In vitro identification method of coffee germplasm rust resistance
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An identification method and resistance technology, which is applied in the field of in vitro identification of coffee germplasm rust resistance, can solve problems such as none, reduce experimental errors, facilitate popularization, and improve work efficiency
Inactive Publication Date: 2018-09-04
YUNNAN DEHONG TROPICAL AGRI RES INST
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Problems solved by technology
In order to improve the accuracy of rust resistance identification, in vitro leaf identification methods have been established on wheat and soybean, but there is no method and technology for in vitro rust identification on coffee
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Embodiment 1
[0031] A preparation method for rust fungus urediospores for in vitro identification of coffee rust resistance, comprising the steps of:
[0032] 1) Preparation of the inoculation culture box: Soak the sponge with 72% alcohol for 4 minutes, wherein the sponge is 1 cm thick, 15 cm long, and 10 cm wide, then air-dried, spread on the bottom of the plastic culture box, and soak the sponge with sterile water;
[0033] 2) From the field in the main coffee producing area of Yingjiang County, Yunnan Province, collect coffee leaves with typical coffee rust spots, gently scrape off the uredospores of the rust fungus with a scalpel, and apply the uredospores of the rust fungus on the affected areas. The diseased variety Matari has just unfolded, and the back of the young and soft leaves is lightly brushed with a brush to evenly distribute the uredia spores of the rust fungus, and sprayed with water mist until a water film is formed, then the back of the inoculated leaves faces upwards, ...
Embodiment 2
[0045] A kind of rust fungus urediospores that is used for in vitro identification of coffee rust resistance, the preparation method of described rust fungus uredospores is as follows:
[0046] 1) Preparation of the inoculation culture box: Soak the sponge with 75% alcohol for 5 minutes, wherein the sponge is 1 cm thick, 15 cm long, and 10 cm wide, then air-dried, spread on the bottom of the plastic culture box, and soak the sponge with sterile water;
[0047] 2) From the field in the main coffee producing area of Yingjiang County, Yunnan Province, collect coffee leaves with typical coffee rust spots, gently scrape off the uredospores with a scalpel, and apply the uredospores of the rust fungus to the susceptible varieties Caturra has just unfolded, and the back of the young and soft leaves is lightly brushed with a brush to evenly distribute the uredia spores of the rust fungus, and sprayed with water mist until a water film is formed, then the back of the inoculated leaves ...
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Abstract
The invention discloses an in vitro identification method of coffee germplasm rust resistance. The in vitro identification method of coffee germplasm rust resistance comprises steps of hemileia vastatrix uredospore preparation, in vitro leaf preparation, and inoculation identification; the step of hemileia vastatrix uredospore preparation comprises following steps: an inoculation culture box is prepared, hemileia vastatrix uredospores on coffee leaves with typical coffee rust bacterial plaques are collected, and are used for coating the back surfaces of leaves of susceptible varieties, and purified hemileia vastatrix bacterial strains are obtained through repeat inoculation. The in vitro identification method of coffee germplasm rust resistance is capable of ensuring the inoculation conditions, the inoculation pressure, and the incidence conditions of each germplasm materialfor inoculation to be highly consistent, reducing experimental errors caused by the inconformity of inoculation environment and incidence environment as far as possible, increasing identification result accuracy, and in addition, dependence of field identification on season conditions is avoided, rust resistanceidentification can be realized at any time, and working efficiency is increased greatly.
Description
technical field [0001] The invention relates to the technical field of disease resistance breeding, in particular to an in vitro identification method for coffee germplasm rust resistance. Background technique [0002] Coffee rust caused by coffee rust (Hemileia vastatrix Berk. & Br.) is a common disease in coffee (Coffea arabica) production, which seriously threatens the yield and quality of coffee in my country, causing a loss of 15% to 80% of coffee yield every year. %between. The disease occurs in coffee-producing areas all over the world, and it mainly harms coffee leaves, causing leaves to fall off, plants to weaken, and even die. Because coffee rust is an airborne disease, it is difficult to eradicate it by means of chemical control and biological control. Breeding disease-resistant varieties is the most economical and effective way to control coffee rust. [0003] However, at present, the identification of coffee rust mainly adopts the methods of natural identificat...
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