve the effects of no environmental pollution, long shelf life, and significant economic and social benefits.
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Embodiment 1
[0011] A procalcitonin colloidal gold immunoturbidimetric detection reagent of the present invention comprises reagent 1 and reagent 2, and described reagent 1 is made of trishydroxymethylaminomethane (Tris) 6.05g, sodium azide (NaN 3 ) 0.5g, add water to 1000ml and mix evenly, and adjust the pH value to 8.15 with hydrochloric acid to make; the reagent 2 is made of tris (Tris) 6.05g, bovine serum albumin (BSA) 1g, polyethylene Pyrrolidone K30 (PVP-K30) 0.5g, polyethylene glycol 20000 (PEG20000) 0.5g, Tween-20 (Tween-20) 2ml, sucrose 35g, sodium azide (NaN 3 ) 0.5g, casein 0.5g, add water to 1000ml and mix well, adjust the pH value to 8.15 with hydrochloric acid to obtain a buffer solution, then dissolve the colloidal gold particles labeled with anti-human procalcitonin antibody with the buffer solution, the ratio is 11 mg antibody / 1L colloidal gold solution, adjust the concentration to 520nm absorbance (OD) is 0.2, obtains reagent 2.
Embodiment 2
[0013] A procalcitonin colloidal gold immunoturbidimetric detection reagent of the present invention comprises reagent 1 and reagent 2, and described reagent 1 is made of trishydroxymethylaminomethane (Tris) 6.05g, sodium azide (NaN 3 ) 1.0g, add water to 1000ml and mix evenly, and adjust the pH value to 8.25 with hydrochloric acid to make; the reagent 2 is made of tris (Tris) 6.05g, bovine serum albumin (BSA) 5g, polyethylene Pyrrolidone K30 (PVP-K30) 1.0g, polyethylene glycol 20000 (PEG20000) 1.0g, Tween-20 (Tween-20) 5ml, sucrose 50g, sodium azide (NaN 3 ) 1.0g, casein 1.25g, add water to 1000ml and mix well, adjust the pH value to 8.25 with hydrochloric acid to obtain a buffer solution, then dissolve the colloidal gold particles labeled with anti-human procalcitonin antibody with the buffer solution, the ratio is 11 mg antibody / 1L colloidal gold solution, adjust the concentration to 520nm absorbance (OD) is 0.25, obtains reagent 2.
Embodiment 3
[0015] A procalcitonin colloidal gold immunoturbidimetric detection reagent of the present invention comprises reagent 1 and reagent 2, and described reagent 1 is made of trishydroxymethylaminomethane (Tris) 6.05g, sodium azide (NaN 3 ) 1.5g, add water to 1000ml and mix evenly, and adjust the pH value to 8.35 with hydrochloric acid to make; the reagent 2 is made of tris (Tris) 6.05g, bovine serum albumin (BSA) 10g, polyethylene Pyrrolidone K30 (PVP-K30) 1.5g, polyethylene glycol 20000 (PEG20000) 1.5g, Tween-20 (Tween-20) 10ml, sucrose 65g, sodium azide (NaN 3 ) 1.5g, casein 2g, add water to 1000ml and mix well, adjust the pH value to 8.35 with hydrochloric acid to obtain a buffer solution, then dissolve the colloidal gold particles labeled with anti-human procalcitonin antibody with the buffer solution, the ratio is 11 mg antibody / 1L colloidal gold solution, adjust the concentration to 520nm absorbance (OD) is 0.3, obtain reagent 2.
[0016] This operation method can be used...
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